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beta Tubulin抗体

Anti-beta Tubulin antibody used in IHC (Paraffin sections) (IHC-P). GTX107175
Anti-beta Tubulin antibody used in Western Blot (WB). GTX107175
Anti-beta Tubulin antibody used in Western Blot (WB). GTX107175
Anti-beta Tubulin antibody used in Western Blot (WB). GTX107175
Anti-beta Tubulin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107175
Anti-beta Tubulin antibody used in Western Blot (WB). GTX107175
Anti-beta Tubulin antibody used in Western Blot (WB). GTX107175

目录号 GTX107175

宿主

Rabbit

克隆

Polyclonal

同种型

IgG

实验应用

WB, ICC/IF, IHC-P, IHC-Fr, IP

种属反应

Human, Mouse, Zebrafish
产品包装
100 μl (¥4000),
25 μl (¥1700)

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P Assay dependent
IHC-Fr Assay dependent
IP Assay dependent
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

50 kDa. ( Note )

阳性对照

A431 , HepG2 , NIH-3T3

预测种属反应

Bovine, Cat, Chicken, Pig, Xenopus tropicalis, Chimpanzee, Rhesus Monkey(>80% identity)

产品属性

形式

Liquid

存储溶液

0.1M Tris, 0.1M Glycine, 10% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the center region of human beta Tubulin. The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_1952436

注意事项

仅供实验室使用。不适用于人类或动物的任何临床,治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

tubulin beta 1 class VI

功能与背景

Microtubules are involved in a wide variety of cellular processes, including mitosis, morphogenesis, platelet formation, and mobility of cilia and flagella. Circulating platelets carry a single marginal microtubule coil that is wound in 8 to 12 turns and is responsible for platelet shape. TUBB1 is the major beta-tubulin expressed in platelets and megakaryocytes and is required for optimal platelet assembly (Wang et al., 1986 [PubMed 3782288]; Schulze et al., 2004 [PubMed 15315966]).[supplied by OMIM]

数据库

研究领域

產品图片

Anti-beta Tubulin antibody used in IHC (Paraffin sections) (IHC-P). GTX107175

GTX107175 IHC-P Image

Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using beta Tubulin antibody (GTX107175) at 1:300 dilution.

Anti-beta Tubulin antibody used in Western Blot (WB). GTX107175

GTX107175 WB Image

Sample (30 μg of whole cell lysate)
A: Adult zebrafish
7.5% SDS PAGE
GTX107175 diluted at 1:1000

Anti-beta Tubulin antibody used in Western Blot (WB). GTX107175

GTX107175 WB Image

Sample(30 ug whole cell lysate)
A:A431(GTX27909)
B:Hep G2 (GTX27900)
10% SDS PAGE
GTX107175 diluted at 1:1000

Anti-beta Tubulin antibody used in Western Blot (WB). GTX107175

GTX107175 WB Image

Sample (30 ug of whole cell lysate)
A: NIH-3T3
10% SDS PAGE
GTX107175 diluted at 1:1000

Anti-beta Tubulin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107175

GTX107175 ICC/IF Image

beta Tubulin antibody detects beta Tubulin protein at cytoskeleton by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: beta Tubulin protein stained by beta Tubulin antibody (GTX107175) diluted at 1:200.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-beta Tubulin antibody used in Western Blot (WB). GTX107175

GTX107175 WB Image

The data was published in the journal Cancer Metab in 2016.PMID: 27729975

Anti-beta Tubulin antibody used in Western Blot (WB). GTX107175

GTX107175 WB Image

The data was published in the journal Open Biol in 2014. PMID: 25392452

文献引用

产品评论

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SDS
Glycerol.pdf
Thimerosal.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
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