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Zika virus Envelope protein抗体

Orthogonal
Anti-Zika virus Envelope protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133325
Anti-Zika virus Envelope protein antibody used in Western Blot (WB). GTX133325
Anti-Zika virus Envelope protein antibody used in Western Blot (WB). GTX133325
Anti-Zika virus Envelope protein antibody used in ELISA (ELISA). GTX133325
Anti-Zika virus Envelope protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133325
Anti-Zika virus Envelope protein antibody used in Western Blot (WB). GTX133325
Anti-Zika virus Envelope protein antibody used in ELISA (ELISA). GTX133325
Anti-Zika virus Envelope protein antibody used in Western Blot (WB). GTX133325

目录号 GTX133325

文献引用 4

宿主

 Rabbit

克隆

 Polyclonal

同种型

 IgG

实验应用

 WB, ICC/IF, ELISA, Sandwich ELISA

种属反应

 Zika virus
产品包装
100 μl (¥4000),
25 μl (¥1700)
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产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
ICC/IF 1:100-1:1000
ELISA 1:1000-1:10000
Sandwich ELISA Assay dependent

Note :

Sandwich ELISA
Capture : GTX634157 / GTX634155, Detection : GTX133325
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

54 kDa. ( Note )

阳性对照

Zika-infected Vero , Zika virus Envelope-transfected 293T , Zika virus Envelope-infected C6/36

产品属性

形式

Liquid

存储溶液

PBS, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1.14 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Zika virus

免疫原

Recombinant protein encompassing a sequence within the center region of Zika virus Envelope protein (Zika virus (strain H/PF/2013)). The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_2886930

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

Zika virus Envelope , ZIKV Envelope

研究领域

產品图片

Anti-Zika virus Envelope protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133325

GTX133325 ICC/IF Image

Immunofluorescent analysis of mock and Zika virus-infected cells using Zika virus Envelope protein antibodyantibody (GTX133325).
Sample: Zika virus non-infected and infected cells slide.
Green: Zika virus Envelope protein antibodyantibody (GTX133325) diluted at 1:100.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Zika virus Envelope protein antibody used in Western Blot (WB). GTX133325

GTX133325 WB Image

Mock and infected Vero whole cell extracts were separated by gradient gel, and the membrane was blotted with Zika virus Envelope protein antibody (GTX133325) diluted at 1:2500.

Anti-Zika virus Envelope protein antibody used in Western Blot (WB). GTX133325

GTX133325 WB Image

Non-infected (–) and infected (+) C6/36 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Zika virus Envelope protein antibody (GTX133325) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Zika virus Envelope protein antibody used in ELISA (ELISA). GTX133325

GTX133325 ELISA Image

Sandwich ELISA detection of non-transfected and transfected 293T whole cell extracts using GTX634157 as capture antibody at concentration of 5 μg/mL and GTX133325 as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-Zika virus Envelope protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133325

GTX133325 ICC/IF Image

Immunofluorescent analysis of non-infected and infected vero cells using Zika virus Envelope protein antibody (GTX133325).
Green: Zika virus Envelope protein antibody (GTX133325) diluted at 1:500.

Anti-Zika virus Envelope protein antibody used in Western Blot (WB). GTX133325

GTX133325 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (60 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Zika virus Envelope protein antibody (GTX133325) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Zika virus Envelope protein antibody used in ELISA (ELISA). GTX133325

GTX133325 ELISA Image

Sandwich ELISA detection of non-transfected and transfected 293T whole cell extracts using GTX634155 as capture antibody at concentration of 5 μg/mL and GTX133325 as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-Zika virus Envelope protein antibody used in Western Blot (WB). GTX133325

GTX133325 WB Image

Orthogonal

The data was published in the journal Sci Rep in 2018. PMID: 29895962

文献引用

Application Reference

Wu H et al. Nat Commun 2023; 14 (1) : 5541 Zika virus targets human trophoblast stem cells and prevents syncytialization in placental trophoblast organoids.
BR Sahoo et al. J Virol 2020; 94 (22) : e01575-20 Mechanistic Target of Rapamycin Signaling Activation Antagonizes Autophagy To Facilitate Zika Virus Replication
实验应用 : WB
Kabir MA et al. Sci Rep 2020; 10 (1) : 11906 An antibody panel for highly specific detection and differentiation of Zika virus.
Fink SL et al. Sci Rep 2018; 8 (1) : 8989 The Antiviral Drug Arbidol Inhibits Zika Virus.
实验应用 : WB
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PBS.pdf
Glycerol.pdf
Proclin.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
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