beta Tubulin抗体

Name: beta Tubulin抗体
Brand: GeneTex
SKU: GTX101279
Price: 4000.00 RMB
Rating: 4 (2 reviews)

Anti-beta Tubulin antibody used in IHC (Paraffin sections) (IHC-P). GTX101279

Anti-beta Tubulin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101279

Anti-beta Tubulin antibody used in Western Blot (WB). GTX101279

Anti-beta Tubulin antibody used in IHC (Whole mount) (IHC-Wm). GTX101279

目录号 GTX101279

文献引用 35

宿主	Rabbit
克隆	Polyclonal
同种型	IgG
实验应用	WB, ICC/IF, IHC-P, IHC-Wm
种属反应	Human, Mouse, Rat, Zebrafish, Hamster
产品包装	100 μl (¥4000), 25 μl (¥1700)

查看所有 beta Tubulin 的产品

View product citations for antibody GTX101279 on CiteAb

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认

实验应用	稀释倍数
WB	1:500-1:20000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000
IHC-Wm	Assay dependent

WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

阳性对照

NIH-3T3 , 293T , A431 , HeLa , HepG2 , A549 , H1299 , HCT116

预测种属反应

Bovine, Chicken, Pig, Xenopus tropicalis, Chimpanzee, Rhesus Monkey(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1.35 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the center region of human beta Tubulin. The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_1952434

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

研究领域

產品图片

GTX101279 IHC-P Image

Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using beta Tubulin antibody (GTX101279) at 1:300 dilution.

GTX101279 ICC/IF Image

beta Tubulin antibody detects beta Tubulin protein at cytoskeleton by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: beta Tubulin stained by beta Tubulin antibody (GTX101279) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

GTX101279 WB Image

Various tissue extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Tubulin antibody (GTX101279) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101279 WB Image

GTX101279 IHC-Wm Image

beta Tubulin antibody detects beta Tubulin protein on zebrafish by whole mount immunohistochemical analysis.
Sample: 1 day-post-fertilization zebrafish embryo.
beta Tubulin antibody (GTX101279) dilution: 1:100.

GTX101279 IHC-P Image

beta Tubulin antibody detects beta Tubulin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse spinal cord.
beta Tubulin stained by beta Tubulin antibody (GTX101279) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX101279 IHC-P Image

beta Tubulin antibody detects beta Tubulin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
beta Tubulin stained by beta Tubulin antibody (GTX101279) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX101279 IHC-P Image

beta Tubulin antibody detects beta Tubulin protein at cytoplasm in rat spleen by immunohistochemical analysis.
Sample: Paraffin-embedded rat spleen.
beta Tubulin antibody (GTX101279) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX101279 IHC-P Image

beta Tubulin antibody detects beta Tubulin protein at cytoplasm in rat spinal cord by immunohistochemical analysis.
Sample: Paraffin-embedded rat spinal cord.
beta Tubulin antibody (GTX101279) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX101279 WB Image

Various whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with beta Tubulin antibody (GTX101279) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101279 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Tubulin antibody (GTX101279) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101279 IHC-P Image

beta Tubulin antibody detects beta Tubulin protein at cytoplasm on human breast cancer by immunohistochemical analysis.
Sample: Paraffin-embedded breast cancer.
beta Tubulin antibody (GTX101279) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101279 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using beta Tubulin(GTX101279) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

GTX101279 WB Image

Sample (30 μg of whole cell lysate)
A: NIH-3T3
10% SDS PAGE
GTX101279 diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101279 WB Image

beta Tubulin antibody detects beta Tubulin protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Tubulin antibody (GTX101279) diluted at a dilution of 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101279 WB Image

The data was published in the journal Sci Rep in 2018. PMID: 29950685

GTX101279 WB Image

The data was published in the journal Cell Biosci in 2014. PMID: 25126410

GTX101279 WB Image

The data was published in the journal Cancer Metab in 2014. PMID: 25225614

文献引用

Application Reference

M Viettri et al. Microbiol Spectr 2023; : e0063023 Comparative Infections of Zika, Dengue, and Yellow Fever Viruses in Human Cytotrophoblast-Derived Cells Suggest a Gating Role for the Cytotrophoblast in Zika Virus Placental Invasion

种属反应 : Mouse

Cheng H et al. Evid Based Complement Alternat Med 2023; 2023 : 8673136 Study on Neuroprotective Mechanism of Houshiheisan in Ischemic Stroke Based on Transcriptomics and Experimental Verification.

实验应用 : WB

种属反应 : Rat

Jiang B et al. Mol Med Rep 2023; 27 (2) Combination of chloroquine diphosphate and salidroside induces human liver cell apoptosis via regulation of mitochondrial dysfunction and autophagy.

Chen GT et al. Mol Cancer Res 2022; 20 (3) : 468-484 Disruption of β-Catenin-Dependent Wnt Signaling in Colon Cancer Cells Remodels the Microenvironment to Promote Tumor Invasion.

实验应用 : WB

种属反应 : Human

Venuti A et al. J Virol 2022; 96 (14) : e0206121 Lyon IARC Polyomavirus Displays Transforming Activities in Primary Human Cells.

