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ZO-1抗体

KO/KD Orthogonal
Anti-ZO-1 antibody used in Western Blot (WB). GTX108592
Anti-ZO-1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108592
Anti-ZO-1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108592
Anti-ZO-1 antibody used in Western Blot (WB). GTX108592
Anti-ZO-1 antibody used in Western Blot (WB). GTX108592
Anti-ZO-1 antibody used in Western Blot (WB). GTX108592
Anti-ZO-1 antibody used in IHC (Paraffin sections) (IHC-P). GTX108592
Anti-ZO-1 antibody used in IHC (Paraffin sections) (IHC-P). GTX108592
Anti-ZO-1 antibody used in Western Blot (WB). GTX108592
Anti-ZO-1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX108592

目录号 GTX108592

文献引用 18
1

宿主

 Rabbit

克隆

 Polyclonal

同种型

 IgG

实验应用

 WB, ICC/IF, IHC-P, IHC-Fr

种属反应

 Human, Mouse, Bovine, Dog, Pig, Monkey
产品包装
100 μl (¥4000),
25 μl (¥1700)
查看所有 ZO-1 的产品

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
IHC-Fr Assay dependent

Note :

WB
The use of GTX108592 for mouse is based on IHC-P testing. For WB using mouse samples, we recommend GTX108613
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

195 kDa. ( Note )

阳性对照

293T , A431 , HeLa , HepG2

预测种属反应

Rat(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS, 1% BSA, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

0.19 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the center region of human ZO-1. The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_1952260

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

tight junction protein 1 , ZO-1

细胞定位

Cell membrane; Peripheral membrane protein; Cytoplasmic side , Cell junction , tight junction

功能与背景

This gene encodes a protein located on a cytoplasmic membrane surface of intercellular tight junctions. The encoded protein may be involved in signal transduction at cell-cell junctions. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq]

数据库

研究领域

產品图片

Anti-ZO-1 antibody used in Western Blot (WB). GTX108592

GTX108592 WB Image

KO/KD

Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ZO-1 antibody (GTX108592) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ZO-1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108592

GTX108592 ICC/IF Image

ZO-1 antibody detects ZO-1 protein at cell membrane and cytoplasm by immunofluorescent analysis.
Sample: MDCK cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ZO-1 stained by ZO-1 antibody (GTX108592) diluted at 1:1000.

Anti-ZO-1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108592

GTX108592 ICC/IF Image

ZO-1 antibody detects ZO-1 protein at cell membrane and cytoplasm by immunofluorescent analysis.
Sample: MDCK cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ZO-1 stained by ZO-1 antibody (GTX108592) diluted at 1:1000.

Anti-ZO-1 antibody used in Western Blot (WB). GTX108592

GTX108592 WB Image

Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with ZO-1 antibody (GTX108592) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ZO-1 antibody used in Western Blot (WB). GTX108592

GTX108592 WB Image

Mouse tissue extract (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with ZO-1 antibody (GTX108592) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ZO-1 antibody used in Western Blot (WB). GTX108592

GTX108592 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ZO-1 antibody (GTX108592) diluted at 1:1000.

Anti-ZO-1 antibody used in IHC (Paraffin sections) (IHC-P). GTX108592

GTX108592 IHC-P Image

ZO-1 antibody detects ZO-1 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
ZO-1 stained by ZO-1 antibody (GTX108592) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ZO-1 antibody used in IHC (Paraffin sections) (IHC-P). GTX108592

GTX108592 IHC-P Image

ZO-1 antibody detects ZO-1 protein at cell membrane and cytoplasm in mouse cervix by immunohistochemical analysis.
Sample: Paraffin-embedded mouse cervix.
ZO-1 antibody (GTX108592) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ZO-1 antibody used in Western Blot (WB). GTX108592

GTX108592 WB Image

Orthogonal

The data was published in the journal Oncotarget in 2016. PMID: 26517522

Anti-ZO-1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX108592

GTX108592 IHC-Fr Image

The data was published in the journal Proc Natl Acad Sci U S A in 2019. PMID: 31064871

文献引用

Application Reference

Chua HH et al. Cell Mol Gastroenterol Hepatol 2024; 17 (3) : 361-381 Antagonism Between Gut Ruminococcus gnavus and Akkermansia muciniphila Modulates the Progression of Chronic Hepatitis B.
Han P et al. Cell Commun Signal 2022; 20 (1) : 149 Cell adhesion molecule BVES functions as a suppressor of tumor cells extrusion in hepatocellular carcinoma metastasis.
实验应用 : WB, ICC/IF
种属反应 : Human
Wei MF et al. Oxid Med Cell Longev 2022; 2022 : 7957255 Atorvastatin Attenuates Radiotherapy-Induced Intestinal Damage through Activation of Autophagy and Antioxidant Effects.
实验应用 : WB, IHC-P
种属反应 : Mouse
Peng-Hsiang Fang et al. Mol Med 2022; 28 (1) : 71 Cobalt protoporphyrin promotes human keratinocyte migration under hyperglycemic conditions.
实验应用 : WB
种属反应 : Human
Tomi E.D. et al. Int J Mol Sci. 2022; 23 (3) : 1647 Upregulation of miR-34a-5p, miR-20a-3p and miR-29a-3p by Onconase in A375 Melanoma Cells Correlates with the Downregulation of Specific Onco-Proteins.
实验应用 : WB
种属反应 : Human
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产品评论

