产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
实验应用 |
稀释倍数 |
1:500-1:3000 |
1:100-1:1000 |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
阳性对照
HepG2
产品说明
Based on sequence homology, this antibody may cross react with ACTN1 protein.
预测种属反应
Rat, Drosophila, Bovine, Chicken, Pig, Xenopus tropicalis(>80% identity)
产品属性
形式
Liquid
存储溶液
PBS
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1.27 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant protein encompassing a sequence within the center region of human alpha Actinin 2. The exact sequence is proprietary.
纯化方式
Affinity purified by Protein G.
偶联
Unconjugated
RRID
AB_2888310
注意事项
仅供实验室使用。不适用于人类或动物的任何临床,治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
actinin alpha 2 , CMD1AA , CMH23
细胞定位
Cytoplasm , myofibril , sarcomere , Z-disk
功能与背景
Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a muscle-specific, alpha actinin isoform that is expressed in both skeletal and cardiac muscles. [provided by RefSeq]
数据库
研究领域
產品图片
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GTX632361 IHC-P Image
alpha Actinin 2 antibody [GT1253] detects alpha Actinin 2 protein at cytoplasm in mouse muscle by immunohistochemical analysis. Sample: Paraffin-embedded mouse muscle. alpha Actinin 2 antibody [GT1253] (GTX632361) diluted at 1:250.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX632361 WB Image
Mouse tissue extract (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with alpha Actinin 2 antibody [GT1253] (GTX632361) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX632361 WB Image
alpha Actinin 2 antibody detects alpha Actinin 2 protein by western blot analysis. Whole cell extracts (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with alpha Actinin 2 antibody (GTX632361) at a dilution of 1:1000.
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文献引用
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