产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
实验应用 |
稀释倍数 |
1:5000-1:20000 |
1:100-1:1000 |
1:100-1:1000 |
Assay dependent |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
阳性对照
293T , A431 , HeLa , HepG2
预测种属反应
Mouse, Rat, Rabbit, Bovine, Pig, Xenopus laevis, Chimpanzee, Rhesus Monkey(>80% identity)
产品属性
形式
Liquid
存储溶液
0.1M Tris, 0.1M Glycine, 20% Glycerol
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
0.63 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant protein encompassing a sequence within the center region of human alpha Tubulin. The exact sequence is proprietary.
纯化方式
Purified by antigen-affinity chromatography.
偶联
Unconjugated
RRID
AB_11163195
注意事项
仅供实验室使用。不适用于人类或动物的任何临床,治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
K-ALPHA-1 antibody , TUBA1B antibody , alpha-tubulin ubiquitous antibody , alpha tubulin antibody , tubulin K-alpha-1 antibody , tubulin alpha-ubiquitous chain antibody , "tubulin , alpha , ubiquitous antibody" , tubulin alpha-1B chain antibody , "tubulin , alpha 1b antibody"
功能与背景
Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.
研究领域
產品图片
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GTX102079 IHC-Wm Image
alpha Tubulin antibody detects alpha Tubulin protein on whole-mount zebrafish embryos by immunohistochemical analysis. Sample: Paraformaldehyde-fixed zebrafish embryos. alpha Tubulin antibody (GTX102079) dilution: 1:200.
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GTX102079 WB Image
Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin antibody (GTX102079) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Drosophila alphaTub85E is the homolog of mammalian alpha Tubulin. (Gene ID: 41183/ UniProt: P06604)
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GTX102079 ICC/IF Image
alpha Tubulin antibody detects TUBA1B protein at cytoskeleton by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: TUBA1B protein stained by alpha Tubulin antibody (GTX102079) diluted at 1:500. Blue: Hoechst 33342 staining.
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GTX102079 WB Image
Sample (30 ug of whole cell lysate) A: 293T B: A431 C: HeLa D: HepG2 10% SDS PAGE GTX102079 diluted at 1:10000
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GTX102079 IHC-P Image
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using alpha Tubulin(GTX102079) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX102079 WB Image
The data was published in the journal PLoS One in 2017. PMID: 28301550
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文献引用
产品评论
alpha Tubulin antibody
目录号 GTX102079
Rating
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( Average 4 based on 1 users reviews)
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实验应用
Western Blot(WB)
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( Average 4 based on 1 users reviews)
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Date :
Anonymous submitted on 28-Dec-2015
Application Tested :
WB
Sample Species :
Hu
Sample :
primary culture endothelial cells
Amount used :
5μg
Blocking :
3% BSA in TBST, 23°C, 2Hr
Primary Antibody :
1:2000, 25°C, 2Hr