产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:5000-1:50000 |
| Assay dependent |
| Assay dependent |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
预测种属反应
Sheep, Bovine, Chicken, Guinea pig, Xenopus tropicalis, Chimpanzee, Rhesus Monkey(>80% identity)
产品属性
形式
Liquid
存储溶液
PBS
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant fragment of human beta Actin
纯化方式
Affinity purified by Protein A.
偶联
Unconjugated
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
actin beta , BRWS1 , PS1TP5BP1
细胞定位
Cytoplasm, cytoskeleton,Nucleus
功能与背景
This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, integrity, and intercellular signaling. The encoded protein is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins that are ubiquitously expressed. Mutations in this gene cause Baraitser-Winter syndrome 1, which is characterized by intellectual disability with a distinctive facial appearance in human patients. Numerous pseudogenes of this gene have been identified throughout the human genome. [provided by RefSeq, Aug 2017]
数据库
研究领域
產品图片
![Anti-beta Actin antibody [HL1926] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637675](/templates/default/js/lightbox/images/loading.gif "Anti-beta Actin antibody [HL1926] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637675")
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GTX637675 ICC/IF Image
beta Actin antibody [HL1926] detects beta Actin protein by immunofluorescent analysis.Sample: HeLa cells were fixed in ice-cold MeOH for 10 min.Green: beta Actin stained by beta Actin antibody [HL1926] (GTX637675) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
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GTX637675 WB Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Actin antibody [HL1926] (GTX637675) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX637675 WB Image
Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with beta Actin antibody [HL1926] (GTX637675) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX637675 WB Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Actin antibody [HL1926] (GTX637675) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX637675 IHC-P Image
beta Actin antibody [HL1926] detects beta Actin protein at cell membrane by immunohistochemical analysis.Sample: Paraffin-embedded mouse intestine.beta Actin stained by beta Actin antibody [HL1926] (GTX637675) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX637675 WB Image
Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with beta Actin antibody [HL1926] (GTX637675) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
|
|
|
GTX637675 WB Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with beta Actin antibody [HL1926] (GTX637675) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX637675 WB Image
Various whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with beta Actin antibody [HL1926] (GTX637675) diluted at 1:100000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
|
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|
GTX637675 WB Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Actin antibody [HL1926] (GTX637675) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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文献引用
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产品评论
beta Actin antibody [HL1926]
目录号 GTX637675
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Rating
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( Average 5 based on 1 users reviews)
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实验应用
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Western Blot(WB)
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( Average 5 based on 1 users reviews)
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Date :
Anonymous submitted on 20-Dec-2023
Application Tested :
WB
Sample Species :
Ms
Sample :
mouse tissue colon tissue lysates
Amount used :
20μg
Blocking :
5% non-fat milk in TBST, 25°C, 1Hr
Primary Antibody :
1:10000, 4°C, 16Hr
![Anti-beta Actin antibody [HL1926] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637675](/upload/website/prouct_img/small/GTX637675/GTX637675_44900_20221230_ICC_IF_22122901_487.webp "Anti-beta Actin antibody [HL1926] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637675")
