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alpha Tubulin抗体

Anti-alpha Tubulin antibody used in Western Blot (WB). GTX108784
Anti-alpha Tubulin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108784
Anti-alpha Tubulin antibody used in Western Blot (WB). GTX108784
Anti-alpha Tubulin antibody used in IHC (Paraffin sections) (IHC-P). GTX108784

目录号 GTX108784

文献引用 6

宿主

 Rabbit

克隆

 Polyclonal

同种型

 IgG

实验应用

 WB, ICC/IF, IHC-P

种属反应

 Human, Mouse, Rat
产品包装
100 μl (¥4000),
25 μl (¥1700)
查看所有 alpha Tubulin 的产品

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

阳性对照

NIH-3T3 , 293T , A431 , HeLa , HepG2

预测种属反应

Zebrafish, Rabbit, Bovine, Pig, Xenopus laevis, Chimpanzee, Rhesus Monkey(>80% identity)

产品属性

形式

Liquid

存储溶液

0.1M Tris, 0.1M Glycine, 10% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

0.81 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the center region of human alpha Tubulin. The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_1952428

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

研究领域

產品图片

Anti-alpha Tubulin antibody used in Western Blot (WB). GTX108784

GTX108784 WB Image

alpha Tubulin antibody detects alpha Tubulin protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin antibody (GTX108784) diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-alpha Tubulin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108784

GTX108784 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed A431, using Tubulin alpha-1B(GTX108784) antibody at 1:200 dilution.

Anti-alpha Tubulin antibody used in Western Blot (WB). GTX108784

GTX108784 WB Image

Sample (30 μg of whole cell lysate)
A: NIH-3T3
10% SDS PAGE
GTX108784 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-alpha Tubulin antibody used in IHC (Paraffin sections) (IHC-P). GTX108784

GTX108784 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using alpha Tubulin(GTX108784) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

文献引用

Application Reference

Shimada M et al. Nutrition Research 2017; (Epub) Dietary supplementation with myo-inositol reduces hepatic triglyceride accumulation and expression of both fructolytic and lipogenic genes in rats fed a high-fructose diet.
实验应用 : WB
种属反应 : Rat
Zuppinger C et al. EJH 2017; 61 : 2 Characterization of cytoskeleton features and maturation status of cultured human iPSC-derived cardiomyocytes.
Spur EM et al. Toxicology 2016; 353-354 : 34-47 inhibition of chymotryptic-like standard proteasome activity exacerbates *-induced cytotoxicity in primary cardiomyocytes.
实验应用 : WB
种属反应 : Mouse
Ho YC et al. Sci Rep 2016; 6 : 25374 A Novel Protective Function of 5-Methoxytryptophan in Vascular Injury.
实验应用 : WB
种属反应 : Human
Jayachandran P et al. Neural Dev 2016; 11 (1) : 1 Microtubule-associated protein 1b is required for shaping the neural tube.
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SDS
Glycerol.pdf
Thimerosal.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
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