beta Actin抗体

Name: beta Actin抗体
Brand: GeneTex
SKU: GTX100313
Price: 4000.00 RMB

Anti-beta Actin antibody used in Western Blot (WB). GTX100313

Anti-beta Actin antibody used in IHC (Paraffin sections) (IHC-P). GTX100313

Anti-beta Actin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100313

目录号 GTX100313

文献引用 8

宿主	Rabbit
克隆	Polyclonal
同种型	IgG
实验应用	WB, ICC/IF, IHC-P
种属反应	Human, Mouse, Drosophila, Cat, Dog
产品包装	100 μl (¥4000), 25 μl (¥1700)

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View product citations for antibody GTX100313 on CiteAb

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认

实验应用	稀释倍数
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000

WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

42 kDa. ( Note )

阳性对照

NIH-3T3 , Raji , 293T

预测种属反应

Rat, Rabbit, Sheep, Bovine, Chicken, Guinea pig, Xenopus tropicalis, Chimpanzee, Rhesus Monkey(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1.16 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human beta Actin. The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_11174236

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

别名

actin beta , BRWS1 , PS1TP5BP1

细胞定位

Cytoplasm , cytoskeleton

功能与背景

This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. [provided by RefSeq]

数据库

Gene ID: 60 ACTB
UniProt: P60709 ACTB

研究领域

產品图片

GTX100313 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with beta Actin antibody (GTX100313) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100313 IHC-P Image

beta Actin antibody detects beta Actin protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded cat pancreas.
beta Actin stained by beta Actin antibody (GTX100313) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX100313 IHC-P Image

beta Actin antibody detects beta Actin protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
beta Actin stained by beta Actin antibody (GTX100313) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX100313 WB Image

Sample (30 ug of whole cell lysate)
A: Raji
10% SDS PAGE
Beta-actin antibody
GTX100313 diluted at 1:1000

GTX100313 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Actin antibody (GTX100313) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100313 WB Image

Sample (30 ug of whole cell lysate)
A: NIH-3T3
10% SDS PAGE
GTX100313 diluted at 1:5000

GTX100313 IHC-P Image

beta Actin antibody detects beta Actin protein at cytosol on mouse prostate by immunohistochemical analysis.
Sample: Paraffin-embedded mouse prostate.
beta Actin antibody (GTX100313) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX100313 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (10 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Actin antibody (GTX100313) diluted at 1:5000.

GTX100313 ICC/IF Image

beta Actin antibody detects beta Actin protein at cytoskeleton by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: beta Actin stained by beta Actin antibody (GTX100313) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar= 10 μm.

GTX100313 IHC-P Image

Immunohistochemical analysis of paraffin-embedded U373 xenograft, using beta Actin(GTX100313) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX100313 WB Image

Various whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with beta Actin antibody (GTX100313) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100313 WB Image

GTX100313 IHC-P Image

beta Actin antibody detects beta Actin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded dog muscle.
beta Actin stained by beta Actin antibody (GTX100313) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX100313 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with beta Actin antibody (GTX100313) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Drosophila Act5C is the homolog of mammalian beta Actin. (Gene ID: 31521/ UniProt: P10987)

文献引用

Application Reference

Su MT et al. Pharmaceuticals (Basel) 2022; 15 (12) Aspirin Inhibits Fibronectin Expression and Reverses Fibronectin-Mediated Cell Invasiveness by Activating Akt Signaling in Preeclampsia.

Lin JW et al. Cell Death Discov 2021; 7 (1) : 35 Dexamethasone accelerates muscle regeneration by modulating kinesin-1-mediated focal adhesion signals.

实验应用 : WB

种属反应 : Mouse

Christen KE et al. Int J Biochem Cell Biol 2019; 112 : 24-38 Psammaplysin F increases the efficacy of bortezomib and sorafenib through regulation of stress granule formation.

实验应用 : WB

种属反应 : Human

Li PH et al. Eur J Med Chem 2018; 145 : 498-510 Synthesis of carbazole derivatives containing chalcone analogs as non-intercalative topoisomerase II catalytic inhibitors and apoptosis inducers.

实验应用 : WB

种属反应 : Human

Andreev J et al. Mol Cancer Ther 2017; (Epub) Bispecific Antibodies and Antibody-Drug Conjugates (ADCs) Bridging HER2 and Prolactin Receptor Improve Efficacy of HER2 ADCs.

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Application Reference

Su MT et al. Pharmaceuticals (Basel) 2022; 15 (12) Aspirin Inhibits Fibronectin Expression and Reverses Fibronectin-Mediated Cell Invasiveness by Activating Akt Signaling in Preeclampsia.

Lin JW et al. Cell Death Discov 2021; 7 (1):35 Dexamethasone accelerates muscle regeneration by modulating kinesin-1-mediated focal adhesion signals.

实验应用 : WB

种属反应 : Mouse

Christen KE et al. Int J Biochem Cell Biol 2019; 112:24-38 Psammaplysin F increases the efficacy of bortezomib and sorafenib through regulation of stress granule formation.

实验应用 : WB

种属反应 : Human

Li PH et al. Eur J Med Chem 2018; 145:498-510 Synthesis of carbazole derivatives containing chalcone analogs as non-intercalative topoisomerase II catalytic inhibitors and apoptosis inducers.

实验应用 : WB

种属反应 : Human

Andreev J et al. Mol Cancer Ther 2017; (Epub) Bispecific Antibodies and Antibody-Drug Conjugates (ADCs) Bridging HER2 and Prolactin Receptor Improve Efficacy of HER2 ADCs.

Claude S et al. Mol Nutr Food Res 2014; 58 (5):1016-27 Flavanol metabolites reduce monocyte adhesion to endothelial cells through modulation of expression of genes via p38-MAPK and p65-Nf-kB pathways.

实验应用 : WB

种属反应 : Human

Sinanoglu O et al. Urology 2012; 80 (6):1392.e1-6 The protective effects of spirulina in * induced nephrotoxicity and urotoxicity in rats.

实验应用 : WB

种属反应 : Mouse

Tseng HL et al. Toxicol Appl Pharmacol 2012; 264 (1):104-13 Quercetin 3-O-methyl ether protects FL83B cells from copper induced oxidative stress through the PI3K/Akt and MAPK/Erk pathway.

实验应用 : WB

种属反应 : Mouse

SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf

产品包装	报价 (¥)
100 μl	¥ 4000
25 μl	¥ 1700
Qty

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	http://www.neobioscience.com
	genetex@neobioscience.com

beta Actin抗体

目录号 GTX100313

宿主

克隆

同种型

实验应用

种属反应

产品应用

应用说明

预期分子量

阳性对照

预测种属反应

产品属性

形式

存储溶液

存放说明

浓度

免疫原种属

免疫原

纯化方式

偶联

RRID

注意事项

相关讯息

别名

细胞定位

功能与背景

数据库

研究领域

產品图片

GTX100313 WB Image

GTX100313 IHC-P Image

GTX100313 IHC-P Image

GTX100313 WB Image

GTX100313 WB Image

GTX100313 WB Image

GTX100313 IHC-P Image

GTX100313 WB Image

GTX100313 ICC/IF Image

GTX100313 IHC-P Image

GTX100313 WB Image

GTX100313 WB Image

GTX100313 IHC-P Image

GTX100313 WB Image

文献引用

Application Reference

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