产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| Assay dependent |
| 1:100-1:1000 |
| Assay dependent |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
产品属性
形式
Liquid
存储溶液
PBS
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant protein encompassing a sequence within the center region of human eIF4E. The exact sequence is proprietary.
纯化方式
Affinity purified by Protein A.
偶联
Unconjugated
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
eukaryotic translation initiation factor 4E , AUTS19 , CBP , EIF4E1 , EIF4EL1 , EIF4F , eIF-4E
细胞定位
Cytoplasm
功能与背景
The protein encoded by this gene is a component of the eukaryotic translation initiation factor 4F complex, which recognizes the 7-methylguanosine cap structure at the 5' end of messenger RNAs. The encoded protein aids in translation initiation by recruiting ribosomes to the 5'-cap structure. Association of this protein with the 4F complex is the rate-limiting step in translation initiation. This gene acts as a proto-oncogene, and its expression and activation is associated with transformation and tumorigenesis. Several pseudogenes of this gene are found on other chromosomes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]
数据库
研究领域
產品图片
![Anti-eIF4E antibody [HL1553] used in Western Blot (WB). GTX637028](/templates/default/js/lightbox/images/loading.gif "Anti-eIF4E antibody [HL1553] used in Western Blot (WB). GTX637028")
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GTX637028 WB Image
293T whole cell and cytoplasm extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with eIF4E antibody [HL1553] (GTX637028) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX637028 IHC-P Image
eIF4E antibody [HL1553] detects eIF4E protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse kidney.eIF4E stained by eIF4E antibody [HL1553] (GTX637028) diluted at 1:100.Antigen Retrieval: Tris-EDTA buffer, pH 8.0, 15 min
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GTX637028 WB Image
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with eIF4E antibody [HL1553] (GTX637028) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX637028 IHC-P Image
eIF4E antibody [HL1553] detects eIF4E protein by immunohistochemical analysis.Sample: Paraffin-embedded rat tissues.eIF4E stained by eIF4E antibody [HL1553] (GTX637028) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX637028 ICC/IF Image
eIF4E antibody [HL1553] detects eIF4E protein at cytoplasm by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: eIF4E stained by eIF4E antibody [HL1553] (GTX637028) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.Blue: Fluoroshield with DAPI (GTX30920).
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GTX637028 WB Image
Mouse tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with eIF4E antibody [HL1553] (GTX637028) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
|
|
|
GTX637028 WB Image
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with eIF4E antibody [HL1553] (GTX637028) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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文献引用
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产品评论
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![Anti-eIF4E antibody [HL1553] used in Western Blot (WB). GTX637028](/upload/website/prouct_img/small/GTX637028/GTX637028_44781_20230728_WB_Fraction_23073119_837.webp "Anti-eIF4E antibody [HL1553] used in Western Blot (WB). GTX637028")
![Anti-eIF4E antibody [HL1553] used in IHC (Paraffin sections) (IHC-P). GTX637028](/upload/website/prouct_img/small/GTX637028/GTX637028_T-44697_20220729_IHC-P_M_22081423_667.webp "Anti-eIF4E antibody [HL1553] used in IHC (Paraffin sections) (IHC-P). GTX637028")
![Anti-eIF4E antibody [HL1553] used in Western Blot (WB). GTX637028](/upload/website/prouct_img/small/GTX637028/GTX637028_44781_20220826_WB_22083119_286.webp "Anti-eIF4E antibody [HL1553] used in Western Blot (WB). GTX637028")
![Anti-eIF4E antibody [HL1553] used in IHC (Paraffin sections) (IHC-P). GTX637028](/upload/website/prouct_img/small/GTX637028/GTX637028_44781_20221227_IHC-P_multiple_R_22122821_181.webp "Anti-eIF4E antibody [HL1553] used in IHC (Paraffin sections) (IHC-P). GTX637028")
![Anti-eIF4E antibody [HL1553] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637028](/upload/website/prouct_img/small/GTX637028/GTX637028_T-44697_20220624_ICC_IF_22071401_367.webp "Anti-eIF4E antibody [HL1553] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637028")
![Anti-eIF4E antibody [HL1553] used in Western Blot (WB). GTX637028](/upload/website/prouct_img/small/GTX637028/GTX637028_T-44697_20220715_WB_M_testis_22071823_262.webp "Anti-eIF4E antibody [HL1553] used in Western Blot (WB). GTX637028")
![Anti-eIF4E antibody [HL1553] used in Western Blot (WB). GTX637028](/upload/website/prouct_img/small/GTX637028/GTX637028_T-44697_20220715_WB_M_R_22071823_579.webp "Anti-eIF4E antibody [HL1553] used in Western Blot (WB). GTX637028")
![Anti-eIF4E antibody [HL1553] used in Western Blot (WB). GTX637028](/upload/website/prouct_img/normal/GTX637028/GTX637028_44781_20230728_WB_Fraction_23073119_837.webp "Anti-eIF4E antibody [HL1553] used in Western Blot (WB). GTX637028")
![Anti-eIF4E antibody [HL1553] used in IHC (Paraffin sections) (IHC-P). GTX637028](/upload/website/prouct_img/normal/GTX637028/GTX637028_T-44697_20220729_IHC-P_M_22081423_667.webp "Anti-eIF4E antibody [HL1553] used in IHC (Paraffin sections) (IHC-P). GTX637028")
![Anti-eIF4E antibody [HL1553] used in Western Blot (WB). GTX637028](/upload/website/prouct_img/normal/GTX637028/GTX637028_44781_20220826_WB_22083119_286.webp "Anti-eIF4E antibody [HL1553] used in Western Blot (WB). GTX637028")
![Anti-eIF4E antibody [HL1553] used in IHC (Paraffin sections) (IHC-P). GTX637028](/upload/website/prouct_img/normal/GTX637028/GTX637028_44781_20221227_IHC-P_multiple_R_22122821_181.webp "Anti-eIF4E antibody [HL1553] used in IHC (Paraffin sections) (IHC-P). GTX637028")
![Anti-eIF4E antibody [HL1553] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637028](/upload/website/prouct_img/normal/GTX637028/GTX637028_T-44697_20220624_ICC_IF_22071401_367.webp "Anti-eIF4E antibody [HL1553] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637028")
![Anti-eIF4E antibody [HL1553] used in Western Blot (WB). GTX637028](/upload/website/prouct_img/normal/GTX637028/GTX637028_T-44697_20220715_WB_M_testis_22071823_262.webp "Anti-eIF4E antibody [HL1553] used in Western Blot (WB). GTX637028")
![Anti-eIF4E antibody [HL1553] used in Western Blot (WB). GTX637028](/upload/website/prouct_img/normal/GTX637028/GTX637028_T-44697_20220715_WB_M_R_22071823_579.webp "Anti-eIF4E antibody [HL1553] used in Western Blot (WB). GTX637028")