![Anti-beta Actin antibody [HL1926] used in Western Blot (WB). GTX637675](/upload/website/prouct_img/small/GTX637675/GTX637675_44900_20230915_WB_23091901_695.webp "Anti-beta Actin antibody [HL1926] used in Western Blot (WB). GTX637675")
![Anti-beta Actin antibody [HL1926] used in Western Blot (WB). GTX637675](/upload/website/prouct_img/small/GTX637675/GTX637675_T-44837_20221111_WB_Drosophila_22111518_843.webp "Anti-beta Actin antibody [HL1926] used in Western Blot (WB). GTX637675")
![Anti-beta Actin antibody [HL1926] used in Western Blot (WB). GTX637675](/upload/website/prouct_img/small/GTX637675/GTX637675_T-44837_20221111_WB_C_D_22111518_626.webp "Anti-beta Actin antibody [HL1926] used in Western Blot (WB). GTX637675")
![Anti-beta Actin antibody [HL1926] used in IHC (Paraffin sections) (IHC-P). GTX637675](/upload/website/prouct_img/small/GTX637675/GTX637675_T-44837_20221111_IHC-P_M_22111518_644.webp "Anti-beta Actin antibody [HL1926] used in IHC (Paraffin sections) (IHC-P). GTX637675")
![Anti-beta Actin antibody [HL1926] used in Western Blot (WB). GTX637675](/upload/website/prouct_img/small/GTX637675/GTX637675_T-44837_20221111_WB_Z_22111518_555.webp "Anti-beta Actin antibody [HL1926] used in Western Blot (WB). GTX637675")
![Anti-beta Actin antibody [HL1926] used in Western Blot (WB). GTX637675](/upload/website/prouct_img/small/GTX637675/GTX637675_45180_20231110_WB_multiple_species_23112021_692.webp "Anti-beta Actin antibody [HL1926] used in Western Blot (WB). GTX637675")
![Anti-beta Actin antibody [HL1926] used in Western Blot (WB). GTX637675](/upload/website/prouct_img/small/GTX637675/GTX637675_45257_20231215_WB_Sensitivity_24012217_356.webp "Anti-beta Actin antibody [HL1926] used in Western Blot (WB). GTX637675")
![Anti-beta Actin antibody [HL1926] used in Western Blot (WB). GTX637675](/upload/website/prouct_img/small/GTX637675/GTX637675_T-44837_20221104_WB_M_R_22110919_481.webp "Anti-beta Actin antibody [HL1926] used in Western Blot (WB). GTX637675")
![Anti-beta Actin antibody [HL1926] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637675](/upload/website/prouct_img/normal/GTX637675/GTX637675_44900_20221230_ICC_IF_22122901_487.webp "Anti-beta Actin antibody [HL1926] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637675")
![Anti-beta Actin antibody [HL1926] used in Western Blot (WB). GTX637675](/upload/website/prouct_img/normal/GTX637675/GTX637675_44900_20230915_WB_23091901_695.webp "Anti-beta Actin antibody [HL1926] used in Western Blot (WB). GTX637675")
![Anti-beta Actin antibody [HL1926] used in Western Blot (WB). GTX637675](/upload/website/prouct_img/normal/GTX637675/GTX637675_T-44837_20221111_WB_Drosophila_22111518_843.webp "Anti-beta Actin antibody [HL1926] used in Western Blot (WB). GTX637675")
![Anti-beta Actin antibody [HL1926] used in Western Blot (WB). GTX637675](/upload/website/prouct_img/normal/GTX637675/GTX637675_T-44837_20221111_WB_C_D_22111518_626.webp "Anti-beta Actin antibody [HL1926] used in Western Blot (WB). GTX637675")
![Anti-beta Actin antibody [HL1926] used in IHC (Paraffin sections) (IHC-P). GTX637675](/upload/website/prouct_img/normal/GTX637675/GTX637675_T-44837_20221111_IHC-P_M_22111518_644.webp "Anti-beta Actin antibody [HL1926] used in IHC (Paraffin sections) (IHC-P). GTX637675")
![Anti-beta Actin antibody [HL1926] used in Western Blot (WB). GTX637675](/upload/website/prouct_img/normal/GTX637675/GTX637675_T-44837_20221111_WB_Z_22111518_555.webp "Anti-beta Actin antibody [HL1926] used in Western Blot (WB). GTX637675")
![Anti-beta Actin antibody [HL1926] used in Western Blot (WB). GTX637675](/upload/website/prouct_img/normal/GTX637675/GTX637675_45180_20231110_WB_multiple_species_23112021_692.webp "Anti-beta Actin antibody [HL1926] used in Western Blot (WB). GTX637675")
![Anti-beta Actin antibody [HL1926] used in Western Blot (WB). GTX637675](/upload/website/prouct_img/normal/GTX637675/GTX637675_45257_20231215_WB_Sensitivity_24012217_356.webp "Anti-beta Actin antibody [HL1926] used in Western Blot (WB). GTX637675")
![Anti-beta Actin antibody [HL1926] used in Western Blot (WB). GTX637675](/upload/website/prouct_img/normal/GTX637675/GTX637675_T-44837_20221104_WB_M_R_22110919_481.webp "Anti-beta Actin antibody [HL1926] used in Western Blot (WB). GTX637675")
![Anti-beta Actin antibody [HL1926] used in Western Blot (WB). GTX637675](/upload/website/support/rewardsprogram/normal/genetex_GTX637675_WB_1_20231221_23122022_996.webp "Anti-beta Actin antibody [HL1926] used in Western Blot (WB). GTX637675")
