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gamma Synuclein抗体

KO/KD Orthogonal
Anti-gamma Synuclein antibody used in Western Blot (WB). GTX110483
Anti-gamma Synuclein antibody used in Western Blot (WB). GTX110483
Anti-gamma Synuclein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110483
Anti-gamma Synuclein antibody used in IHC (Paraffin sections) (IHC-P). GTX110483
Anti-gamma Synuclein antibody used in IHC (Frozen sections) (IHC-Fr). GTX110483
Anti-gamma Synuclein antibody used in IHC (Frozen sections) (IHC-Fr). GTX110483

目录号 GTX110483

文献引用 5

宿主

 Rabbit

克隆

 Polyclonal

同种型

 IgG

实验应用

 WB, ICC/IF, IHC-P, IHC-Fr, IHC

种属反应

 Human, Mouse
产品包装
100 μl (¥4000),
25 μl (¥1700)
查看所有 gamma Synuclein 的产品

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
IHC Assay dependent
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

13 kDa. ( Note )

阳性对照

HeLa

预测种属反应

Rhesus Monkey(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

0.83 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human gamma Synuclein. The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_1951959

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

synuclein gamma , BCSG1 , SR

细胞定位

Cytoplasm , perinuclear region , Centrosome , Spindle

功能与背景

Summary: This gene encodes a member of the synuclein family of proteins which are believed to be involved in the pathogenesis of neurodegenerative diseases. Mutations in this gene have also been associated with breast tumor development. [provided by RefSeq]

数据库

研究领域

產品图片

Anti-gamma Synuclein antibody used in Western Blot (WB). GTX110483

GTX110483 WB Image

KO/KD

Wild-type (WT) and gamma Synuclein knockout (KO) HeLa cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with gamma Synuclein antibody (GTX110483) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-gamma Synuclein antibody used in Western Blot (WB). GTX110483

GTX110483 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with gamma Synuclein antibody (GTX110483) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-gamma Synuclein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110483

GTX110483 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using gamma Synuclein (GTX110483) antibody at 1:200 dilution.

Anti-gamma Synuclein antibody used in IHC (Paraffin sections) (IHC-P). GTX110483

GTX110483 IHC-P Image

gamma Synuclein antibody detects gamma Synuclein protein at cytosol on mouse fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse fore brain.
gamma Synuclein antibody (GTX110483) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-gamma Synuclein antibody used in IHC (Frozen sections) (IHC-Fr). GTX110483

GTX110483 IHC-Fr Image

The data was published in the journal Mol Neurodegener in 2019.PMID: 31775817

Anti-gamma Synuclein antibody used in IHC (Frozen sections) (IHC-Fr). GTX110483

GTX110483 IHC-Fr Image

The data was published in the journal Sci Rep in 2017.PMID: 29196712

文献引用

Application Reference

Hsueh HT et al. Pharmaceutics 2021; 13 (5) Ion-Complex Microcrystal Formulation Provides Sustained Delivery of a Multimodal Kinase Inhibitor from the Subconjunctival Space for Protection of Retinal Ganglion Cells.
Xiao D et al. Sci Adv 2020; 6 (22) : eaaz5858 Generation of self-organized sensory ganglion organoids and retinal ganglion cells from fibroblasts.
实验应用 : IHC-P
种属反应 : Mouse
Welsbie DS et al. Mol Neurodegener 2019; 14 (1) : 44 Targeted disruption of dual leucine zipper kinase and leucine zipper kinase promotes neuronal survival in a model of diffuse traumatic brain injury.
实验应用 : IHC-P
种属反应 : Mouse
Yokoi T et al. Sci Rep 2017; 7 (1) : 16757 Effects of neuroactive agents on axonal growth and pathfinding of retinal ganglion cells generated from human stem cells.
实验应用 : IHC-Fr
种属反应 : Human
Chintalapudi SR et al. Front Aging Neurosci 2016; 8 : 93 Isolation and Molecular Profiling of Primary Mouse Retinal Ganglion Cells: Comparison of Phenotypes from Healthy and Glaucomatous Retinas.
实验应用 : ICC/IF, IHC
种属反应 : Mouse
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SDS
Glycerol.pdf
Thimerosal.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
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