Various tissue extracts (50 μg) were separated by 15% SDS-PAGE, and the membranes were blotted with alpha Synuclein antibody [HL1242] (GTX636641) diluted at 1:1000 and competitor's antibody (Competitor) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

alpha Synuclein antibody [HL1242] detects alpha Synuclein protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse cerebellum.
Green: alpha Synuclein stained by alpha Synuclein antibody [HL1242] (GTX636641) diluted at 1:200.
Red: NF-H, a neural marker, stained by NF-H antibody [GT114] (GTX634289) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Various tissue extracts (50 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with alpha Synuclein antibody [HL1242] (GTX636641) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with alpha Synuclein antibody [HL1242] (GTX636641) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

alpha Synuclein antibody [HL1242] detects alpha Synuclein protein by immunofluorescent analysis.
Sample: SH-SY-5Y cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: alpha Synuclein stained by alpha Synuclein antibody [HL1242] (GTX636641) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10μm.

alpha Synuclein antibody [HL1242] detects alpha Synuclein protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissues.
alpha Synuclein stained by alpha Synuclein antibody [HL1242] (GTX636641) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

alpha Synuclein antibody [HL1242] detects alpha Synuclein protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
alpha Synuclein stained by alpha Synuclein antibody [HL1242] (GTX636641) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

alpha Synuclein antibody [HL1242] detects alpha Synuclein protein by immunohistochemical analysis.
Sample: Frozen-sectioned mouse cerebellum.
Green: alpha Synuclein stained by alpha Synuclein antibody [HL1242] (GTX636641) diluted at 1:100.