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ZO-1抗体 [N2C1], Internal

KO/KD Orthogonal
Anti-ZO-1 antibody [N2C1], Internal used in Western Blot (WB). GTX108627
Anti-ZO-1 antibody [N2C1], Internal used in Western Blot (WB). GTX108627
Anti-ZO-1 antibody [N2C1], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108627
Anti-ZO-1 antibody [N2C1], Internal used in Western Blot (WB). GTX108627
Anti-ZO-1 antibody [N2C1], Internal used in Western Blot (WB). GTX108627
Anti-ZO-1 antibody [N2C1], Internal used in Western Blot (WB). GTX108627

目录号 GTX108627

文献引用 7
1

宿主

 Rabbit

克隆

 Polyclonal

同种型

 IgG

实验应用

 WB, ICC/IF, IHC-Fr

种属反应

 Human, Rat
产品包装
100 μl (¥4000),
25 μl (¥1700)
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产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-Fr Assay dependent
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

195 kDa. ( Note )

阳性对照

HeLa , H1299 , HCT-116

预测种属反应

Mouse, Dog(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

0.98 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the center region of human ZO-1. The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_10731582

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

tight junction protein 1 , ZO-1

细胞定位

Cell membrane; Peripheral membrane protein; Cytoplasmic side , Cell junction , tight junction

功能与背景

This gene encodes a protein located on a cytoplasmic membrane surface of intercellular tight junctions. The encoded protein may be involved in signal transduction at cell-cell junctions. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq]

数据库

研究领域

產品图片

Anti-ZO-1 antibody [N2C1], Internal used in Western Blot (WB). GTX108627

GTX108627 WB Image

KO/KD

Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ZO-1 antibody [N2C1], Internal (GTX108627) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ZO-1 antibody [N2C1], Internal used in Western Blot (WB). GTX108627

GTX108627 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ZO-1 antibody [N2C1], Internal (GTX108627) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ZO-1 antibody [N2C1], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108627

GTX108627 ICC/IF Image

ZO-1 antibody [N2C1], Internal detects TJP1 protein at junction by confocal immunofluorescent analysis.
Sample: A431 cells were fixed in ice-cold MeOH for 5 min.
Green: TJP1 protein stained by ZO-1 antibody [N2C1], Internal (GTX108627) diluted at 1:500.
Blue: Hoechst 33342 staining.
[Images captured by Olympus FV10i Confocal Laser Scanning Microscope]

Anti-ZO-1 antibody [N2C1], Internal used in Western Blot (WB). GTX108627

GTX108627 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ZO-1 antibody [N2C1], Internal (GTX108627) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ZO-1 antibody [N2C1], Internal used in Western Blot (WB). GTX108627

GTX108627 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ZO-1 antibody [N2C1], Internal (GTX108627) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ZO-1 antibody [N2C1], Internal used in Western Blot (WB). GTX108627

GTX108627 WB Image

Orthogonal

The data was published in the 2019 in Cancers (Basel). PMID: 31861872

文献引用

Application Reference

Kobayashi K et al. Tissue Barriers 2024; 12 (1) : 2184157 Yogurt starter strains ameliorate intestinal barrier dysfunction via activating AMPK in Caco-2 cells.
Wang HK et al. J Inflamm Res 2023; 16 : 4103-4116 HDAC1 is Involved in Neuroinflammation and Blood-Brain Barrier Damage in Stroke Pathogenesis.
Wang HK et al. J Inflamm Res 2023; 2023 (16) : 4103—4116 HDAC1 is Involved in Neuroinflammation and Blood-Brain Barrier Damage in Stroke Pathogenesis
实验应用 : IHC-Fr
种属反应 : Rat
Takino JI et al. Sci Rep 2021; 11 (1) : 2959 RasGRP2 inhibits glyceraldehyde-derived toxic advanced glycation end-products from inducing permeability in vascular endothelial cells.
实验应用 : WB, ICC/IF
种属反应 : Human
Michielin F et al. Cell Rep 2020; 33 (9) : 108453 The Microfluidic Environment Reveals a Hidden Role of Self-Organizing Extracellular Matrix in Hepatic Commitment and Organoid Formation of hiPSCs.
实验应用 : ICC/IF
种属反应 : Human
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产品评论

ZO-1 antibody [N2C1], Internal 目录号 GTX108627

Rating
( Average 4 based on 1 users reviews)
实验应用
Western Blot(WB)
( Average 4 based on 1 users reviews)
Anti-ZO-1 antibody [N2C1], Internal used in Western Blot (WB). GTX108627
Date : Anonymous submitted on 24-Jun-2015
Score :
Application Tested : WB
Sample Species : Hu
Sample : HaCaT cells
Amount used : 20μg
Blocking : 5% non-fat milk, 25°C, 1Hr
Primary Antibody : 1:2000, 4°C, 16Hr
我要评论产品
SDS
PBS.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
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