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beta Tubulin抗体

Anti-beta Tubulin antibody used in IHC (Paraffin sections) (IHC-P). GTX101279
Anti-beta Tubulin antibody used in IHC (Paraffin sections) (IHC-P). GTX101279
Anti-beta Tubulin antibody used in Western Blot (WB). GTX101279
Anti-beta Tubulin antibody used in Western Blot (WB). GTX101279
Anti-beta Tubulin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101279
Anti-beta Tubulin antibody used in IHC (Paraffin sections) (IHC-P). GTX101279
Anti-beta Tubulin antibody used in Western Blot (WB). GTX101279
Anti-beta Tubulin antibody used in IHC (Paraffin sections) (IHC-P). GTX101279
Anti-beta Tubulin antibody used in Western Blot (WB). GTX101279
Anti-beta Tubulin antibody used in IHC (Paraffin sections) (IHC-P). GTX101279
Anti-beta Tubulin antibody used in IHC (Paraffin sections) (IHC-P). GTX101279
Anti-beta Tubulin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101279

目录号 GTX101279

宿主

Rabbit

克隆

Polyclonal

同种型

IgG

实验应用

WB, ICC/IF, IHC-P

种属反应

Human, Mouse, Rat, Hamster
产品包装
100 μl (¥3590),
25 μl (¥1390)

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

阳性对照

293T , A431 , HeLa , HepG2 , A549 , H1299 , HCT116 , NIH-3T3 , *MCF7 , *Huh7

预测种属反应

Bovine, Chicken, Pig, Xenopus laevis, Chimpanzee, Rhesus Monkey(>80% identity)

产品属性

形式

Liquid

存储溶液

1XPBS (pH7), 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

2.44 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the center region of human beta Tubulin. The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_1952434

注意事项

仅供实验室使用。不适用于人类或动物的任何临床,治疗或诊断用途。不适合动物或人类食用。

相关讯息

產品图片

Anti-beta Tubulin antibody used in IHC (Paraffin sections) (IHC-P). GTX101279

GTX101279 IHC-P Image

beta Tubulin antibody detects beta Tubulin protein at cytoplasm in rat spinal cord by immunohistochemical analysis.
Sample: Paraffin-embedded rat spinal cord.
beta Tubulin antibody (GTX101279) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-beta Tubulin antibody used in IHC (Paraffin sections) (IHC-P). GTX101279

GTX101279 IHC-P Image

beta Tubulin antibody detects beta Tubulin protein at cytoplasm in rat spleen by immunohistochemical analysis.
Sample: Paraffin-embedded rat spleen.
beta Tubulin antibody (GTX101279) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-beta Tubulin antibody used in Western Blot (WB). GTX101279

GTX101279 WB Image

beta Tubulin antibody detects beta Tubulin protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Tubulin antibody (GTX101279) diluted at a dilution of 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-beta Tubulin antibody used in Western Blot (WB). GTX101279

GTX101279 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Tubulin antibody (GTX101279) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-beta Tubulin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101279

GTX101279 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using beta Tubulin(GTX101279) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

Anti-beta Tubulin antibody used in IHC (Paraffin sections) (IHC-P). GTX101279

GTX101279 IHC-P Image

beta Tubulin antibody detects beta Tubulin protein at cytoplasm on human breast cancer by immunohistochemical analysis.
Sample: Paraffin-embedded breast cancer.
beta Tubulin antibody (GTX101279) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-beta Tubulin antibody used in Western Blot (WB). GTX101279

GTX101279 WB Image

Sample (30 μg of whole cell lysate)
A: NIH-3T3
10% SDS PAGE
GTX101279 diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-beta Tubulin antibody used in IHC (Paraffin sections) (IHC-P). GTX101279

GTX101279 IHC-P Image

Immunohistochemical analysis of paraffin-embedded AGS xenograft, using beta Tubulin(GTX101279) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-beta Tubulin antibody used in Western Blot (WB). GTX101279

GTX101279 WB Image

Various whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with beta Tubulin antibody (GTX101279) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-beta Tubulin antibody used in IHC (Paraffin sections) (IHC-P). GTX101279

GTX101279 IHC-P Image

beta Tubulin antibody detects beta Tubulin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse spinal cord.
beta Tubulin stained by beta Tubulin antibody (GTX101279) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-beta Tubulin antibody used in IHC (Paraffin sections) (IHC-P). GTX101279

GTX101279 IHC-P Image

beta Tubulin antibody detects beta Tubulin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
beta Tubulin stained by beta Tubulin antibody (GTX101279) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-beta Tubulin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101279

GTX101279 ICC/IF Image

beta Tubulin antibody detects beta Tubulin protein at cytoskeleton by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: beta Tubulin stained by beta Tubulin antibody (GTX101279) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

文献引用

产品评论

beta Tubulin antibody 目录号 GTX101279

Rating ( Average 4.6 based on 5 users reviews)
实验应用
Western Blot(WB) ( Average 4.6 based on 5 users reviews)
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Cell Biosci in 2014. PMID: 25126410
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Cancer Metab in 2014. PMID: 25225614
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2018. PMID: 29950685
Date : Anonymous submitted on 04-Jun-2015
Score :
Application Tested : WB
Sample Species : Hu
Sample : Huh7 cells
Amount used : 20μg
Blocking : 5% skim milk in TBS, 25°C, 0.5Hr
Primary Antibody : 1:5000, 4°C, 16Hr
Date : Anonymous submitted on 04-Jun-2015
Score :
Application Tested : WB
Sample Species : Hu
Sample : MCF7 cells
Amount used : 20μg
Blocking : 5% skim milk in TBS, 25°C, 0.5Hr
Primary Antibody : 1:5000, 4°C, 16Hr
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
产品包装 报价 (¥)
¥ 3590
¥ 1390
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