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Zika virus Envelope protein抗体 [GT363]

Anti-Zika virus Envelope protein antibody [GT363] used in Western Blot (WB). GTX634155
Anti-Zika virus Envelope protein antibody [GT363] used in ELISA (ELISA). GTX634155
Anti-Zika virus Envelope protein antibody [GT363] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX634155
Anti-Zika virus Envelope protein antibody [GT363] used in ELISA (ELISA). GTX634155
Anti-Zika virus Envelope protein antibody [GT363] used in ELISA (ELISA). GTX634155
Anti-Zika virus Envelope protein antibody [GT363] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX634155
Anti-Zika virus Envelope protein antibody [GT363] used in Western Blot (WB). GTX634155
Anti-Zika virus Envelope protein antibody [GT363] used in ELISA (ELISA). GTX634155
Anti-Zika virus Envelope protein antibody [GT363] used in Western Blot (WB). GTX634155
Anti-Zika virus Envelope protein antibody [GT363] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX634155

目录号 GTX634155

宿主

Mouse

克隆

Monoclonal

克隆编号

GT363

同种型

IgG2b

实验应用

WB, ICC/IF, ELISA, IHC, Sandwich ELISA

种属反应

Zika virus
产品包装
100 μl (¥4000),
25 μl (¥1700)

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:50-1:3000
ICC/IF 1:100-1:1000
ELISA 1:1000-1:10000
IHC Assay dependent
Sandwich ELISA Assay dependent

Note :

Sandwich ELISA
Capture : GTX634155 / GTX133326, Detection : GTX133326 / GTX634155 / GTX133325

WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

54 kDa. ( Note )

阳性对照

Zika Virus-PRVABC59 infected C6/36 cells

产品属性

形式

Liquid

存储溶液

PBS

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Zika virus

免疫原

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of Zika virus Envelope protein (Zika virus (strain H/PF/2013)). The exact sequence is proprietary.

纯化方式

Affinity purified by Protein A.

偶联

Unconjugated

RRID

AB_2888405

注意事项

仅供实验室使用。不适用于人类或动物的任何临床,治疗或诊断用途。不适合动物或人类食用。

相关讯息

產品图片

Anti-Zika virus Envelope protein antibody [GT363] used in Western Blot (WB). GTX634155

GTX634155 WB Image

Zika virus Viral Lysate (0.5 Âμg) was separated by 10% SDS-PAGE, and the membrane was blotted with Zika virus Envelope protein antibody [GT363] (GTX634155) diluted at 1:2000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Zika virus Envelope protein antibody [GT363] used in ELISA (ELISA). GTX634155

GTX634155 ELISA Image

Sandwich ELISA detection of Zika virus-infected lysate using GTX634155 as capture antibody at concentration of 5 μg/mL and GTX133326 as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-Zika virus Envelope protein antibody [GT363] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX634155

GTX634155 ICC/IF Image

Immunofluorescent analysis of arboviruses infected cells using Zika virus Envelope protein antibody [GT363] (GTX634155).
Sample: Mock and zika virus-infected cells.
Green: Zika virus Envelope protein antibody [GT363] (GTX634155) diluted at 1:100.

Anti-Zika virus Envelope protein antibody [GT363] used in ELISA (ELISA). GTX634155

GTX634155 ELISA Image

Sandwich ELISA detection of non-transfected and transfected 293T whole cell extracts using GTX634155 as capture antibody at concentration of 5 μg/mL and GTX133326 as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-Zika virus Envelope protein antibody [GT363] used in ELISA (ELISA). GTX634155

GTX634155 ELISA Image

Sandwich ELISA detection of non-transfected and transfected 293T whole cell extracts using GTX133326 as capture antibody at concentration of 5 μg/mL and GTX634155 as detection antibody at concentration of 1 μg/mL. Mouse IgG antibody (HRP) (GTX213111-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-Zika virus Envelope protein antibody [GT363] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX634155

GTX634155 ICC/IF Image

Immunofluorescent analysis of non-infected and infected vero cells using Zika virus Envelope protein antibody [GT363] (GTX634155).
Green: Zika virus Envelope protein antibody [GT363] (GTX634155) diluted at 1:500.

Anti-Zika virus Envelope protein antibody [GT363] used in Western Blot (WB). GTX634155

GTX634155 WB Image

Non-infected (–) and infected (+) Vero cell extracts (15 μg, unboiled) were separated by gradient gel, and the membrane was blotted with Zika virus Envelope protein antibody [GT363] (GTX634155) culture supernatant diluted at 1:50.

Anti-Zika virus Envelope protein antibody [GT363] used in ELISA (ELISA). GTX634155

GTX634155 ELISA Image

Sandwich ELISA detection of non-transfected and transfected 293T whole cell extracts using GTX634155 as capture antibody at concentration of 5 μg/mL and GTX133325 as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-Zika virus Envelope protein antibody [GT363] used in Western Blot (WB). GTX634155

GTX634155 WB Image

Untreated (–) and treated (+) C6/36 cell extracts (15 μg) were separated by gradient gel, and the membrane was blotted with Zika virus Envelope protein antibody [GT363] (GTX634155) diluted at 1:2000.

Anti-Zika virus Envelope protein antibody [GT363] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX634155

GTX634155 ICC/IF Image

The data was published in the journal Sci Rep in 2017. PMID: 29093521

文献引用

产品评论

Zika virus Envelope protein antibody [GT363] 目录号 GTX634155

Rating ( Average 5 based on 3 users reviews)
实验应用
Western Blot(WB) ( Average 5 based on 2 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 24-Mar-2017
Score :
Application Tested : WB
Sample Species : ZIKV
Sample : vero cells
Amount used : 15 μg
Primary Antibody : 1:50, 4°C, 16Hr
Date : Anonymous submitted on 24-Mar-2017
Score :
Application Tested : WB
Sample Species : ZIKV
Sample : C6/36 cells
Amount used : 15 μg
Primary Antibody : 1:2000, 4°C, 16Hr
Date : Anonymous submitted on 24-Mar-2017
Score :
Application Tested : ICC/IF
Sample Species : ZIKV
Sample : vero cells
Primary Antibody : 1:500, 4°C, 16Hr
SDS
PBS.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
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