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c-Met抗体 [C3], C-term

KO/KD
Anti-c-Met antibody [C3], C-term used in Western Blot (WB). GTX100637
Anti-c-Met antibody [C3], C-term used in Western Blot (WB). GTX100637
Anti-c-Met antibody [C3], C-term used in Immunoprecipitation (IP). GTX100637
Anti-c-Met antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100637
Anti-c-Met antibody [C3], C-term used in Western Blot (WB). GTX100637
Anti-c-Met antibody [C3], C-term used in Western Blot (WB). GTX100637

目录号 GTX100637

文献引用 7

宿主

 Rabbit

克隆

 Polyclonal

同种型

 IgG

实验应用

 WB, ICC/IF, IP

种属反应

 Human
产品包装
100 μl (¥4000),
25 μl (¥1700)
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产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IP 1:100-1:500
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

156 kDa. ( Note )

阳性对照

HeLa

预测种属反应

Chimpanzee(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1.95 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Synthetic peptide encompassing a sequence within the Intracellular domain of human c-Met. The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_1950910

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

MET proto-oncogene, receptor tyrosine kinase , AUTS9 , DFNB97 , HGFR , RCCP2 , c-Met

细胞定位

Membrane; Single-pass type I membrane protein

功能与背景

The proto-oncogene MET product is the hepatocyte growth factor receptor and encodes tyrosine-kinase activity. The primary single chain precursor protein is post-translationally cleaved to produce the alpha and beta subunits, which are disulfide linked to form the mature receptor. Various mutations in the MET gene are associated with papillary renal carcinoma. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

数据库

研究领域

產品图片

Anti-c-Met antibody [C3], C-term used in Western Blot (WB). GTX100637

GTX100637 WB Image

KO/KD

Wild-type (WT) and c-Met knockout (KO) HeLa cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with c-Met antibody [C3], C-term (GTX100637) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-c-Met antibody [C3], C-term used in Western Blot (WB). GTX100637

GTX100637 WB Image

KO/KD

Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with c-Met antibody [C3], C-term (GTX100637) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-c-Met antibody [C3], C-term used in Immunoprecipitation (IP). GTX100637

GTX100637 IP Image

Immunoprecipitation of c-Met protein from HeLa whole cell extracts using 5 μg of c-Met antibody [C3], C-term (GTX100637).
Western blot analysis was performed using c-Met antibody [C3], C-term (GTX100637) diluted at 1:500.
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-c-Met antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100637

GTX100637 ICC/IF Image

c-Met antibody [c3], c-term detects c-Met protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: c-Met protein stained by c-Met antibody [c3], c-term (GTX100637) diluted at 1:1000.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-c-Met antibody [C3], C-term used in Western Blot (WB). GTX100637

GTX100637 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with c-Met antibody [C3], C-term (GTX100637) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-c-Met antibody [C3], C-term used in Western Blot (WB). GTX100637

GTX100637 WB Image

The data was published in the journal Oncogene in 2018. PMID: 29993037

文献引用

Application Reference

Elisa De Tomi et al. Int J Mol Sci 2022; 23 (3) Upregulation of miR-34a-5p, miR-20a-3p and miR-29a-3p by Onconase in A375 Melanoma Cells Correlates with the Downregulation of Specific Onco-Proteins.
实验应用 : WB
种属反应 : Human
Pang HH et al. Nanoscale 2019; 11 (17) : 8102-8109 Bioengineering fluorescent virus-like particle/RNAi nanocomplexes act synergistically with temozolomide to eradicate brain tumors.
实验应用 : WB
种属反应 : Human
Chen ST et al. Oncogene 2018; (Epub) Silencing of MUC20 suppresses the malignant character of pancreatic ductal adenocarcinoma cells through inhibition of the HGF/MET pathway.
实验应用 : WB
种属反应 : Human
Pei YF et al. Biochim Biophys Acta 2017; (Epub) TOP2A induces malignant character of pancreatic cancer through activating β-catenin signaling pathway.
实验应用 : WB
种属反应 : Human
Wang C et al. Exp Dermatol 2014; 23 (11) : 843-9 Loss of cell invasiveness through PKC-mediated syndecan-1 downregulation in melanoma cells under anchorage independency.
实验应用 : WB
种属反应 : Human
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产品包装 报价 (¥)
¥ 4000
¥ 1700
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