产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:1000 |
| 1:50 |
| 1:200 |
| Assay dependent |
| Assay dependent |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
产品属性
形式
Liquid
存储溶液
PBS, 0.1% BSA
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant protein containing residues A A T R P D Y I S W G T Q E Q of human gamma Tubulin.
纯化方式
Protein G purified
偶联
Unconjugated
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
tubulin gamma 1 , CDCBM4 , GCP-1 , TUBG , TUBGCP1
功能与背景
This gene encodes a member of the tubulin superfamily. The encoded protein localizes to the centrosome where it binds to microtubules as part of a complex referred to as the gamma-tubulin ring complex. The protein mediates microtubule nucleation and is required for microtubule formation and progression of the cell cycle. A pseudogene of this gene is found on chromosome 7. [provided by RefSeq, Jan 2009]
数据库
研究领域
產品图片
![Anti-gamma Tubulin antibody [4D11] used in Western Blot (WB). GTX15794](/templates/default/js/lightbox/images/loading.gif "Anti-gamma Tubulin antibody [4D11] used in Western Blot (WB). GTX15794")
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GTX15794 WB Image
WB analysis of gamma tubulin knockdown HeLa cells (lane 3), scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1) using GTX15794 gamma Tubulin antibody [4D11].
Dilution : 1:1000
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GTX15794 IHC-P Image
IHC-P analysis of human colon tissue using GTX15794 gamma Tubulin antibody [4D11].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:200
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GTX15794 WB Image
WB analysis of 50 ug of various whole cell lysates using GTX15794 gamma Tubulin antibody [4D11].
Dilution : 1:1000
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GTX15794 WB Image
WB analysis of membrane enriched extracts (30 ug lysate) of A-549 (Lane 1), MCF-7 (Lane 2), HeLa (Lane 3), HepG2 (Lane 4), Caco2 (Lane 5), A-431 (Lane 6), SH-SY5Y (Lane 7) and U-2 OS (Lane 8) using GTX15794 gamma Tubulin antibody [4D11].
Dilution : 1 μg/ml
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GTX15794 ICC/IF Image
ICC/IF analysis of HeLa (A) and U2-OS cells (B) using GTX15794 gamma Tubulin antibody [4D11].
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes
Dilution : 1:50 for at least 1 hour at room temperature
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GTX15794 IHC-P Image
IHC-P analysis of human breast carcinoma tissue using GTX15794 gamma Tubulin antibody [4D11].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:20
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文献引用
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![Anti-gamma Tubulin antibody [4D11] used in Western Blot (WB). GTX15794](/upload/website/prouct_img/small/GTX15794/GTX15794_1539_WB_w_23060620_798.webp "Anti-gamma Tubulin antibody [4D11] used in Western Blot (WB). GTX15794")
![Anti-gamma Tubulin antibody [4D11] used in IHC (Paraffin sections) (IHC-P). GTX15794](/upload/website/prouct_img/small/GTX15794/GTX15794_1045_IHC-P_w_23060620_736.webp "Anti-gamma Tubulin antibody [4D11] used in IHC (Paraffin sections) (IHC-P). GTX15794")
![Anti-gamma Tubulin antibody [4D11] used in Western Blot (WB). GTX15794](/upload/website/prouct_img/small/GTX15794/GTX15794_1540_WB_w_23060620_499.webp "Anti-gamma Tubulin antibody [4D11] used in Western Blot (WB). GTX15794")
![Anti-gamma Tubulin antibody [4D11] used in Western Blot (WB). GTX15794](/upload/website/prouct_img/small/GTX15794/GTX15794_1541_WB_w_23060620_376.webp "Anti-gamma Tubulin antibody [4D11] used in Western Blot (WB). GTX15794")
![Anti-gamma Tubulin antibody [4D11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX15794](/upload/website/prouct_img/small/GTX15794/GTX15794_319_ICC-IF_w_23060620_584.webp "Anti-gamma Tubulin antibody [4D11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX15794")
![Anti-gamma Tubulin antibody [4D11] used in IHC (Paraffin sections) (IHC-P). GTX15794](/upload/website/prouct_img/small/GTX15794/GTX15794_1044_IHC-P_w_23060620_620.webp "Anti-gamma Tubulin antibody [4D11] used in IHC (Paraffin sections) (IHC-P). GTX15794")
![Anti-gamma Tubulin antibody [4D11] used in Western Blot (WB). GTX15794](/upload/website/prouct_img/normal/GTX15794/GTX15794_1539_WB_w_23060620_798.webp "Anti-gamma Tubulin antibody [4D11] used in Western Blot (WB). GTX15794")
![Anti-gamma Tubulin antibody [4D11] used in IHC (Paraffin sections) (IHC-P). GTX15794](/upload/website/prouct_img/normal/GTX15794/GTX15794_1045_IHC-P_w_23060620_736.webp "Anti-gamma Tubulin antibody [4D11] used in IHC (Paraffin sections) (IHC-P). GTX15794")
![Anti-gamma Tubulin antibody [4D11] used in Western Blot (WB). GTX15794](/upload/website/prouct_img/normal/GTX15794/GTX15794_1540_WB_w_23060620_499.webp "Anti-gamma Tubulin antibody [4D11] used in Western Blot (WB). GTX15794")
![Anti-gamma Tubulin antibody [4D11] used in Western Blot (WB). GTX15794](/upload/website/prouct_img/normal/GTX15794/GTX15794_1541_WB_w_23060620_376.webp "Anti-gamma Tubulin antibody [4D11] used in Western Blot (WB). GTX15794")
![Anti-gamma Tubulin antibody [4D11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX15794](/upload/website/prouct_img/normal/GTX15794/GTX15794_319_ICC-IF_w_23060620_584.webp "Anti-gamma Tubulin antibody [4D11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX15794")
![Anti-gamma Tubulin antibody [4D11] used in IHC (Paraffin sections) (IHC-P). GTX15794](/upload/website/prouct_img/normal/GTX15794/GTX15794_1044_IHC-P_w_23060620_620.webp "Anti-gamma Tubulin antibody [4D11] used in IHC (Paraffin sections) (IHC-P). GTX15794")
