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alpha Actinin 4抗体 [C2C3], C-term

Orthogonal
Anti-alpha Actinin 4 antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101669
Anti-alpha Actinin 4 antibody [C2C3], C-term used in Western Blot (WB). GTX101669
Anti-alpha Actinin 4 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX101669
Anti-alpha Actinin 4 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX101669
Anti-alpha Actinin 4 antibody [C2C3], C-term used in Western Blot (WB). GTX101669
Anti-alpha Actinin 4 antibody [C2C3], C-term used in Western Blot (WB). GTX101669
Anti-alpha Actinin 4 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX101669
Anti-alpha Actinin 4 antibody [C2C3], C-term used in Western Blot (WB). GTX101669
Anti-alpha Actinin 4 antibody [C2C3], C-term used in Western Blot (WB). GTX101669

目录号 GTX101669

文献引用 8
1

宿主

 Rabbit

克隆

 Polyclonal

同种型

 IgG

实验应用

 WB, ICC/IF, IHC-P

种属反应

 Human, Mouse, Rat
产品包装
100 μl (¥4000),
25 μl (¥1700)
查看所有 alpha Actinin 4 的产品

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

105 kDa. ( Note )

阳性对照

293T , A431 , HeLa , HepG2 , A375 , Neuro2A , GL261 , C8D30 , NIH-3T3 , BCL-1 , Raw264.7 , C2C12 , Mouse brain , PC-12

预测种属反应

Zebrafish, Bovine, Chicken, Xenopus laevis, Rhesus Monkey(>80% identity)

产品属性

形式

Liquid

存储溶液

0.1M Tris, 0.1M Glycine, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

0.32 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the C-terminus region of human alpha Actinin 4. The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_10617403

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

actinin alpha 4 , ACTININ-4 , FSGS , FSGS1

细胞定位

Nucleus , Cytoplasm

功能与背景

Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, alpha actinin isoform which is concentrated in the cytoplasm, and thought to be involved in metastatic processes. Mutations in this gene have been associated with focal and segmental glomerulosclerosis. [provided by RefSeq]

数据库

  • Gene ID: 81 ACTN4
  • UniProt: O43707 ACTN4

研究领域

產品图片

Anti-alpha Actinin 4 antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101669

GTX101669 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using alpha Actinin 4(GTX101669) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

Anti-alpha Actinin 4 antibody [C2C3], C-term used in Western Blot (WB). GTX101669

GTX101669 WB Image

Sample (50 μg of whole cell lysate)
A: Mouse brain
5% SDS PAGE
GTX101669 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-alpha Actinin 4 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX101669

GTX101669 IHC-P Image

Immunohistochemical analysis of paraffin-embedded A549 xenograft, using alpha Actinin 4(GTX101669) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-alpha Actinin 4 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX101669

GTX101669 IHC-P Image

alpha Actinin 4 antibody [C2C3], C-term detects alpha Actinin 4 protein on mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
alpha Actinin 4 antibody [C2C3], C-term (GTX101669) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-alpha Actinin 4 antibody [C2C3], C-term used in Western Blot (WB). GTX101669

GTX101669 WB Image

alpha Actinin 4 antibody [C2C3], C-term detects ACTN4 protein by western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
7.5% SDS-PAGE
alpha Actinin 4 antibody [C2C3], C-term (GTX101669) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-alpha Actinin 4 antibody [C2C3], C-term used in Western Blot (WB). GTX101669

GTX101669 WB Image

alpha Actinin 4 antibody [C2C3], C-term detects ACTN4 protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg A431 whole cell lysate/extract
C. 30 μg HeLa whole cell lysate/extract
D. 30 μg HepG2 whole cell lysate/extract
E. 30 μg A375 whole cell lysate/extract
7.5% SDS-PAGE
alpha Actinin 4 antibody [C2C3], C-term (GTX101669) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-alpha Actinin 4 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX101669

GTX101669 IHC-P Image

alpha Actinin 4 antibody [C2C3], C-term detects alpha Actinin 4 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
alpha Actinin 4 stained by alpha Actinin 4 antibody [C2C3], C-term (GTX101669) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-alpha Actinin 4 antibody [C2C3], C-term used in Western Blot (WB). GTX101669

