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beta Catenin抗体 [GT3171]

KO/KD Comparison
Anti-beta Catenin antibody [GT3171] used in Western Blot (WB). GTX632676
Anti-beta Catenin antibody [GT3171] used in Western Blot (WB). GTX632676
Anti-beta Catenin antibody [GT3171] used in IHC (Paraffin sections) (IHC-P). GTX632676
Anti-beta Catenin antibody [GT3171] used in Western Blot (WB). GTX632676
Anti-beta Catenin antibody [GT3171] used in Western Blot (WB). GTX632676
Anti-beta Catenin antibody [GT3171] used in IHC (Paraffin sections) (IHC-P). GTX632676
Anti-beta Catenin antibody [GT3171] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX632676
Anti-beta Catenin antibody [GT3171] used in IHC (Paraffin sections) (IHC-P). GTX632676
Anti-beta Catenin antibody [GT3171] used in IHC (Paraffin sections) (IHC-P). GTX632676
Anti-beta Catenin antibody [GT3171] used in Western Blot (WB). GTX632676
Anti-beta Catenin antibody [GT3171] used in Western Blot (WB). GTX632676

目录号 GTX632676

文献引用 1

宿主

 Mouse

克隆

 Monoclonal

克隆编号

 GT3171

同种型

 IgG1

实验应用

 WB, ICC/IF, IHC-P

种属反应

 Human, Mouse, Cat, Dog
产品包装
100 μl (¥4000),
25 μl (¥1700)
查看所有 beta Catenin 的产品

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

85 kDa. ( Note )

阳性对照

A549 , H1299 , HCT116 , HeLa

预测种属反应

Rat, Zebrafish, Sheep, Bovine, Chicken, Pig, Xenopus tropicalis, Rhesus Monkey(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1.18 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the N-terminus region of human beta Catenin. The exact sequence is proprietary.

纯化方式

Affinity purified by Protein G.

偶联

Unconjugated

RRID

AB_2888327

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

catenin beta 1 , CTNNB , EVR7 , MRD19 , armadillo

细胞定位

Cytoplasm , Nucleus , cytoskeleton

功能与背景

Beta-catenin is an adherens junction protein. Adherens junctions (AJs; also called the zonula adherens) are critical for the establishment and maintenance of epithelial layers, such as those lining organ surfaces. AJs mediate adhesion between cells, communicate a signal that neighboring cells are present, and anchor the actin cytoskeleton. In serving these roles, AJs regulate normal cell growth and behavior. At several stages of embryogenesis, wound healing, and tumor cell metastasis, cells form and leave epithelia. This process, which involves the disruption and reestablishment of epithelial cell-cell contacts, may be regulated by the disassembly and assembly of AJs. AJs may also function in the transmission of the 'contact inhibition' signal, which instructs cells to stop dividing once an epithelial sheet is complete.[supplied by OMIM]

数据库

研究领域

產品图片

Anti-beta Catenin antibody [GT3171] used in Western Blot (WB). GTX632676

GTX632676 WB Image

KO/KD

Wild-type (WT) and beta Catenin knockout (KO) 293T cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with beta Catenin antibody [GT3171] (GTX632676) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-beta Catenin antibody [GT3171] used in Western Blot (WB). GTX632676

GTX632676 WB Image

KO/KD

Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with beta Catenin antibody [GT3171] (GTX632676) diluted at 1:1000.

Anti-beta Catenin antibody [GT3171] used in IHC (Paraffin sections) (IHC-P). GTX632676

GTX632676 IHC-P Image

beta Catenin antibody [GT3171] detects beta Catenin protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
Red: beta Catenin stained by beta Catenin antibody [GT3171] (GTX632676) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-beta Catenin antibody [GT3171] used in Western Blot (WB). GTX632676

GTX632676 WB Image

Comparison

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with beta Catenin antibody (GTX632676) diluted at 1:500 and competitor's antibody (# Highly Cited Antibody ) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-beta Catenin antibody [GT3171] used in Western Blot (WB). GTX632676

GTX632676 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with beta Catenin antibody [GT3171] (GTX632676) diluted at 1:1000.

Anti-beta Catenin antibody [GT3171] used in IHC (Paraffin sections) (IHC-P). GTX632676

GTX632676 IHC-P Image

beta Catenin antibody [GT3171] detects beta Catenin protein at cell membrane and cytoplasm in mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
beta Catenin antibody [GT3171] (GTX632676) diluted at 1:400.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-beta Catenin antibody [GT3171] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX632676

GTX632676 ICC/IF Image

beta Catenin antibody detects beta Catenin protein at cell membrane and cytoplasm by immunofluorescent analysis.
Sample: MDCK cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: beta Catenin stained by beta Catenin antibody (GTX632676) diluted at 1:1000.

Anti-beta Catenin antibody [GT3171] used in IHC (Paraffin sections) (IHC-P). GTX632676

GTX632676 IHC-P Image

beta Catenin antibody detects beta Catenin protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded cat colon.
beta Catenin stained by beta Catenin antibody (GTX632676) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-beta Catenin antibody [GT3171] used in IHC (Paraffin sections) (IHC-P). GTX632676

GTX632676 IHC-P Image

beta Catenin antibody detects beta Catenin protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded cat liver.
beta Catenin stained by beta Catenin antibody (GTX632676) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-beta Catenin antibody [GT3171] used in Western Blot (WB). GTX632676

GTX632676 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with beta Catenin antibody (GTX632676) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-beta Catenin antibody [GT3171] used in Western Blot (WB). GTX632676

GTX632676 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with beta Catenin antibody (GTX632676) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

文献引用

Application Reference

Chih-Hsin Hsu et al. Int J Mol Sci 2021; 22 (19) miR-29a-3p/THBS2 Axis Regulates PAH-Induced Cardiac Fibrosis.
实验应用 : WB
种属反应 : Human, Mouse
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产品包装 报价 (¥)
¥ 4000
¥ 1700
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