产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:500-1:3000 |
| 1:100-1:1000 |
| Assay dependent |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
阳性对照
NIH-3T3 , 293T , A431 , HeLa , HepG2 , PC-12 , Rat2 , rat brain
预测种属反应
Bovine, Chicken, Pig, Xenopus laevis(>80% identity)
产品属性
形式
Liquid
存储溶液
0.1M Tris, 0.1M Glycine, 10% Glycerol
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
0.09 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant protein encompassing a sequence within the center region of human alpha Actinin 1. The exact sequence is proprietary.
纯化方式
Purified by antigen-affinity chromatography.
偶联
Unconjugated
RRID
AB_1949576
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
actinin alpha 1 , BDPLT15
细胞定位
Cytoplasm , cytoskeleton , myofibril , sarcomere , Z-disk
功能与背景
Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, cytoskeletal, alpha actinin isoform and maps to the same site as the structurally similar erythroid beta spectrin gene. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
数据库
研究领域
產品图片
![Anti-alpha Actinin 1 antibody [N2N3] used in Western Blot (WB). GTX103240](/templates/default/js/lightbox/images/loading.gif "Anti-alpha Actinin 1 antibody [N2N3] used in Western Blot (WB). GTX103240")
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GTX103240 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with alpha Actinin 1 antibody [N2N3] (GTX103240) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX103240 WB Image
Sample (30 μg of whole cell lysate)
A:NIH-3T3
7.5% SDS PAGE
GTX103240 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX103240 WB Image
alpha Actinin 1 antibody detects alpha Actinin 1 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with alpha Actinin 1 antibody (GTX103240) diluted at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX103240 WB Image
Rat tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with alpha Actinin 1 antibody [N2N3] (GTX103240) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX103240 IHC-P Image
Immunohistochemical analysis of paraffin-embedded SW480 xenograft , using Alpha-actinin 1(GTX103240) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX103240 WB Image
Whole zebrafish extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with alpha Actinin 1 antibody [N2N3] (GTX103240) diluted at 1:1000.
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GTX103240 IHC-Wm Image
alpha Actinin 1 antibody [N2N3] detects alpha Actinin 1 protein on zebrafish by whole mount immunohistochemical analysis.
Sample: 2 days-post-fertilization zebrafish embryo.
alpha Actinin 1 antibody [N2N3] (GTX103240) dilution: 1:50.
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文献引用
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产品评论
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![Anti-alpha Actinin 1 antibody [N2N3] used in Western Blot (WB). GTX103240](/upload/website/prouct_img/small/GTX103240/GTX103240_40002_20170901_WB_R_w_23060119_387.webp "Anti-alpha Actinin 1 antibody [N2N3] used in Western Blot (WB). GTX103240")
![Anti-alpha Actinin 1 antibody [N2N3] used in Western Blot (WB). GTX103240](/upload/website/prouct_img/small/GTX103240/GTX103240_WB_M_w_23060119_928.webp "Anti-alpha Actinin 1 antibody [N2N3] used in Western Blot (WB). GTX103240")
![Anti-alpha Actinin 1 antibody [N2N3] used in Western Blot (WB). GTX103240](/upload/website/prouct_img/small/GTX103240/GTX103240_40002_20150327_WB_w_23060119_920.webp "Anti-alpha Actinin 1 antibody [N2N3] used in Western Blot (WB). GTX103240")
![Anti-alpha Actinin 1 antibody [N2N3] used in Western Blot (WB). GTX103240](/upload/website/prouct_img/small/GTX103240/GTX103240_40002_20170901_WB_R_brain_w_23060119_821.webp "Anti-alpha Actinin 1 antibody [N2N3] used in Western Blot (WB). GTX103240")
![Anti-alpha Actinin 1 antibody [N2N3] used in IHC (Paraffin sections) (IHC-P). GTX103240](/upload/website/prouct_img/small/GTX103240/GTX103240_IHC_w_23060119_594.webp "Anti-alpha Actinin 1 antibody [N2N3] used in IHC (Paraffin sections) (IHC-P). GTX103240")
![Anti-alpha Actinin 1 antibody [N2N3] used in Western Blot (WB). GTX103240](/upload/website/prouct_img/small/GTX103240/GTX103240_40002_20160310_WB_Z_22111423_544.webp "Anti-alpha Actinin 1 antibody [N2N3] used in Western Blot (WB). GTX103240")
![Anti-alpha Actinin 1 antibody [N2N3] used in IHC (Whole mount) (IHC-Wm). GTX103240](/upload/website/prouct_img/small/GTX103240/GTX103240_42039_20151008_IHC-Wm_Z_22111423_539.webp "Anti-alpha Actinin 1 antibody [N2N3] used in IHC (Whole mount) (IHC-Wm). GTX103240")
![Anti-alpha Actinin 1 antibody [N2N3] used in Western Blot (WB). GTX103240](/upload/website/prouct_img/normal/GTX103240/GTX103240_40002_20170901_WB_R_w_23060119_387.webp "Anti-alpha Actinin 1 antibody [N2N3] used in Western Blot (WB). GTX103240")
![Anti-alpha Actinin 1 antibody [N2N3] used in Western Blot (WB). GTX103240](/upload/website/prouct_img/normal/GTX103240/GTX103240_WB_M_w_23060119_928.webp "Anti-alpha Actinin 1 antibody [N2N3] used in Western Blot (WB). GTX103240")
![Anti-alpha Actinin 1 antibody [N2N3] used in Western Blot (WB). GTX103240](/upload/website/prouct_img/normal/GTX103240/GTX103240_40002_20150327_WB_w_23060119_920.webp "Anti-alpha Actinin 1 antibody [N2N3] used in Western Blot (WB). GTX103240")
![Anti-alpha Actinin 1 antibody [N2N3] used in Western Blot (WB). GTX103240](/upload/website/prouct_img/normal/GTX103240/GTX103240_40002_20170901_WB_R_brain_w_23060119_821.webp "Anti-alpha Actinin 1 antibody [N2N3] used in Western Blot (WB). GTX103240")
![Anti-alpha Actinin 1 antibody [N2N3] used in IHC (Paraffin sections) (IHC-P). GTX103240](/upload/website/prouct_img/normal/GTX103240/GTX103240_IHC_w_23060119_594.webp "Anti-alpha Actinin 1 antibody [N2N3] used in IHC (Paraffin sections) (IHC-P). GTX103240")
![Anti-alpha Actinin 1 antibody [N2N3] used in Western Blot (WB). GTX103240](/upload/website/prouct_img/normal/GTX103240/GTX103240_40002_20160310_WB_Z_22111423_544.webp "Anti-alpha Actinin 1 antibody [N2N3] used in Western Blot (WB). GTX103240")
![Anti-alpha Actinin 1 antibody [N2N3] used in IHC (Whole mount) (IHC-Wm). GTX103240](/upload/website/prouct_img/normal/GTX103240/GTX103240_42039_20151008_IHC-Wm_Z_22111423_539.webp "Anti-alpha Actinin 1 antibody [N2N3] used in IHC (Whole mount) (IHC-Wm). GTX103240")