GTX101669 WB Image

alpha Actinin 4 antibody [C2C3], C-term detects ACTN4 protein by western blot analysis.
A. 30 μg Neuro2A whole cell lysate/extract
B. 30 μg GL261 whole cell lysate/extract
C. 30 μg C8D30 whole cell lysate/extract
D. 30 μg NIH-3T3 whole cell lysate/extract
E. 30 μg BCL-1 whole cell lysate/extract
F. 30 μg Raw264.7 whole cell lysate/extract
G. 30 μg C2C12 whole cell lysate/extract
7.5% SDS-PAGE
alpha Actinin 4 antibody [C2C3], C-term (GTX101669) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-alpha Actinin 4 antibody [C2C3], C-term used in Western Blot (WB). GTX101669

GTX101669 WB Image

Orthogonal

The data was published in the 2018 in Nat Commun. PMID: 30258067

文献引用

Application Reference

Shu-Hui Yeh et al. Sci Rep 2021; 11 (1) : 15783 Higher senescence associated secretory phenotype and lower defense mediator in urinary extracellular vesicles of elders with and without Parkinson disease.
实验应用 : WB
种属反应 : Human
Ho CH et al. Front Physiol 2021; 12 : 725172 α-Actinin 4 Links Vasopressin Short-Term and Long-Term Regulation of Aquaporin-2 in Kidney Collecting Duct Cells.
实验应用 : WB
Hiraga C et al. Sci Rep 2021; 11 (1) : 3326 Pentapartite fractionation of particles in oral fluids by differential centrifugation.
实验应用 : WB
Takov K et al. J Extracell Vesicles 2019; 8 (1) : 1560809 Comparison of small extracellular vesicles isolated from plasma by ultracentrifugation or size-exclusion chromatography: yield, purity and functional potential.
实验应用 : WB
种属反应 : Rat
Ageta H et al. Nat Commun 2018; 9 (1) : 3936 UBL3 modification influences protein sorting to small extracellular vesicles.
实验应用 : WB
种属反应 : Mouse
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产品评论

alpha Actinin 4 antibody [C2C3], C-term 目录号 GTX101669

Rating
( Average 4 based on 1 users reviews)
实验应用
Western Blot(WB)
( Average 4 based on 1 users reviews)
Anti-alpha Actinin 4 antibody [C2C3], C-term used in Western Blot (WB). GTX101669
Date : Anonymous submitted on 23-Jan-2015
Score :
Application Tested : WB
Sample Species : Hu
Sample : HepG2 cells
Amount used : 30μg
Blocking : 5% milk in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
我要评论产品
Application Reference
Shu-Hui Yeh et al. Sci Rep 2021; 11 (1):15783 Higher senescence associated secretory phenotype and lower defense mediator in urinary extracellular vesicles of elders with and without Parkinson disease.
实验应用 : WB
种属反应 : Human
Ho CH et al. Front Physiol 2021; 12:725172 α-Actinin 4 Links Vasopressin Short-Term and Long-Term Regulation of Aquaporin-2 in Kidney Collecting Duct Cells.
实验应用 : WB
Hiraga C et al. Sci Rep 2021; 11 (1):3326 Pentapartite fractionation of particles in oral fluids by differential centrifugation.
实验应用 : WB
Takov K et al. J Extracell Vesicles 2019; 8 (1):1560809 Comparison of small extracellular vesicles isolated from plasma by ultracentrifugation or size-exclusion chromatography: yield, purity and functional potential.
实验应用 : WB
种属反应 : Rat
Ageta H et al. Nat Commun 2018; 9 (1):3936 UBL3 modification influences protein sorting to small extracellular vesicles.
实验应用 : WB
种属反应 : Mouse
Durrieu L et al. Blood Adv 2018; 2 (10):1054-1065 Analysis of the thrombotic and fibrinolytic activities of tumor cell-derived extracellular vesicles.
种属反应 : Human
Kowal J et al. Proc Natl Acad Sci U S A 2016; 113 (8):E968-77 Proteomic comparison defines novel markers to characterize heterogeneous populations of extracellular vesicle subtypes.
实验应用 : WB
种属反应 : Human
Choi W et al. J Cell Biol 2016; 213 (2):243-60 Remodeling the zonula adherens in response to tension and the role of afadin in this response.
SDS
Glycerol.pdf
Thimerosal.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
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