产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:500-1:3000 |
| 1:100-1:1000 |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
阳性对照
A431 , NIH-3T3 , JC , PC-12 , 293T , A431 , HeLa , HepG2
预测种属反应
Zebrafish, Drosophila, Bovine, Chicken, Xenopus tropicalis, Rhesus Monkey(>80% identity)
产品属性
形式
Liquid
存储溶液
PBS, 1% BSA, 20% Glycerol
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant protein encompassing a sequence within the N-terminus region of human alpha Actinin 4. The exact sequence is proprietary.
纯化方式
Purified by antigen-affinity chromatography.
偶联
Unconjugated
RRID
AB_11173447
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
actinin alpha 4 , ACTININ-4 , FSGS , FSGS1
细胞定位
Nucleus , Cytoplasm
功能与背景
Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, alpha actinin isoform which is concentrated in the cytoplasm, and thought to be involved in metastatic processes. Mutations in this gene have been associated with focal and segmental glomerulosclerosis. [provided by RefSeq]
数据库
研究领域
產品图片
![Anti-alpha Actinin 4 antibody [N1N2], N-term used in Western Blot (WB). GTX113116](/templates/default/js/lightbox/images/loading.gif "Anti-alpha Actinin 4 antibody [N1N2], N-term used in Western Blot (WB). GTX113116")
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GTX113116 WB Image
alpha Actinin 4 antibody detects alpha Actinin 4 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with alpha Actinin 4 antibody (GTX113116) diluted by 1:1000.
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GTX113116 WB Image
Sample (30 ug of whole cell lysate)
A: NIH-3T3
B: JC
7.5% SDS PAGE
GTX113116 diluted at 1:1000
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GTX113116 WB Image
alpha Actinin 4 antibody [N1N2], N-term detects ACTN4 protein by Western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
7.5 % SDS-PAGE
alpha Actinin 4 antibody [N1N2], N-term (GTX113116) dilution: 1:1000
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GTX113116 WB Image
Sample (30 ug of whole cell lysate)
A: A431
7.5% SDS PAGE
GTX113116 diluted at 1:1000
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GTX113116 ICC/IF Image
alpha Actinin 4 antibody [N1N2], N-term detects ACTN4 protein at cytoplasm by immunofluorescent analysis.
Sample: MCF-7 cells were fixed in ice-cold MeOH for 5 min.
Green: ACTN4 protein stained by alpha Actinin 4 antibody [N1N2], N-term (GTX113116) diluted at 1:500.
Blue: Hoechst 33342 staining.
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文献引用
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产品评论
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![Anti-alpha Actinin 4 antibody [N1N2], N-term used in Western Blot (WB). GTX113116](/upload/website/prouct_img/small/GTX113116/GTX113116_40499_WB_2_w_23060500_165.webp "Anti-alpha Actinin 4 antibody [N1N2], N-term used in Western Blot (WB). GTX113116")
![Anti-alpha Actinin 4 antibody [N1N2], N-term used in Western Blot (WB). GTX113116](/upload/website/prouct_img/small/GTX113116/GTX113116_40499_WB_M_w_23060500_134.webp "Anti-alpha Actinin 4 antibody [N1N2], N-term used in Western Blot (WB). GTX113116")
![Anti-alpha Actinin 4 antibody [N1N2], N-term used in Western Blot (WB). GTX113116](/upload/website/prouct_img/small/GTX113116/GTX113116_40499_WB_R_w_23060500_370.webp "Anti-alpha Actinin 4 antibody [N1N2], N-term used in Western Blot (WB). GTX113116")
![Anti-alpha Actinin 4 antibody [N1N2], N-term used in Western Blot (WB). GTX113116](/upload/website/prouct_img/small/GTX113116/GTX113116_40499_WB_w_23060500_994.webp "Anti-alpha Actinin 4 antibody [N1N2], N-term used in Western Blot (WB). GTX113116")
![Anti-alpha Actinin 4 antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113116](/upload/website/prouct_img/small/GTX113116/GTX113116_40499_IFA_w_23060500_629.webp "Anti-alpha Actinin 4 antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113116")
![Anti-alpha Actinin 4 antibody [N1N2], N-term used in Western Blot (WB). GTX113116](/upload/website/prouct_img/normal/GTX113116/GTX113116_40499_WB_2_w_23060500_165.webp "Anti-alpha Actinin 4 antibody [N1N2], N-term used in Western Blot (WB). GTX113116")
![Anti-alpha Actinin 4 antibody [N1N2], N-term used in Western Blot (WB). GTX113116](/upload/website/prouct_img/normal/GTX113116/GTX113116_40499_WB_M_w_23060500_134.webp "Anti-alpha Actinin 4 antibody [N1N2], N-term used in Western Blot (WB). GTX113116")
![Anti-alpha Actinin 4 antibody [N1N2], N-term used in Western Blot (WB). GTX113116](/upload/website/prouct_img/normal/GTX113116/GTX113116_40499_WB_R_w_23060500_370.webp "Anti-alpha Actinin 4 antibody [N1N2], N-term used in Western Blot (WB). GTX113116")
![Anti-alpha Actinin 4 antibody [N1N2], N-term used in Western Blot (WB). GTX113116](/upload/website/prouct_img/normal/GTX113116/GTX113116_40499_WB_w_23060500_994.webp "Anti-alpha Actinin 4 antibody [N1N2], N-term used in Western Blot (WB). GTX113116")
![Anti-alpha Actinin 4 antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113116](/upload/website/prouct_img/normal/GTX113116/GTX113116_40499_IFA_w_23060500_629.webp "Anti-alpha Actinin 4 antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113116")
