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beta Actin抗体

Anti-beta Actin antibody used in Western Blot (WB). GTX21801
Anti-beta Actin antibody used in Western Blot (WB). GTX21801
Anti-beta Actin antibody used in IHC (Paraffin sections) (IHC-P). GTX21801

目录号 GTX21801

宿主

Rabbit

克隆

Polyclonal

同种型

IgG

实验应用

WB, ICC/IF, IHC-P, ELISA

种属反应

Human, Mouse, Frog
产品包装
100 μg (¥5000)

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:1000-1:4000
ICC/IF 1:500-1:2000
IHC-P Assay dependent
ELISA 1:10000-1:40000
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

42 kDa. ( Note )

产品属性

形式

Liquid

存储溶液

20mM Potassium Phosphate, 150mM NaCl

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Synthetic peptide corresponding to C-Terminal region near amino acids 350-375 of Human beta Actin.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_1240407

注意事项

仅供实验室使用。不适用于人类或动物的任何临床,治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

actin beta , BRWS1 , PS1TP5BP1

细胞定位

Cytoplasm, cytoskeleton,Nucleus

功能与背景

The two major cytoskeletal proteins implicated in cell motility are actin and myosin. Actin and myosin are constituents of many cell types and are involved in a myriad of cellular processes including locomotion, secretion, cytoplasmic streaming, phagocytosis and cytokinesis. Although actin is one of the most conserved eukaryotic proteins, it is expressed in mammals and birds as at least six isoforms characterized by electrophoresis and amino acid sequence analysis. Four of them represent the differentiation markers of muscle tissues and two are found in practically all cells.

数据库

研究领域

產品图片

Anti-beta Actin antibody used in Western Blot (WB). GTX21801

GTX21801 WB Image

Western Blot of Rabbit Anti-Beta Actin Antibody (GTX21801). Lane 1: molecular weight. Lane 2: human embryonic kidney 293. Lane 3: human lung carcinoma A549. Lane 4: mouse brain. Load: 35 μg per lane. Primary antibody: Beta Actin antibody at 1:1,500 for overnight at 4ºC. Secondary antibody: infrared rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4ºC. Predicted/Observed size: ~42 kDa corresponding to beta Actin (arrowhead). Other band(s): none

Anti-beta Actin antibody used in Western Blot (WB). GTX21801

GTX21801 WB Image

WB analysis of various samples using GTX21801 beta Actin antibody.
Lane 1 : Protein ladder
Lane 2 : 293T whole cell lysate
Lane 3 : A549 whole cell lysate
Lane 4 : Mouse brain tissue lysate
Loading : 35 μg
Dilution : 1:500

Anti-beta Actin antibody used in IHC (Paraffin sections) (IHC-P). GTX21801

GTX21801 IHC-P Image

IHC-P analysis of rana pipiens tissue using GTX21801 beta Actin antibody.
Green : Primary antibody
Dilution : 1:200

文献引用

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SDS
Sodium Azide.pdf
产品包装 报价 (¥)
¥ 5000
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