实验应用 : WB

种属反应 : Human

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我要回报文献

产品评论

beta Tubulin antibody 目录号 GTX101279

Rating

( Average 4 based on 2 users reviews)

实验应用

Western Blot(WB)

( Average 4 based on 2 users reviews)

Date : Anonymous submitted on 04-Jun-2015

Score :

Application Tested : WB

Sample Species : Hu

Sample : Huh7 cells

Amount used : 20μg

Blocking : 5% skim milk in TBS, 25°C, 0.5Hr

Primary Antibody : 1:5000, 4°C, 16Hr

Date : Anonymous submitted on 04-Jun-2015

Score :

Application Tested : WB

Sample Species : Hu

Sample : MCF7 cells

Amount used : 20μg

Blocking : 5% skim milk in TBS, 25°C, 0.5Hr

Primary Antibody : 1:5000, 4°C, 16Hr

我要评论产品

Application Reference

M Viettri et al. Microbiol Spectr 2023;:e0063023 Comparative Infections of Zika, Dengue, and Yellow Fever Viruses in Human Cytotrophoblast-Derived Cells Suggest a Gating Role for the Cytotrophoblast in Zika Virus Placental Invasion

种属反应 : Mouse

Cheng H et al. Evid Based Complement Alternat Med 2023; 2023:8673136 Study on Neuroprotective Mechanism of Houshiheisan in Ischemic Stroke Based on Transcriptomics and Experimental Verification.

实验应用 : WB

种属反应 : Rat

Jiang B et al. Mol Med Rep 2023; 27 (2) Combination of chloroquine diphosphate and salidroside induces human liver cell apoptosis via regulation of mitochondrial dysfunction and autophagy.

Peng KL et al. Proc Natl Acad Sci U S A 2022; 119 (37):e2203738119 Miat and interacting protein Metadherin maintain a stem-like niche to promote medulloblastoma tumorigenesis and treatment resistance.

Chen GT et al. Mol Cancer Res 2022; 20 (3):468-484 Disruption of β-Catenin-Dependent Wnt Signaling in Colon Cancer Cells Remodels the Microenvironment to Promote Tumor Invasion.

实验应用 : WB

种属反应 : Human

Venuti A et al. J Virol 2022; 96 (14):e0206121 Lyon IARC Polyomavirus Displays Transforming Activities in Primary Human Cells.

实验应用 : WB

种属反应 : Human

Wen-Jui Lee et al. Molecules 2021; 26 (21) Type-3 Hyaluronan Synthase Attenuates Tumor Cells Invasion in Human Mammary Parenchymal Tissues.

实验应用 : ICC/IF

种属反应 : Human

Huang SS et al. Front Oncol 2021; 11:638311 A Novel Invadopodia-Specific Marker for Invasive and Pro-Metastatic Cancer Stem Cells.

实验应用 : WB

种属反应 : Human

Kim S et al. Cell Death Differ 2021; PARsylated transcription factor EB (TFEB) regulates the expression of a subset of Wnt target genes by forming a complex with β-catenin-TCF/LEF1.

实验应用 : WB

种属反应 : Human

Eller C et al. Nat Commun 2020; 11 (1):2707 A genome-wide gain-of-function screen identifies CDKN2C as a HBV host factor.

Huang HY et al. J Cell Mol Med 2020; Progesterone receptor membrane component 1 is involved in oral cancer cell metastasis.

实验应用 : WB

种属反应 : Human

George T. Chen et al. bioRxiv 2019; (Epub) Disrupting ?-catenin dependent Wnt signaling activates an invasive gene program predictive of colon cancer progression.

实验应用 : WB

种属反应 : Human

Ishida Y et al. J Interferon Cytokine Res 2019; 39 (3):133-146 Hepatic IFN-Induced Protein with Tetratricopeptide Repeats Regulation of HCV Infection.

实验应用 : WB

种属反应 : Human

Pai VC et al. Oncogene 2018; (Epub) ASPM promotes prostate cancer stemness and progression by augmenting Wnt-Dvl-3-β-catenin signaling.

实验应用 : WB

种属反应 : Human

Wakae K et al. Sci Rep 2018; 8 (1):9745 Keratinocyte differentiation induces APOBEC3A, 3B, and mitochondrial DNA hypermutation.

实验应用 : WB

种属反应 : Human

Lin M et al. Phytother Res 2018; (Epub) Quercetin improves postpartum hypogalactia in milk-deficient mice via stimulating prolactin production in pituitary gland.

实验应用 : WB

种属反应 : Mouse

Yoshimoto M et al. Oncology 2018; (Epub) Abnormal Expression of PICT-1 and Its Codon 389 Polymorphism Is a Risk Factor for Human Endometrial Cancer.

实验应用 : WB

种属反应 : Human

Wang F et al. Mol Cancer Ther 2018; 17 (4):763-775 SKLB-23bb, A HDAC6-Selective Inhibitor, Exhibits Superior and Broad-Spectrum Antitumor Activity via Additionally Targeting Microtubules.

