产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:500-1:3000 |
| Assay dependent |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
产品说明
This antibody is raised against human c-Jun phosphorylated at Ser73. Based on sequence homology, it is predicted to react with human JunD phosphorylated at Ser100.
产品属性
形式
Liquid
存储溶液
PBS
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Carrier-protein conjugated synthetic peptide surrounding phospho Ser73 of human c-Jun. The exact sequence is proprietary.
纯化方式
Affinity purified by Protein A.
偶联
Unconjugated
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
Jun proto-oncogene, AP-1 transcription factor subunit , AP-1 , AP1 , c-Jun , p39
细胞定位
Nucleus
功能与背景
This gene is the putative transforming gene of avian sarcoma virus 17. It encodes a protein which is highly similar to the viral protein, and which interacts directly with specific target DNA sequences to regulate gene expression. This gene is intronless and is mapped to 1p32-p31, a chromosomal region involved in both translocations and deletions in human malignancies. [provided by RefSeq, Jul 2008]
数据库
研究领域
產品图片
![Anti-c-Jun (phospho Ser73) antibody [HL2713] used in Western Blot (WB). GTX639474](/templates/default/js/lightbox/images/loading.gif "Anti-c-Jun (phospho Ser73) antibody [HL2713] used in Western Blot (WB). GTX639474")
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GTX639474 WB Image
Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with c-Jun (phospho Ser73) antibody [HL2713] (GTX639474) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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GTX639474 ICC/IF Image
c-Jun (phospho Ser73) antibody [HL2713] detects c-Jun (phospho Ser73) protein at nucleus by immunofluorescent analysis.Sample: Mock and treated HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: c-Jun (phospho Ser73) stained by c-Jun (phospho Ser73) antibody [HL2713] (GTX639474) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.Blue: Fluoroshield with DAPI (GTX30920).
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文献引用
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产品评论
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![Anti-c-Jun (phospho Ser73) antibody [HL2713] used in Western Blot (WB). GTX639474](/upload/website/prouct_img/small/GTX639474/GTX639474_45313_20240216_WB_treatment_anisomycin_24022619_627.webp "Anti-c-Jun (phospho Ser73) antibody [HL2713] used in Western Blot (WB). GTX639474")
![Anti-c-Jun (phospho Ser73) antibody [HL2713] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX639474](/upload/website/prouct_img/small/GTX639474/GTX639474_T-45250_20240126_ICC_IF_treatment_anisomycin_24021917_723.webp "Anti-c-Jun (phospho Ser73) antibody [HL2713] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX639474")
![Anti-c-Jun (phospho Ser73) antibody [HL2713] used in Western Blot (WB). GTX639474](/upload/website/prouct_img/normal/GTX639474/GTX639474_45313_20240216_WB_treatment_anisomycin_24022619_627.webp "Anti-c-Jun (phospho Ser73) antibody [HL2713] used in Western Blot (WB). GTX639474")
![Anti-c-Jun (phospho Ser73) antibody [HL2713] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX639474](/upload/website/prouct_img/normal/GTX639474/GTX639474_T-45250_20240126_ICC_IF_treatment_anisomycin_24021917_723.webp "Anti-c-Jun (phospho Ser73) antibody [HL2713] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX639474")
