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beta Amyloid (1-42)抗体 – Conformation Specific抗体 [GT622]

Orthogonal
Anti-beta Amyloid (1-42) antibody – Conformation Specific antibody [GT622] used in ELISA (ELISA). GTX635160
Anti-beta Amyloid (1-42) antibody – Conformation Specific antibody [GT622] used in ELISA (ELISA). GTX635160
Anti-beta Amyloid (1-42) antibody – Conformation Specific antibody [GT622] used in Dot blot (Dot). GTX635160
Anti-beta Amyloid (1-42) antibody – Conformation Specific antibody [GT622] used in IHC (Paraffin sections) (IHC-P). GTX635160
Anti-beta Amyloid (1-42) antibody – Conformation Specific antibody [GT622] used in IHC (Paraffin sections) (IHC-P). GTX635160
Anti-beta Amyloid (1-42) antibody – Conformation Specific antibody [GT622] used in IHC (Paraffin sections) (IHC-P). GTX635160

目录号 GTX635160

文献引用 1

宿主

 Mouse

克隆

 Monoclonal

克隆编号

 GT622

同种型

 IgG2b

实验应用

 IHC-P, Dot, ELISA, Sandwich ELISA

种属反应

 Human, Mouse
产品包装
100 μl (¥4000),
25 μl (¥1700)
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产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
IHC-P 1:100-1:2000
Dot Assay dependent
ELISA Assay dependent
Sandwich ELISA Assay dependent

Note :

Sandwich ELISA
Capture : GTX635160, Detection: GTX134510
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

87 kDa. ( Note )

预测种属反应

Rat, Rabbit, Bovine, Dog, Chicken, Pig, Xenopus tropicalis, Chimpanzee(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

0.27 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

The immunogen used to generate this antibody corresponds to Beta amyloid (1-42).

纯化方式

Affinity purified by Protein A.

偶联

Unconjugated

RRID

AB_2888506

注意事项

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

相关讯息

别名

amyloid beta precursor protein , AAA , ABETA , ABPP , AD1 , APPI , CTFgamma , CVAP , PN-II , PN2 , preA4

细胞定位

Cell membrane,Membrane,Cytoplasmic vesicle,Cytoplasm

功能与背景

This gene encodes a cell surface receptor and transmembrane precursor protein that is cleaved by secretases to form a number of peptides. Some of these peptides are secreted and can bind to the acetyltransferase complex APBB1/TIP60 to promote transcriptional activation, while others form the protein basis of the amyloid plaques found in the brains of patients with Alzheimer disease. In addition, two of the peptides are antimicrobial peptides, having been shown to have bacteriocidal and antifungal activities. Mutations in this gene have been implicated in autosomal dominant Alzheimer disease and cerebroarterial amyloidosis (cerebral amyloid angiopathy). Multiple transcript variants encoding several different isoforms have been found for this gene. [provided by RefSeq, Aug 2014]

数据库

研究领域

產品图片

Anti-beta Amyloid (1-42) antibody – Conformation Specific antibody [GT622] used in ELISA (ELISA). GTX635160

GTX635160 ELISA Image

Sandwich ELISA detection of beta Amyloid (1-42)–peptide aggregate using GTX635160 as capture antibody at concentration of 10 μg/mL and GTX134510 as detection antibody at concentration of 0.45 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:2000 and used to detect the primary antibody.

Anti-beta Amyloid (1-42) antibody – Conformation Specific antibody [GT622] used in ELISA (ELISA). GTX635160

GTX635160 ELISA Image

Indirect ELISA analysis was performed by coating plate with 100 μL of beta Amyloid (1-42) peptide aggregate at concentration of 10 μg/mL. The coated protein is detected with beta Amyloid (1-42) antibody – Conformation Specific antibody [GT622] (GTX635160) at concentrations ranging from 3.06 to 2230 ng/mL. Mouse IgG antibody (HRP) (GTX213111-01) was diluted at 1:2000 and used to detect the primary antibody.

Anti-beta Amyloid (1-42) antibody – Conformation Specific antibody [GT622] used in Dot blot (Dot). GTX635160

GTX635160 Dot Image

Dot blot analysis of 100 ng beta Amyloid 1-42 oligomers or monomers, using beta Amyloid (1-42) antibody – Conformation Specific antibody [GT622] (GTX635160) at 1:10000, 1:20000, 1:40000, and 1:80000 dilution.

Anti-beta Amyloid (1-42) antibody – Conformation Specific antibody [GT622] used in IHC (Paraffin sections) (IHC-P). GTX635160

GTX635160 IHC-P Image

Orthogonal

beta Amyloid (1-42) antibody – Conformation Specific antibody [GT622] detects beta Amyloid protein aggregates by immunohistochemical analysis in the hippocampus of 3xTg-AD transgenic mouse brain (left), but not in age- and gender- matched normal mouse brain (right).
Sample: Paraffin-embedded 3xTg-AD transgenic mouse brain (left) and healthy mouse brain (right).
Green: beta Amyloid (1-42) antibody – Conformation Specific antibody [GT622] (GTX635160) diluted at 1:2000.
Red: NeuN stained by NeuN antibody (GTX132974) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
This image was provided courtesy of a customer review.

Anti-beta Amyloid (1-42) antibody – Conformation Specific antibody [GT622] used in IHC (Paraffin sections) (IHC-P). GTX635160

GTX635160 IHC-P Image

Orthogonal

beta Amyloid (1-42) antibody – Conformation Specific antibody [GT622] detects beta amyloid protein aggregates by immunohistochemical analysis in the occipital lobe of Alzheimer's disease brain (left), but not in normal human brain(right).
Sample: Paraffin-embedded Human Brain (Alzheimer's Disease + Normal) tissue slides (GTX49382).
beta Amyloid protein aggregates stained by beta Amyloid (1-42) antibody – Conformation Specific antibody [GT622] (GTX635160) diluted at 1:2000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-beta Amyloid (1-42) antibody – Conformation Specific antibody [GT622] used in IHC (Paraffin sections) (IHC-P). GTX635160

GTX635160 IHC-P Image

Orthogonal

beta Amyloid (1-42) antibody – Conformation Specific antibody [GT622] detects beta Amyloid protein aggregates by immunohistochemical analysis in the occipital lobe of Alzheimer's disease brain (left), but not in normal human brain(right).
Sample: Paraffin-embedded Human Brain (Alzheimer's Disease + Normal) tissue slides (GTX49382).
beta Amyloid protein aggregates stained by beta Amyloid (1-42) antibody – Conformation Specific antibody [GT622] (GTX635160) diluted at 1:2000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

文献引用

Application Reference

Bassil R et al. Nat Commun 2021; 12 (1) : 5220 Improved modeling of human AD with an automated culturing platform for iPSC neurons, astrocytes and microglia.
实验应用 : ELISA
种属反应 : Human
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产品包装 报价 (¥)
¥ 4000
¥ 1700
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