产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:500-1:3000 |
| Assay dependent |
| Assay dependent |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
产品属性
形式
Liquid
存储溶液
PBS
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Synthetic peptide of human beta Tubulin 3/ Tuj1
纯化方式
Affinity purified by Protein A.
偶联
Unconjugated
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
tubulin beta 3 class III , CDCBM , CDCBM1 , CFEOM3 , CFEOM3A , FEOM3 , TUBB4 , beta-4
细胞定位
Cytoplasm, cytoskeleton
功能与背景
This gene encodes a class III member of the beta tubulin protein family. Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. This protein is primarily expressed in neurons and may be involved in neurogenesis and axon guidance and maintenance. Mutations in this gene are the cause of congenital fibrosis of the extraocular muscles type 3. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 6. [provided by RefSeq, Oct 2010]
数据库
研究领域
產品图片
![Anti-beta Tubulin 3/ Tuj1 antibody [HL1709] used in Western Blot (WB). GTX637308](/templates/default/js/lightbox/images/loading.gif "Anti-beta Tubulin 3/ Tuj1 antibody [HL1709] used in Western Blot (WB). GTX637308")
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GTX637308 WB Image
Wild-type (WT) and beta Tubulin 3/ Tuj1 knockout (KO) HeLa cell extracts (10 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Tubulin 3/ Tuj1 antibody [HL1709] (GTX637308) diluted at 1:4000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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GTX637308 WB Image
Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Tubulin 3/ Tuj1 antibody [HL1709] (GTX637308) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX637308 WB Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Tubulin 3/ Tuj1 antibody [HL1709] (GTX637308) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
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GTX637308 IHC-P Image
beta Tubulin 3/ Tuj1 antibody [HL1709] detects beta Tubulin 3/ Tuj1 protein by immunohistochemical analysis.Sample: Paraffin-embedded rat cerebellum.Green: beta Tubulin 3/ Tuj1 stained by beta Tubulin 3/ Tuj1 antibody [HL1709] (GTX637308) diluted at 1:100.Blue: Fluoroshield with DAPI (GTX30920).Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX637308 IHC-P Image
beta Tubulin 3/ Tuj1 antibody [HL1709] detects beta Tubulin 3/ Tuj1 protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse cerebellum.Green: beta Tubulin 3/ Tuj1 stained by beta Tubulin 3/ Tuj1 antibody [HL1709] (GTX637308) diluted at 1:100.Blue: Fluoroshield with DAPI (GTX30920).Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX637308 ICC/IF Image
beta Tubulin 3/ Tuj1 antibody [HL1709] detects beta Tubulin 3/ Tuj1 protein at cytoskeleton by immunofluorescent analysis.Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: beta Tubulin 3/ Tuj1 stained by beta Tubulin 3/ Tuj1 antibody [HL1709] (GTX637308) diluted at 1:250.Blue: Fluoroshield with DAPI (GTX30920).
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GTX637308 WB Image
Drosophila tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with beta Tubulin 3/ Tuj1 antibody [HL1709] (GTX637308) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.
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GTX637308 ICC/IF Image
beta Tubulin 3/ Tuj1 antibody [HL1709] detects beta Tubulin 3/ Tuj1 protein by immunofluorescent analysis.Sample: SH-SY-5Y cells were fixed in ice-cold MeOH for 10 min.Green: beta Tubulin 3/ Tuj1 stained by beta Tubulin 3/ Tuj1 antibody [HL1709] (GTX637308) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.Blue: Fluoroshield with DAPI (GTX30920).
