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c-Met抗体 [GT556]

KO/KD
Anti-c-Met antibody [GT556] used in Western Blot (WB). GTX631992
Anti-c-Met antibody [GT556] used in Western Blot (WB). GTX631992
Anti-c-Met antibody [GT556] used in IHC (Paraffin sections) (IHC-P). GTX631992
Anti-c-Met antibody [GT556] used in Immunoprecipitation (IP). GTX631992
Anti-c-Met antibody [GT556] used in Western Blot (WB). GTX631992
Anti-c-Met antibody [GT556] used in IHC (Paraffin sections) (IHC-P). GTX631992

目录号 GTX631992

文献引用 2
1

宿主

 Mouse

克隆

 Monoclonal

克隆编号

 GT556

同种型

 IgG2a

实验应用

 WB, IHC-P, FACS, IP

种属反应

 Human
产品包装
100 μl (¥4000),
25 μl (¥1700)
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产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
IHC-P 1:100-1:1000
FACS Assay dependent
IP 1:100-1:500
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

156 kDa. ( Note )

阳性对照

A431 , HeLa , HepG2

产品说明

This antibody was raised against human c-Met Intracellular domain.

预测种属反应

Chimpanzee(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Synthetic peptide encompassing a sequence within the Intracellular domain of human c-Met. The exact sequence is proprietary.

纯化方式

Affinity purified by Protein A.

偶联

Unconjugated

RRID

AB_2888270

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

MET proto-oncogene, receptor tyrosine kinase , AUTS9 , DFNB97 , HGFR , RCCP2 , c-Met

细胞定位

Membrane; Single-pass type I membrane protein

功能与背景

The proto-oncogene MET product is the hepatocyte growth factor receptor and encodes tyrosine-kinase activity. The primary single chain precursor protein is post-translationally cleaved to produce the alpha and beta subunits, which are disulfide linked to form the mature receptor. Various mutations in the MET gene are associated with papillary renal carcinoma. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

数据库

研究领域

產品图片

Anti-c-Met antibody [GT556] used in Western Blot (WB). GTX631992

GTX631992 WB Image

KO/KD

Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with c-Met antibody [GT556] (GTX631992) diluted at 1:500.

Anti-c-Met antibody [GT556] used in Western Blot (WB). GTX631992

GTX631992 WB Image

KO/KD

Wild-type (WT) and c-Met knockout (KO) HeLa cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with c-Met antibody [GT556] (GTX631992) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-c-Met antibody [GT556] used in IHC (Paraffin sections) (IHC-P). GTX631992

GTX631992 IHC-P Image

c-Met antibody [GT556] detects c-Met protein at cytoplasm in human esophageal carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human esophageal carcinoma.
c-Met antibody [GT556] (GTX631992) diluted at 1:100.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-c-Met antibody [GT556] used in Immunoprecipitation (IP). GTX631992

GTX631992 IP Image

Immunoprecipitation of c-Met protein from HeLa whole cell extracts using 5 μg of MET antibody [GT556] (GTX631992).
Western blot analysis was performed using c-Met antibody [GT556] (GTX631992).
EasyBlot anti-Mouse IgG (GTX221667-01) was used as a secondary reagent.

Anti-c-Met antibody [GT556] used in Western Blot (WB). GTX631992

GTX631992 WB Image

c-Met antibody detects c-Met protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with c-Met antibody (GTX631992) diluted by 1:1000.

Anti-c-Met antibody [GT556] used in IHC (Paraffin sections) (IHC-P). GTX631992

GTX631992 IHC-P Image

c-Met antibody [GT556] detects c-Met protein at cytoplasm in human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
c-Met antibody [GT556] (GTX631992) diluted at 1:100.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

文献引用

Application Reference

Sun Z et al. Exp Ther Med 2024; 27 (4) : 156 Predominant control of PDGF/PDGF receptor signaling in the migration and proliferation of human adipose?derived stem cells under culture conditions with a combination of growth factors.
Hung YH et al. Am J Cancer Res 2022; 12 (2) : 713-728 Semaphorin 4C promotes motility and immunosuppressive activity of cancer cells via CRMP3 and PD-L1.
实验应用 : FACS
种属反应 : Human
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产品评论

c-Met antibody [GT556] 目录号 GTX631992

Rating
( Average 5 based on 1 users reviews)
实验应用
IHC (Paraffin sections)(IHC-P)
( Average 5 based on 1 users reviews)
Anti-c-Met antibody [GT556] used in IHC (Paraffin sections) (IHC-P). GTX631992
Date : Anonymous submitted on 21-Oct-2020
Score :
Application Tested : IHC-P
Sample Species : Hu
Sample : liver cancer tissue
Fixation : Citrate buffer, 0.3°C
Primary Antibody : 1:400, 4°C, 16Hr
我要评论产品
SDS
PBS.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
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