产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
实验应用 |
稀释倍数 |
1:500 - 1:2000 |
1:50 - 1:200 |
1:50 - 1:200 |
1:50 - 1:200 |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
Observed MW
120 kDa. The observed M.W. is based on the following publication. PMID: 22345668
产品属性
形式
Liquid
存储溶液
PBS, 0.05% BSA, 50% Glycerol
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, we recommend adding 50% glycerol, then aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
0.15 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
A synthesized peptide derived from human hnRNP U
纯化方式
Purified by antigen-affinity chromatography
偶联
Unconjugated
注意事项
仅供实验室使用。不适用于人类或动物的任何临床,治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
heterogeneous nuclear ribonucleoprotein U , EIEE54 , GRIP120 , HNRNPU-AS1 , HNRPU , SAF-A , SAFA , U21.1 , hnRNP U , pp120
细胞定位
Nucleus , Nucleus speckle , Cytoplasm
功能与背景
This gene encodes a member of a family of proteins that bind nucleic acids and function in the formation of ribonucleoprotein complexes in the nucleus with heterogeneous nuclear RNA (hnRNA). The encoded protein has affinity for both RNA and DNA, and binds scaffold-attached region (SAR) DNA. Mutations in this gene have been associated with epileptic encephalopathy, early infantile, 54. A pseudogene of this gene has been identified on chromosome 14. [provided by RefSeq, Jun 2017]
数据库
研究领域
產品图片
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GTX02839 WB Image
Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with hnRNP U antibody [GT1242] (GTX02839) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The observed M.W. is based on the publication: PMID: 22345668
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GTX02839 WB Image
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with hnRNP U antibody [GT1242] (GTX02839) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The observed M.W. is based on the publication: PMID: 22345668
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GTX02839 ICC/IF Image
ICC/IF analysis of C6 cells using GTX02839 hnRNP U antibody [GT1242]. Dilution : 1:100 Blue : DAPI for nuclear staining.
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GTX02839 IHC-P Image
IHC-P analysis of rat liver tissue section using GTX02839 hnRNP U antibody [GT1242]. Dilution : 1:100
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GTX02839 IP Image
IP analysis of K562 whole cell lysate using GTX02839 hnRNP U antibody [GT1242]. Total extract : 300μg IP antibody amount : 3μg Dilution : 1:1000
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GTX02839 IHC-P Image
IHC-P analysis of mouse liver tissue section using GTX02839 hnRNP U antibody [GT1242]. Dilution : 1:100
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GTX02839 ICC/IF Image
ICC/IF analysis of U2OS cells using GTX02839 hnRNP U antibody [GT1242]. Dilution : 1:100 Blue : DAPI for nuclear staining.
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GTX02839 WB Image
WB analysis of various samples using GTX02839 hnRNP U antibody [GT1242]. Dilution : 1:1000 Loading : 25μg
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GTX02839 IHC-P Image
IHC-P analysis of human thyroid cancer section using GTX02839 hnRNP U antibody [GT1242]. Dilution : 1:100
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GTX02839 ICC/IF Image
ICC/IF analysis of NIH-3T3 cells using GTX02839 hnRNP U antibody [GT1242]. Dilution : 1:100 Blue : DAPI for nuclear staining.
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文献引用
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产品评论
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