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alpha Tubulin抗体

Anti-alpha Tubulin antibody used in IHC (Whole mount) (IHC-Wm). GTX102079
Anti-alpha Tubulin antibody used in Western Blot (WB). GTX102079
Anti-alpha Tubulin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102079
Anti-alpha Tubulin antibody used in Western Blot (WB). GTX102079
Anti-alpha Tubulin antibody used in IHC (Paraffin sections) (IHC-P). GTX102079
Anti-alpha Tubulin antibody used in Western Blot (WB). GTX102079

目录号 GTX102079

宿主

Rabbit

克隆

Polyclonal

同种型

IgG

实验应用

WB, ICC/IF, IHC-P, IHC-Wm

种属反应

Human, Zebrafish, Drosophila
产品包装
100 μl (¥4000),
25 μl (¥1700)

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:5000-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Wm Assay dependent
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

50 kDa. ( Note )

阳性对照

293T , A431 , HeLa , HepG2

预测种属反应

Mouse, Rat, Rabbit, Bovine, Pig, Xenopus laevis, Chimpanzee, Rhesus Monkey(>80% identity)

产品属性

形式

Liquid

存储溶液

0.1M Tris, 0.1M Glycine, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

0.63 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the center region of human alpha Tubulin. The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_11163195

注意事项

仅供实验室使用。不适用于人类或动物的任何临床,治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

K-ALPHA-1 antibody , TUBA1B antibody , alpha-tubulin ubiquitous antibody , alpha tubulin antibody , tubulin K-alpha-1 antibody , tubulin alpha-ubiquitous chain antibody , "tubulin , alpha , ubiquitous antibody" , tubulin alpha-1B chain antibody , "tubulin , alpha 1b antibody"

功能与背景

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.

研究领域

產品图片

Anti-alpha Tubulin antibody used in IHC (Whole mount) (IHC-Wm). GTX102079

GTX102079 IHC-Wm Image

alpha Tubulin antibody detects alpha Tubulin protein on whole-mount zebrafish embryos by immunohistochemical analysis.
Sample: Paraformaldehyde-fixed zebrafish embryos.
alpha Tubulin antibody (GTX102079) dilution: 1:200.

Anti-alpha Tubulin antibody used in Western Blot (WB). GTX102079

GTX102079 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin antibody (GTX102079) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Drosophila alphaTub85E is the homolog of mammalian alpha Tubulin. (Gene ID: 41183/ UniProt: P06604)

Anti-alpha Tubulin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102079

GTX102079 ICC/IF Image

alpha Tubulin antibody detects TUBA1B protein at cytoskeleton by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: TUBA1B protein stained by alpha Tubulin antibody (GTX102079) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-alpha Tubulin antibody used in Western Blot (WB). GTX102079

GTX102079 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
B: A431
C: HeLa
D: HepG2
10% SDS PAGE
GTX102079 diluted at 1:10000

Anti-alpha Tubulin antibody used in IHC (Paraffin sections) (IHC-P). GTX102079

GTX102079 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using alpha Tubulin(GTX102079) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-alpha Tubulin antibody used in Western Blot (WB). GTX102079

GTX102079 WB Image

The data was published in the journal PLoS One in 2017. PMID: 28301550

文献引用

产品评论

alpha Tubulin antibody 目录号 GTX102079

Rating ( Average 4 based on 1 users reviews)
实验应用
Western Blot(WB) ( Average 4 based on 1 users reviews)
Date : Anonymous submitted on 28-Dec-2015
Score :
Application Tested : WB
Sample Species : Hu
Sample : primary culture endothelial cells
Amount used : 5μg
Blocking : 3% BSA in TBST, 23°C, 2Hr
Primary Antibody : 1:2000, 25°C, 2Hr
SDS
Glycerol.pdf
Thimerosal.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
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