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文献引用
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产品评论
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![Anti-beta Tubulin 3/ Tuj1 antibody [HL1709] used in Western Blot (WB). GTX637308](/upload/website/prouct_img/small/GTX637308/GTX637308_T-44774_20221125_WB_KO_watermark_22112723_537.webp "Anti-beta Tubulin 3/ Tuj1 antibody [HL1709] used in Western Blot (WB). GTX637308")
![Anti-beta Tubulin 3/ Tuj1 antibody [HL1709] used in Western Blot (WB). GTX637308](/upload/website/prouct_img/small/GTX637308/GTX637308_T-44774_20221111_WB_M_R_22111518_185.webp "Anti-beta Tubulin 3/ Tuj1 antibody [HL1709] used in Western Blot (WB). GTX637308")
![Anti-beta Tubulin 3/ Tuj1 antibody [HL1709] used in Western Blot (WB). GTX637308](/upload/website/prouct_img/small/GTX637308/GTX637308_44914_20230106_WB_TPM_watermark_23010922_324.webp "Anti-beta Tubulin 3/ Tuj1 antibody [HL1709] used in Western Blot (WB). GTX637308")
![Anti-beta Tubulin 3/ Tuj1 antibody [HL1709] used in IHC (Paraffin sections) (IHC-P). GTX637308](/upload/website/prouct_img/small/GTX637308/GTX637308_44914_20230217_IHC-P_R_23021401_499.webp "Anti-beta Tubulin 3/ Tuj1 antibody [HL1709] used in IHC (Paraffin sections) (IHC-P). GTX637308")
![Anti-beta Tubulin 3/ Tuj1 antibody [HL1709] used in IHC (Paraffin sections) (IHC-P). GTX637308](/upload/website/prouct_img/small/GTX637308/GTX637308_44914_20230217_IHC-P_M_23021401_223.webp "Anti-beta Tubulin 3/ Tuj1 antibody [HL1709] used in IHC (Paraffin sections) (IHC-P). GTX637308")
![Anti-beta Tubulin 3/ Tuj1 antibody [HL1709] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637308](/upload/website/prouct_img/small/GTX637308/GTX637308_T-44774_20221209_ICC_IF_R_22122018_965.webp "Anti-beta Tubulin 3/ Tuj1 antibody [HL1709] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637308")
![Anti-beta Tubulin 3/ Tuj1 antibody [HL1709] used in Western Blot (WB). GTX637308](/upload/website/prouct_img/small/GTX637308/GTX637308_44914_20230324_WB_Drosophila_brain_23032819_173.webp "Anti-beta Tubulin 3/ Tuj1 antibody [HL1709] used in Western Blot (WB). GTX637308")
![Anti-beta Tubulin 3/ Tuj1 antibody [HL1709] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637308](/upload/website/prouct_img/small/GTX637308/GTX637308_T-44774_20221230_ICC_IF_22122901_860.webp "Anti-beta Tubulin 3/ Tuj1 antibody [HL1709] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637308")
![Anti-beta Tubulin 3/ Tuj1 antibody [HL1709] used in Western Blot (WB). GTX637308](/upload/website/prouct_img/normal/GTX637308/GTX637308_T-44774_20221125_WB_KO_watermark_22112723_537.webp "Anti-beta Tubulin 3/ Tuj1 antibody [HL1709] used in Western Blot (WB). GTX637308")
![Anti-beta Tubulin 3/ Tuj1 antibody [HL1709] used in Western Blot (WB). GTX637308](/upload/website/prouct_img/normal/GTX637308/GTX637308_T-44774_20221111_WB_M_R_22111518_185.webp "Anti-beta Tubulin 3/ Tuj1 antibody [HL1709] used in Western Blot (WB). GTX637308")
![Anti-beta Tubulin 3/ Tuj1 antibody [HL1709] used in Western Blot (WB). GTX637308](/upload/website/prouct_img/normal/GTX637308/GTX637308_44914_20230106_WB_TPM_watermark_23010922_324.webp "Anti-beta Tubulin 3/ Tuj1 antibody [HL1709] used in Western Blot (WB). GTX637308")
![Anti-beta Tubulin 3/ Tuj1 antibody [HL1709] used in IHC (Paraffin sections) (IHC-P). GTX637308](/upload/website/prouct_img/normal/GTX637308/GTX637308_44914_20230217_IHC-P_R_23021401_499.webp "Anti-beta Tubulin 3/ Tuj1 antibody [HL1709] used in IHC (Paraffin sections) (IHC-P). GTX637308")
![Anti-beta Tubulin 3/ Tuj1 antibody [HL1709] used in IHC (Paraffin sections) (IHC-P). GTX637308](/upload/website/prouct_img/normal/GTX637308/GTX637308_44914_20230217_IHC-P_M_23021401_223.webp "Anti-beta Tubulin 3/ Tuj1 antibody [HL1709] used in IHC (Paraffin sections) (IHC-P). GTX637308")
![Anti-beta Tubulin 3/ Tuj1 antibody [HL1709] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637308](/upload/website/prouct_img/normal/GTX637308/GTX637308_T-44774_20221209_ICC_IF_R_22122018_965.webp "Anti-beta Tubulin 3/ Tuj1 antibody [HL1709] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637308")
![Anti-beta Tubulin 3/ Tuj1 antibody [HL1709] used in Western Blot (WB). GTX637308](/upload/website/prouct_img/normal/GTX637308/GTX637308_44914_20230324_WB_Drosophila_brain_23032819_173.webp "Anti-beta Tubulin 3/ Tuj1 antibody [HL1709] used in Western Blot (WB). GTX637308")
![Anti-beta Tubulin 3/ Tuj1 antibody [HL1709] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637308](/upload/website/prouct_img/normal/GTX637308/GTX637308_T-44774_20221230_ICC_IF_22122901_860.webp "Anti-beta Tubulin 3/ Tuj1 antibody [HL1709] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637308")
