产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
实验应用 |
稀释倍数 |
1 - 2 μg/mL |
2 μg/mL |
Assay dependent |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
阳性对照
HeLa Cell Slide(GTX25529) , HeLa Cell Lysate
产品属性
形式
Liquid
存储溶液
PBS
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Beta-actin antibody was raised against a 16 amino acid synthetic peptide from near the amino terminus of human beta-actin.The immunogen is located within amino acids 90 - 140 of Beta-actin.
纯化方式
Purified by antigen-affinity chromatography
偶联
Unconjugated
注意事项
仅供实验室使用。不适用于人类或动物的任何临床,治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
actin beta , BRWS1 , PS1TP5BP1
细胞定位
Cytoplasm, cytoskeleton,Nucleus
功能与背景
Actins are highly conserved proteins that are involved in cell motility, structure and integrity, processes that are crucial for tissue development and the development of organism. The actin cytoskeleton is one of the principal drivers of cell motility and is capable of responding to complex signaling cascades. Recent evidence suggests that it may play key roles in regulating apoptosis and aging. Beta actin is one of six different actin isoforms which have been identified. Like GAPDH, b-actin is constitutively expressed at high levels in almost all tissues and cell lines making it ideal for use as a loading control marker in immunoblots.
数据库
研究领域
產品图片
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GTX85117 WB Image
WB analysis of HeLa cell lysate using GTX85117 beta Actin antibody. Working concentration : (A) 1 and (B) 2 μg/ml
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GTX85117 ICC/IF Image
ICC/IF analysis of HeLa cells using GTX85117 beta Actin antibody. Working concentration : 2 μg/ml
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