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alpha Smooth Muscle Actin抗体 [4A4]

Anti-alpha Smooth Muscle Actin antibody [4A4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60466
Anti-alpha Smooth Muscle Actin antibody [4A4] used in ELISA (ELISA). GTX60466
Anti-alpha Smooth Muscle Actin antibody [4A4] used in Flow cytometry (FACS). GTX60466
Anti-alpha Smooth Muscle Actin antibody [4A4] used in Western Blot (WB). GTX60466
Anti-alpha Smooth Muscle Actin antibody [4A4] used in IHC (Paraffin sections) (IHC-P). GTX60466

目录号 GTX60466

文献引用 2

宿主

 Mouse

克隆

 Monoclonal

克隆编号

 4A4

同种型

 IgG1

实验应用

 WB, ICC/IF, IHC-P, FACS, ELISA

种属反应

 Human, Mouse, Rat, Monkey
产品包装
100 μl (¥5600)
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产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1/500 - 1/2000
ICC/IF 1/200 - 1/1000
IHC-P 1/200 - 1/1000
FACS 1/200 - 1/400
ELISA 1/10000
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

42 kDa. ( Note )

产品属性

形式

Liquid

存储溶液

Ascites

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

免疫原种属

Human

免疫原

Synthesized peptide of human alpha Smooth Muscle Actin.

纯化方式

Unpurified

偶联

Unconjugated

RRID

AB_2887970

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

actin alpha 2, smooth muscle , ACTSA

细胞定位

Cytoplasm, cytoskeleton

功能与背景

The protein encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Defects in this gene cause aortic aneurysm familial thoracic type 6. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Nov 2008]

数据库

  • Gene ID: 59 ACTA2
  • UniProt: P62736 ACTA2

研究领域

產品图片

Anti-alpha Smooth Muscle Actin antibody [4A4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60466

GTX60466 ICC/IF Image

ICC/IF analysis of HepG2 cells using GTX60466 alpha Smooth Muscle Actin antibody [4A4].
Green : alpha Smooth Muscle Actin
Blue: DRAQ5 fluorescent DNA dye
Red: Actin filaments

Anti-alpha Smooth Muscle Actin antibody [4A4] used in ELISA (ELISA). GTX60466

GTX60466 ELISA Image

ELISA analysis of antigen using GTX60466 alpha Smooth Muscle Actin antibody [4A4].
Red : Control antigen 100ng
Purple : Antigen 10ng
Green : Antigen 50ng
Blue : Antigen 100ng

Anti-alpha Smooth Muscle Actin antibody [4A4] used in Flow cytometry (FACS). GTX60466

GTX60466 FACS Image

FACS analysis of HeLa cells using GTX60466 alpha Smooth Muscle Actin antibody [4A4].
Green : alpha Smooth Muscle Actin
Purple : negative control

Anti-alpha Smooth Muscle Actin antibody [4A4] used in Western Blot (WB). GTX60466

GTX60466 WB Image

WB analysis of HeLa (1), A431 (2), Jurkat (3), K562 (4), HEK293 (5), HepG2 (6), NIH3T3 (7), PC-12 (8) and Cos7 (9) cell lysate using GTX60466 alpha Smooth Muscle Actin antibody [4A4].

Anti-alpha Smooth Muscle Actin antibody [4A4] used in IHC (Paraffin sections) (IHC-P). GTX60466

GTX60466 IHC-P Image

IHC-P analysis of human duodenum tissue (left) and human esophagus tissue (right) using GTX60466 alpha Smooth Muscle Actin antibody [4A4].

文献引用

Application Reference

Li CJ et al. Front Cell Dev Biol 2023; 11 : 1331584 Generation of induced pluripotent stem cells from Bornean orangutans.
Nguyen QH et al. Nat Commun 2018; 9 (1) : 2028 Profiling human breast epithelial cells using single cell RNA sequencing identifies cell diversity.
实验应用 : IHC-P
种属反应 : Human
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产品包装 报价 (¥)
¥ 5600
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