ZO-1 antibody 目录号 GTX108592

Rating
( Average 5 based on 1 users reviews)
实验应用
Immunocytochemistry/ Immunofluorescence(ICC/IF)
( Average 5 based on 1 users reviews)
Anti-ZO-1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108592
Date : Anonymous submitted on 25-Oct-2021
Score :
Application Tested : ICC/IF
Sample Species : Hu
Sample : Caco-2 cells
Blocking : 0.5% skim milk in TBST, RT, 1Hr
Fixation : 4%PFA/PBS, 15 min
Permeabilization /Antigen retrieval : TBST
Primary Antibody : 1:2000, 25°C, 12Hr
我要评论产品
Application Reference
Chua HH et al. Cell Mol Gastroenterol Hepatol 2024; 17 (3):361-381 Antagonism Between Gut Ruminococcus gnavus and Akkermansia muciniphila Modulates the Progression of Chronic Hepatitis B.
Han P et al. Cell Commun Signal 2022; 20 (1):149 Cell adhesion molecule BVES functions as a suppressor of tumor cells extrusion in hepatocellular carcinoma metastasis.
实验应用 : WB, ICC/IF
种属反应 : Human
Wei MF et al. Oxid Med Cell Longev 2022; 2022:7957255 Atorvastatin Attenuates Radiotherapy-Induced Intestinal Damage through Activation of Autophagy and Antioxidant Effects.
实验应用 : WB, IHC-P
种属反应 : Mouse
Peng-Hsiang Fang et al. Mol Med 2022; 28 (1):71 Cobalt protoporphyrin promotes human keratinocyte migration under hyperglycemic conditions.
实验应用 : WB
种属反应 : Human
Tomi E.D. et al. Int J Mol Sci. 2022; 23 (3):1647 Upregulation of miR-34a-5p, miR-20a-3p and miR-29a-3p by Onconase in A375 Melanoma Cells Correlates with the Downregulation of Specific Onco-Proteins.
实验应用 : WB
种属反应 : Human
Wenyi Fang et al. Mol Med Rep 2021; 23 (3):1 Effects of Qing Hua Chang Yin on lipopolysaccharide?induced intestinal epithelial tight junction injury in Caco?2 cells.
实验应用 : WB
种属反应 : Human
Yoshinobu Kariya et al. Commun Biol 2021; 4 (1):490 αvβ3 Integrin induces partial EMT independent of TGF-β signaling.
实验应用 : WB, ICC/IF
种属反应 : Human
Watanabe D et al. Pharmaceutics 2021; 13 (9) Characterization of a Primate Blood-Brain Barrier Co-Culture Model Prepared from Primary Brain Endothelial Cells, Pericytes and Astrocytes.
实验应用 : WB
种属反应 : Monkey
Barbian ME et al. Pediatr Res 2021; (Epub) Butyrate supplementation to pregnant mice elicits cytoprotection against colonic injury in the offspring.
实验应用 : IHC-P
Minamide K et al. Sci Rep 2020; 10 (1):14639 IRF2 maintains the stemness of colonic stem cells by limiting physiological stress from interferon.
实验应用 : IHC-P
种属反应 : Mouse
Bisi S et al. Nat Commun 2020; 11 (1):3516 IRSp53 controls plasma membrane shape and polarized transport at the nascent lumen in epithelial tubules.
Xie MY et al. Biochem Pharmacol 2020; 175:113898 Porcine milk exosome miRNAs protect intestinal epithelial cells against deoxynivalenol-induced damage.
实验应用 : WB
种属反应 : Pig
Kaliannan K et al. Commun Biol 2019; 2:276 Multi-omic analysis in transgenic mice implicates omega-6/omega-3 fatty acid imbalance as a risk factor for chronic disease.
实验应用 : IHC-P
种属反应 : Mouse
Koch F et al. Proc Natl Acad Sci U S A 2019; 116 (21):10333-10338 Heat stress directly impairs gut integrity and recruits distinct immune cell populations into the bovine intestine.
实验应用 : IHC-Fr
种属反应 : Bovine
Li W et al. J Clin Invest 2017; (Epub) The FOXN3-NEAT1-SIN3A repressor complex promotes progression of hormonally responsive breast cancer.
实验应用 : WB
种属反应 : Human
Jo H et al. Food Chem Toxicol 2017; 108 (Pt A):203-213 Oxyresveratrol improves tight junction integrity through the PKC and MAPK signaling pathways in Caco-2 cells.
实验应用 : WB
种属反应 : Human
Sheng-Huei Yang et al. Oncotarget. 2016; 7 (1) Integrin β3 and LKB1 are independently involved in the inhibition of proliferation by lovastatin in human intrahepatic cholangiocarcinoma
实验应用 : WB
种属反应 : Human
Hwang D et al. Biomed Pharmacother 2016; Conditioned medium from LS 174T goblet cells treated with oxyresveratrol strengthens tight junctions in Caco-2 cells.
实验应用 : WB
种属反应 : Human
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
联系经销商
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