产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:500-1:10000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
阳性对照
NIH-3T3 , A431 , H1299 , Rat heart
产品说明
Based on sequence homology, this antibody may cross react with ACTN1 protein.
预测种属反应
Bovine, Chicken, Pig, Xenopus tropicalis(>80% identity)
产品属性
形式
Liquid
存储溶液
0.1M Tris, 0.1M Glycine, 10% Glycerol
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant protein encompassing a sequence within the center region of human alpha Actinin 2. The exact sequence is proprietary.
纯化方式
Purified by antigen-affinity chromatography.
偶联
Unconjugated
RRID
AB_1949577
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
actinin alpha 2 , CMD1AA , CMH23
细胞定位
Cytoplasm , myofibril , sarcomere , Z-disk
功能与背景
Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a muscle-specific, alpha actinin isoform that is expressed in both skeletal and cardiac muscles. [provided by RefSeq]
数据库
研究领域
產品图片
![Anti-alpha Actinin 2 antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX111167](/templates/default/js/lightbox/images/loading.gif "Anti-alpha Actinin 2 antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX111167")
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GTX111167 IHC-P Image
Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using alpha Actinin 2 antibody [N2C1], Internal (GTX111167) at 1:300 dilution.
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GTX111167 WB Image
Sample (10 μg of whole cell lysate)
A: adult zebrafish
7.5% SDS PAGE
GTX111167 diluted at 1:3000
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GTX111167 WB Image
Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
B: H1299
7.5% SDS PAGE
GTX111167 diluted at 1:1000
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GTX111167 WB Image
alpha Actinin 2 antibody detects alpha Actinin 2 protein by western blot analysis. Rat tissue extracts (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with alpha Actinin 2 antibody (GTX111167) diluted at 1:5000.
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GTX111167 ICC/IF Image
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using actinin, alpha 2(GTX111167) antibody at 1:200 dilution.
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GTX111167 IHC-P Image
alpha Actinin 2 antibody [N2C1], Internal detects alpha Actinin 2 protein at cytoplasm and membrane on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
alpha Actinin 2 antibody [N2C1], Internal (GTX111167) diluted at 1:250.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX111167 WB Image
Sample (30 ug of whole cell lysate)
A: NIH-3T3
7.5% SDS PAGE
GTX111167 diluted at 1:3000
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GTX111167 WB Image
Mouse tissue extract (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with alpha Actinin 2 antibody [N2C1], Internal (GTX111167) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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文献引用
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产品评论
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![Anti-alpha Actinin 2 antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX111167](/upload/website/prouct_img/small/GTX111167/GTX111167_40086_IHC_Z_22111423_487.webp "Anti-alpha Actinin 2 antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX111167")
![Anti-alpha Actinin 2 antibody [N2C1], Internal used in Western Blot (WB). GTX111167](/upload/website/prouct_img/small/GTX111167/GTX111167_40086_WB_Z_22111423_153.webp "Anti-alpha Actinin 2 antibody [N2C1], Internal used in Western Blot (WB). GTX111167")
![Anti-alpha Actinin 2 antibody [N2C1], Internal used in Western Blot (WB). GTX111167](/upload/website/prouct_img/small/GTX111167/GTX111167_WB_w_23060500_847.webp "Anti-alpha Actinin 2 antibody [N2C1], Internal used in Western Blot (WB). GTX111167")
![Anti-alpha Actinin 2 antibody [N2C1], Internal used in Western Blot (WB). GTX111167](/upload/website/prouct_img/small/GTX111167/GTX111167_40086_20150903_WB_R_heart_w_23060500_546.webp "Anti-alpha Actinin 2 antibody [N2C1], Internal used in Western Blot (WB). GTX111167")
![Anti-alpha Actinin 2 antibody [N2C1], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111167](/upload/website/prouct_img/small/GTX111167/GTX111167_IFA_w_23060500_354.webp "Anti-alpha Actinin 2 antibody [N2C1], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111167")
![Anti-alpha Actinin 2 antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX111167](/upload/website/prouct_img/small/GTX111167/GTX111167_40086_20141128_IHC_w_23060500_965.webp "Anti-alpha Actinin 2 antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX111167")
![Anti-alpha Actinin 2 antibody [N2C1], Internal used in Western Blot (WB). GTX111167](/upload/website/prouct_img/small/GTX111167/GTX111167_40086_WB_M_w_23060500_334.webp "Anti-alpha Actinin 2 antibody [N2C1], Internal used in Western Blot (WB). GTX111167")
![Anti-alpha Actinin 2 antibody [N2C1], Internal used in Western Blot (WB). GTX111167](/upload/website/prouct_img/small/GTX111167/GTX111167_40086_20220812_WB_M_tissue_22081423_498.webp "Anti-alpha Actinin 2 antibody [N2C1], Internal used in Western Blot (WB). GTX111167")
![Anti-alpha Actinin 2 antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX111167](/upload/website/prouct_img/normal/GTX111167/GTX111167_40086_IHC_Z_22111423_487.webp "Anti-alpha Actinin 2 antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX111167")
![Anti-alpha Actinin 2 antibody [N2C1], Internal used in Western Blot (WB). GTX111167](/upload/website/prouct_img/normal/GTX111167/GTX111167_40086_WB_Z_22111423_153.webp "Anti-alpha Actinin 2 antibody [N2C1], Internal used in Western Blot (WB). GTX111167")
![Anti-alpha Actinin 2 antibody [N2C1], Internal used in Western Blot (WB). GTX111167](/upload/website/prouct_img/normal/GTX111167/GTX111167_WB_w_23060500_847.webp "Anti-alpha Actinin 2 antibody [N2C1], Internal used in Western Blot (WB). GTX111167")
![Anti-alpha Actinin 2 antibody [N2C1], Internal used in Western Blot (WB). GTX111167](/upload/website/prouct_img/normal/GTX111167/GTX111167_40086_20150903_WB_R_heart_w_23060500_546.webp "Anti-alpha Actinin 2 antibody [N2C1], Internal used in Western Blot (WB). GTX111167")
![Anti-alpha Actinin 2 antibody [N2C1], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111167](/upload/website/prouct_img/normal/GTX111167/GTX111167_IFA_w_23060500_354.webp "Anti-alpha Actinin 2 antibody [N2C1], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111167")
![Anti-alpha Actinin 2 antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX111167](/upload/website/prouct_img/normal/GTX111167/GTX111167_40086_20141128_IHC_w_23060500_965.webp "Anti-alpha Actinin 2 antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX111167")
![Anti-alpha Actinin 2 antibody [N2C1], Internal used in Western Blot (WB). GTX111167](/upload/website/prouct_img/normal/GTX111167/GTX111167_40086_WB_M_w_23060500_334.webp "Anti-alpha Actinin 2 antibody [N2C1], Internal used in Western Blot (WB). GTX111167")
![Anti-alpha Actinin 2 antibody [N2C1], Internal used in Western Blot (WB). GTX111167](/upload/website/prouct_img/normal/GTX111167/GTX111167_40086_20220812_WB_M_tissue_22081423_498.webp "Anti-alpha Actinin 2 antibody [N2C1], Internal used in Western Blot (WB). GTX111167")
