beta Tubulin 3/ Tuj1抗体 [GT11710]

Name: beta Tubulin 3/ Tuj1抗体 [GT11710]
Brand: GeneTex
SKU: GTX631836
Price: 4000.00 RMB
Rating: 5 (2 reviews)

Anti-beta Tubulin 3/ Tuj1 antibody [GT11710] used in IHC (Frozen sections) (IHC-Fr). GTX631836

Anti-beta Tubulin 3/ Tuj1 antibody [GT11710] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX631836

Anti-beta Tubulin 3/ Tuj1 antibody [GT11710] used in Western Blot (WB). GTX631836

Anti-beta Tubulin 3/ Tuj1 antibody [GT11710] used in Immunoprecipitation (IP). GTX631836

Anti-beta Tubulin 3/ Tuj1 antibody [GT11710] used in IHC (Paraffin sections) (IHC-P). GTX631836

目录号 GTX631836

文献引用 33

宿主	Mouse
克隆	Monoclonal
克隆编号	GT11710
同种型	IgG2b
实验应用	WB, ICC/IF, IHC-P, IHC-Fr, IP
种属反应	Human, Mouse, Rat, Fish
产品包装	100 μl (¥4000), 25 μl (¥1700)

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View product citations for antibody GTX631836 on CiteAb

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认

实验应用	稀释倍数
WB	1:500-1:20000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000
IHC-Fr	1:100-1:1000
IP	1:100-1:500

WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

50 kDa. ( Note )

阳性对照

HeLa , SK-N-SH , IMR32 , SK-N-AS , mouse brain , rat brain

产品说明

beta III Tubulin antibody [GT11710] specifically recognizes endogenous levels of beta III Tubulin based on the immunogen sequence.

预测种属反应

Bovine, Chicken, Chimpanzee(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human beta Tubulin 3/ Tuj1. The exact sequence is proprietary.

纯化方式

Affinity purified by Protein A.

偶联

Unconjugated

RRID

AB_2814952

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

别名

tubulin beta 3 class III , CDCBM , CDCBM1 , CFEOM3 , CFEOM3A , FEOM3 , TUBB4 , beta-4

功能与背景

This gene encodes a class III member of the beta tubulin protein family. Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. This protein is primarily expressed in neurons and may be involved in neurogenesis and axon guidance and maintenance. Mutations in this gene are the cause of congenital fibrosis of the extraocular muscles type 3. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 6. [provided by RefSeq]

数据库

Gene ID: 10381 TUBB3
UniProt: Q13509 TUBB3

研究领域

產品图片

GTX631836 IHC-Fr Image

beta Tubulin 3/ TUJ1 antibody [GT11710] detects beta Tubulin 3/ TUJ1 protein by immunohistochemical analysis.
Sample: Frozen sectioned adult mouse retina.
Red: beta Tubulin 3/ TUJ1 protein stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

GTX631836 ICC/IF Image

beta Tubulin 3/ TUJ1 antibody [GT11710] detects beta Tubulin 3/ TUJ1 protein expression by immunofluorescent analysis.
Sample: Cultured rat E18 primary hippocampal neuron. Cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: beta Tubulin 3/ TUJ1 protein stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

GTX631836 IHC-Fr Image

beta Tubulin 3/ TUJ1 antibody [GT11710] detects beta Tubulin 3/ TUJ1 protein by immunohistochemical analysis.
Sample: Frozen sectioned E13.5 rat brain.
Red: beta Tubulin 3/ TUJ1 protein stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX131836) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

GTX631836 IHC-Fr Image

beta III Tubulin antibody [GT11710] detects beta III Tubulin proteins on embryonic mouse brain by immunohistochemical analysis.
Sample:Frozen section of embryonic mouse brain (mE18.5).
Red: beta III Tubulin antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: DAPI

GTX631836 IHC-Fr Image

beta Tubulin 3/ TUJ1 antibody [GT11710] detects beta Tubulin 3/ TUJ1 protein by immunohistochemical analysis.
Sample: Frozen sectioned E13.5 rat brain.
Green: SOX2 protein stained by SOX2 antibody [N1C3] (GTX101507) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1 protein stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX131836) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

GTX631836 WB Image

Mouse tissue extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

GTX631836 IP Image

Immunoprecipitation of beta III Tubulin protein from SK-N-SH whole cell extracts using 5 μg of beta III Tubulin antibody [GT11710] (GTX631836).
Western blot analysis was performed using beta III Tubulin antibody [GT11710] (GTX631836).
EasyBlot anti-Mouse IgG (GTX221667-01) was used as a secondary reagent.

GTX631836 WB Image

beta Tubulin 3/ TUJ1 antibody detects beta Tubulin 3/ TUJ1 protein by western blot analysis. Various whole cell extracts (20 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Tubulin 3/ TUJ1 antibody (GTX631836) diluted at a dilution of 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

GTX631836 WB Image

Wild-type (WT) and beta Tubulin 3/ Tuj1 knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:2500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

GTX631836 IHC-P Image

beta Tubulin 3/ TUJ1 antibody [GT11710] detects beta Tubulin 3/ TUJ1 protein at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX631836 WB Image

Various tissue extracts (10 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:20000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.