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alpha Tubulin抗体 [B-5-1-2]

Anti-alpha Tubulin antibody [B-5-1-2] used in Western Blot (WB). GTX11304
Anti-alpha Tubulin antibody [B-5-1-2] used in Western Blot (WB). GTX11304
Anti-alpha Tubulin antibody [B-5-1-2] used in Western Blot (WB). GTX11304

目录号 GTX11304

文献引用 7

宿主

 Mouse

克隆

 Monoclonal

克隆编号

 B-5-1-2

同种型

 IgG1

实验应用

 WB, ICC/IF, IP

种属反应

 Human, Mouse, Rat, Zebrafish, Bovine, Dog, Chicken, Monkey, African green monkey, Kangaroo rat, Sea Urchin, Chlamydomonas
产品包装
100 μl (¥6300)
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产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 0.25-0.5 μg/ml
ICC/IF 0.5-1 μg/ml
IP Assay dependent
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

50 kDa. ( Note )

阳性对照

chicken fibroblast, FS11

产品说明

Recognizes an epitope located at the C-terminal end of the α-tubulin isoform in a variety of organisms.

产品属性

形式

Liquid

存储溶液

PBS

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

~2? mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Sea Urchin

免疫原

Sarkosyl-resistant filaments from Strongylocentrotus purpuratus (sea urchin) sperm axonemes.

纯化方式

Purified immunoglobulin

偶联

Unconjugated

RRID

AB_373242

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

tubulin alpha 4a , ALS22 , H2-ALPHA , TUBA1

细胞定位

Cytoplasm, cytoskeleton

功能与背景

Tubulin is the major building block of microtubules. This intracellular cylindrical filamentous structure is present in almost all eukaryotic cells. Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement and in the cytoskeleton. Tubulin is a heterodimer which consists of alpha tubulin and beta tubulin.

数据库

研究领域

產品图片

Anti-alpha Tubulin antibody [B-5-1-2] used in Western Blot (WB). GTX11304

GTX11304 WB Image

WB analysis of (1) HeLa, (2) Jurkat, (3) COS7, (4) NIH-3T3, (5) PC-12, (6) RAT2, (7) CHO, (8) MDBK and (9) MDCK lysates using alpha Tubulin antibody [B-5-1-2] at 0.5 μg/ml.

Anti-alpha Tubulin antibody [B-5-1-2] used in Western Blot (WB). GTX11304

GTX11304 WB Image

The data was published in the journal Elife in 2015. PMID: 26076409

Anti-alpha Tubulin antibody [B-5-1-2] used in Western Blot (WB). GTX11304

GTX11304 WB Image

The data was published in the journal Elife in 2015. PMID: 26076409

文献引用

Application Reference

Auer TO et al. Elife 2015; 4 (Epub) Deletion of a kinesin I motor unmasks a mechanism of homeostatic branching control by neurotrophin-3.
实验应用 : WB
种属反应 : Zebrafish
De Domenico I et al. Cell Metab 2011; 14 (5) : 635-46 The role of ubiquitination in hepcidin-independent and hepcidin-dependent degradation of ferroportin.
Huang AM et al. Biochem Pharmacol 2011; 82 (1) : 35-42 ube2m-mediated p27(kip1) degradation in * cytotoxicity.
实验应用 : WB
种属反应 : Human
De Domenico I et al. Proc Natl Acad Sci U S A 2009; 106 (10) : 694083 Hepcidin-induced internalization of ferroportin requires binding and cooperative interaction with Jak2.
实验应用 : WB
种属反应 : Human
Paradkar PN et al. Mol Cell Biol 2009; 29 (4) : 1007-16 Regulation of mitochondrial iron import through differential turnover of mitoferrin 1 and mitoferrin 2.
实验应用 : WB
种属反应 : Mouse
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产品包装 报价 (¥)
¥ 6300
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