产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:5000-1:20000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
| Assay dependent |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
阳性对照
rat liver , Neuro2A , CD830 , NIH-3T3 , Raw264.7 , C2C12 , 293T , A431 , HeLa , HepG2
预测种属反应
Rabbit, Sheep, Bovine, Dog, Chicken, Guinea pig, Xenopus tropicalis, Chimpanzee, Rhesus Monkey(>80% identity)
产品属性
形式
Liquid
存储溶液
PBS, 1% BSA, 20% Glycerol
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
0.26 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant protein encompassing a sequence within the center region of human beta Actin. The exact sequence is proprietary.
纯化方式
Purified by antigen-affinity chromatography.
偶联
Unconjugated
RRID
AB_10618080
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
actin beta , BRWS1 , PS1TP5BP1
细胞定位
Cytoplasm , cytoskeleton
功能与背景
This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. [provided by RefSeq]
数据库
研究领域
產品图片
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GTX110564 WB Image
beta Actin antibody detects beta Actin protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Actin antibody (GTX110564) diluted at a dilution of 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX110564 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (10 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Actin antibody (GTX110564) diluted at 1:15000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX110564 WB Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with beta Actin antibody (GTX110564) diluted at 1:10000 and competitor's antibody (sc-130656) diluted at 1:100. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.
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GTX110564 WB Image
beta Actin antibody detects ACTB protein by western blot analysis.
A. 30 μg drosophila lysate/extract
10% SDS-PAGE
beta Actin antibody (GTX110564) dilution: 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX110564 IHC-P Image
beta Actin antibody detects beta Actin protein at cell membrane and cytoplasm in mouse pancreas by immunohistochemical analysis.
Sample: Paraffin-embedded mouse pancreas.
beta Actin antibody (GTX110564) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX110564 WB Image
beta Actin antibody detects beta Actin protein by western blot analysis. Rat tissue extracts (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with beta Actin antibody (GTX110564) diluted by 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX110564 ICC/IF Image
beta Actin antibody detects beta Actin protein at cytoskeleton by immunofluorescent analysis.
Sample: HeLa cells were fixed in 0.5% Triton X-100 for 1 min, then ice-cold methanol for 5 min.
Green: beta Actin protein stained by beta Actin antibody (GTX110564) diluted at 1:500.
Blue: Hoechst 33342 staining.
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GTX110564 WB Image
Various whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with beta Actin antibody (GTX110564) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX110564 WB Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Actin antibody (GTX110564) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX110564 IHC-P Image
beta Actin antibody detects beta Actin protein at cell membrane and cytoplasm in rat liver by immunohistochemical analysis.
Sample: Paraffin-embedded rat liver.
beta Actin antibody (GTX110564) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX110564 IP Image
Immunoprecipitation of beta Actin protein from 293T whole cell extracts using 5 μg of beta Actin antibody (GTX110564).
Western blot analysis was performed using beta Actin antibody (GTX110564).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
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GTX110564 WB Image
Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Actin antibody (GTX110564) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX110564 WB Image
Yeast lysate (10 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with beta Actin antibody (GTX110564) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX110564 IHC-P Image
beta Actin antibody detects beta Actin protein at cytoplasm in mouse cervix by immunohistochemical analysis.
Sample: Paraffin-embedded mouse cervix.
beta Actin antibody (GTX110564) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX110564 WB Image
The data was published in the journal Front Physiol in 2017.PMID: 28588500
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GTX110564 WB Image
The data was published in the journal PLoS One in 2016.PMID: 27486856
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GTX110564 WB Image
The data was published in the journal Redox Biol in 2014. PMID: 24936442
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GTX110564 WB Image
The data was published in the journal PLoS Pathog in 2019. PMID: 30818369
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GTX110564 WB Image
The data was published in the journal PLoS Pathog in 2019. PMID: 30818369
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GTX110564 WB Image
The data was published in the journal Aging Cell in 2017. PMID: 27623715
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GTX110564 WB Image
The data was published in the journal Aging Cell in 2017. PMID: 27623715
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GTX110564 WB Image
The data was published in the journal Aging Cell in 2017. PMID: 27623715
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文献引用
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