Non-infected (–) and infected (+) C6/36 whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Zika virus NS4B protein antibody (GTX133321) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Zika virus NS4B protein antibody (GTX133321) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Zika virus NS4B protein antibody detects Zika virus NS4B protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mock and Zika virus NS4B transfected 293T.
Green: Zika virus NS4B stained by Zika virus NS4B protein antibody (GTX133321) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunofluorescent analysis of arboviruses infected cells using Zika virus NS4B protein antibody (GTX133321).
Samples: EUROIMMUN Arboviral Fever Mosaic 2 slide (FR 2668-1010-1).
Green:Zika virus NS4B protein antibody (GTX133321) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Immunofluorescent analysis of mock and Zika virus-infected cells using Zika virus NS4B protein antibodyantibody (GTX133321).
Sample: Zika virus non-infected and infected cells slide.
Green: Zika virus NS4B protein antibodyantibody (GTX133321) diluted at 1:100.
Blue: Fluoroshield with DAPI (GTX30920).

The data was published in the journal Elife in 2018.PMID: 30511641

The data was published in the journal Elife in 2018.PMID: 30511641

The data was published in the journal Elife in 2018.PMID: 30511641