实验应用 : WB

种属反应 : Human

Yu-Lee LY et al. Cancer Res 2018; (Epub) Osteoblast-secreted factors mediate dormancy of metastatic prostate cancer in the bone via activation of the TGFβRIII-p38MAPK-pS249/T252RB pathway.

实验应用 : WB

种属反应 : Human

Her LS et al. Theranostics 2017; 7 (9):2452-2462 miR-196a Enhances Neuronal Morphology through Suppressing RANBP10 to Provide Neuroprotection in Huntington's Disease.

实验应用 : ICC/IF

种属反应 : Mouse

Cheng JC et al. Sci Rep 2017; 7 (1):4448 Activation of hepatic stellate cells by the ubiquitin C-terminal hydrolase 1 protein secreted from hepatitis C virus-infected hepatocytes.

实验应用 : WB

种属反应 : Rat

Chiang WC et al. Proc Natl Acad Sci U S A 2017; 114 (2):400-405 Achromatopsia mutations target sequential steps of ATF6 activation.

实验应用 : WB

种属反应 : Human

Yoshimoto M et al. Mol Carcinog 2016; (Epub) The Protein Interacting with Carboxyl Terminus-1 codon 389 polymorphism impairs protein interacting with carboxyl terminus-1 function and is a risk factor for uterine cervical cancer.

Albert JS et al. Orphanet J Rare Dis 2015; 10:27 Impaired osteoblast and osteoclast function characterize the osteoporosis of Snyder - Robinson syndrome.

实验应用 : WB

种属反应 : Human

Hsiao WY et al. PLoS One 2015; 10 (12):e0145880 Dual-Specificity Phosphatase 4 Regulates STAT5 Protein Stability and Helper T Cell Polarization.

实验应用 : WB

种属反应 : Human

Kohl S et al. Nat Genet 2015; 47 (7):757-65 Mutations in the unfolded protein response regulator ATF6 cause the cone dysfunction disorder achromatopsia.

Lin YJ et al. Biomedicine (Taipei) 2015; 5 (2):10 P-coumaric acid regulates exon 12 splicing of the ATP7B gene by modulating hnRNP A1 protein expressions.

实验应用 : WB

种属反应 : Hamster

Murakami S et al. Neurochem Res 2015; 40 (6):1165-78 Long-Term Systemic Exposure to Rotenone Induces Central and Peripheral Pathology of Parkinson's Disease in Mice.

实验应用 : IHC-P

种属反应 : Mouse

Lee YZ et al. Oncotarget 2014; 5 (15):6087-101 Discovery of selective inhibitors of Glutaminase-2, which inhibit mTORC1, activate autophagy and inhibit proliferation in cancer cells.

实验应用 : WB

种属反应 : Human

Roy D et al. Cancer Metab 2014; 2:13 Loss of HSulf-1 promotes altered lipid metabolism in ovarian cancer.

实验应用 : WB

种属反应 : Human

Lin YJ et al. Cell Biosci 2014; 4:40 Ring finger protein 39 genetic variants associate with HIV-1 plasma viral loads and its replication in cell culture.

Law CH et al. Toxicology 2013; 303:54-71 Hyaluronic acid-dependent protection in H9C2 cardiomyocytes: a cell model of heart ischemia-reperfusion injury and treatment.

实验应用 : WB

种属反应 : Rat

Fan CY et al. Electrophoresis 2013; 34 (24):3305-14 Proteomic and redox-proteomic study on the role of glutathione reductase in human lung cancer cells.

实验应用 : WB

种属反应 : Human

Lin ST et al. J Proteomics 2012; 75 (18):5822-47 proteomic analysis of proteins responsible for the development of * resistance in human uterine cancer cells.

实验应用 : WB

种属反应 : Human

Cho CK et al. J Proteome Res 2010; 9 (7):3574-82 Amniotic fluid proteome analysis from Down syndrome pregnancies for biomarker discovery.

实验应用 : WB

种属反应 : Human

SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf

产品包装	报价 (¥)
100 μl	¥ 4000
25 μl	¥ 1700
Qty

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	http://www.neobioscience.com
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beta Tubulin抗体

目录号 GTX101279

宿主

克隆

同种型

实验应用

种属反应

产品应用

应用说明

阳性对照

预测种属反应

产品属性

形式

存储溶液

存放说明

浓度

免疫原种属

免疫原

纯化方式

偶联

RRID

注意事项

相关讯息

研究领域

產品图片

GTX101279 IHC-P Image

GTX101279 ICC/IF Image

GTX101279 WB Image

GTX101279 WB Image

GTX101279 IHC-Wm Image

GTX101279 IHC-P Image

GTX101279 IHC-P Image

GTX101279 IHC-P Image

GTX101279 IHC-P Image

GTX101279 WB Image

GTX101279 WB Image

GTX101279 IHC-P Image

GTX101279 ICC/IF Image

GTX101279 WB Image

GTX101279 WB Image

GTX101279 WB Image

GTX101279 WB Image

GTX101279 WB Image

文献引用

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产品评论

beta Tubulin antibody 目录号 GTX101279

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