产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:500-1:10000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
阳性对照
HeLa , 293T
产品属性
形式
Liquid
存储溶液
0.1M Tris, 0.1M Glycine, 20% Glycerol
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
0.75 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant protein encompassing a sequence within the center region of human hnRNP A2B1. The exact sequence is proprietary.
纯化方式
Purified by antigen-affinity chromatography.
偶联
Unconjugated
RRID
AB_11165592
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
heterogeneous nuclear ribonucleoprotein A2/B1 , HNRNPA2 , HNRNPB1 , HNRPA2 , HNRPA2B1 , HNRPB1 , IBMPFD2 , RNPA2 , SNRPB1
细胞定位
Nucleus , nucleoplasm , Cytoplasm
功能与背景
This gene belongs to the A/B subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has two repeats of quasi-RRM domains that bind to RNAs. This gene has been described to generate two alternatively spliced transcript variants which encode different isoforms. [provided by RefSeq]
数据库
研究领域
產品图片
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GTX114475 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with hnRNP A2B1 antibody (GTX114475) diluted at 1:2000.
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GTX114475 WB Image
Wild-type (WT) and hnRNP A2B1 knockout (KO) 293T cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with hnRNP A2B1 antibody (GTX114475) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX114475 ICC/IF Image
hnRNP A2B1 antibody detects hnRNP A2B1 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: hnRNP A2B1 protein stained by hnRNP A2B1 antibody (GTX114475) diluted at 1:1000.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.
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GTX114475 WB Image
Sample (30 ug of whole cell lysate)
A: HeLa
12% SDS PAGE
GTX114475 diluted at 1:5000
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GTX114475 IHC-P Image
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using hnRNP A2B1(GTX114475) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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文献引用
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