产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
实验应用 |
稀释倍数 |
1:500-1:3000 |
Assay dependent |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
预测种属反应
Rhesus Monkey(>80% identity)
产品属性
形式
Liquid
存储溶液
PBS
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant protein encompassing a sequence within the center region of human cIAP1. The exact sequence is proprietary.
纯化方式
Affinity purified by Protein A.
偶联
Unconjugated
RRID
AB_2909931
注意事项
仅供实验室使用。不适用于人类或动物的任何临床,治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
baculoviral IAP repeat containing 2 , API1 , HIAP2 , Hiap-2 , MIHB , RNF48 , c-IAP1 , cIAP1
细胞定位
Cytoplasm,Nucleus
功能与背景
The protein encoded by this gene is a member of a family of proteins that inhibits apoptosis by binding to tumor necrosis factor receptor-associated factors TRAF1 and TRAF2, probably by interfering with activation of ICE-like proteases. This encoded protein inhibits apoptosis induced by serum deprivation and menadione, a potent inducer of free radicals. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]
数据库
研究领域
產品图片
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GTX635979 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with cIAP1 antibody [HL1045] (GTX635979) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX635979 WB Image
Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membranes were blotted with cIAP1 antibody [HL1045] (GTX635979) diluted at 1:1000 and competitor's antibody (Competitor) diluted at 1:100. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.
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GTX635979 IHC-P Image
cIAP1 antibody [HL1045] detects cIAP1 protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse tissues.cIAP1 stained by cIAP1 antibody [HL1045] (GTX635979) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX635979 IHC-P Image
cIAP1 antibody [HL1045] detects cIAP1 protein at cytoplasm and nucleus by immunohistochemical analysis.Sample: Paraffin-embedded rat spleen.cIAP1 stained by cIAP1 antibody [HL1045] (GTX635979) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX635979 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with cIAP1 antibody [HL1045] (GTX635979) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX635979 WB Image
Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with cIAP1 antibody [HL1045] (GTX635979) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX635979 IHC-P Image
cIAP1 antibody [HL1045] detects cIAP1 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded dog pancreas.cIAP1 stained by cIAP1 antibody [HL1045] (GTX635979) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX635979 IHC-P Image
cIAP1 antibody [HL1045] detects cIAP1 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded cat lung.cIAP1 stained by cIAP1 antibody [HL1045] (GTX635979) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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产品评论
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