产品说明
摘要
mTOR antibody recognizes the mammalian target of rapamycin (mTOR), a member of the phosphatidylinositol 3-kinase-related kinase family of protein kinases. The mTOR protein consists of 2549 amino acids, and has a predicted molecular weight of 289 kDa. mTOR is the core protein of two mTOR complexes (mTORC1 and mTORC2) that drive pathways controlling various fundamental cellular functions, including cell metabolism, growth, migration, and cell death.
产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
实验应用 |
稀释倍数 |
1:500-1:3000 |
1:100-1:1000 |
1:100-1:1000 |
1:100-1:500 |
Assay dependent |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
阳性对照
mouse brain , 293T
预测种属反应
Zebrafish, Sheep(>80% identity)
产品属性
形式
Liquid
存储溶液
PBS, 1% BSA, 20% Glycerol
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
0.38 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant protein encompassing a sequence within the C-terminus region of human mTOR. The exact sequence is proprietary.
纯化方式
Purified by antigen-affinity chromatography.
偶联
Unconjugated
RRID
AB_1241080
注意事项
仅供实验室使用。不适用于人类或动物的任何临床,治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
mechanistic target of rapamycin kinase , FRAP , FRAP1 , FRAP2 , RAFT1 , RAPT1 , SKS
细胞定位
Endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side , Golgi apparatus membrane; Peripheral membrane protein; Cytoplasmic side , Mitochondrion outer membrane; Peripheral membrane protein; Cytoplasmic side
功能与背景
The protein encoded by this gene belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. The ANGPTL7 gene is located in an intron of this gene. [provided by RefSeq]
数据库
研究领域
產品图片
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GTX101557 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with mTOR antibody [C3], C-term (GTX101557) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX101557 IHC-P Image
mTOR antibody [C3], C-term detects mTOR protein at mitochondria on mouse stomach by immunohistochemical analysis. Sample: Paraffin-embedded mouse stomach. mTOR antibody [C3], C-term (GTX101557) diluted at 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX101557 IP Image
mTOR antibody immunoprecipitates mTOR protein in IP experiments. IP Sample: 293T whole cell lysate/extract A : Control with 3 μg of pre-immune rabbit IgG B : Immunoprecipitation of mTOR by 3 μg of mTOR antibody (GTX101557) 5% SDS-PAGE The immunoprecipitated mTOR protein was detected by mTOR antibody (GTX101557) diluted at 1:1000. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.
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GTX101557 WB Image
mTOR antibody detects mTOR protein by western blot analysis. Mouse tissue extracts (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with mTOR antibody (GTX101557) diluted by 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX101557 WB Image
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with mTOR antibody (GTX101557) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
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GTX101557 IHC-P Image
mTOR antibody [C3], C-term detects mTOR protein at cytoplasm in mouse testis by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. mTOR antibody [C3], C-term (GTX101557) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX101557 IHC-P Image
mTOR antibody detects FRAP1 protein at cytosol on human hepatoma by immunohistochemical analysis. Sample: Paraffin-embedded hepatoma. mTOR antibody (GTX101557) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX101557 ICC/IF Image
mTOR antibody [C3], C-term detects mTOR protein at cytoplasm by immunofluorescent analysis.Sample: MCF-7 cells were fixed in ice-cold MeOH for 5 min.Green: mTOR stained by mTOR antibody [C3], C-term (GTX101557) diluted at 1:2000.Blue: Hoechst 33342 staining.Scale bar= 10 μm.
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GTX101557 WB Image
mTOR antibody detects mTOR protein by Western blot analysis. Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with mTOR antibody (GTX101557) diluted at a dilution of 1:1000.
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GTX101557 IP Image
Immunoprecipitation of mTOR protein from 293T whole cell extracts using 5 μg of mTOR antibody [C3], C-term (GTX101557). Western blot analysis was performed using mTOR antibody [C3], C-term (GTX101557). EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
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GTX101557 ChIP assay Image
Cross-linked ChIP was performed with 293T chromatin extract and 5 μg of either control rabbit IgG or anti-mTOR antibody. The precipitated DNA was detected by PCR with primer set targeting to 45S rDNA promoter .
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GTX101557 IHC-P Image
mTOR antibody [C3], C-term detects mTOR protein at cytoplasm by immunohistochemical analysis (Autostainer Formulated).Sample: Paraffin-embedded human colon cancer.mTOR stained by mTOR antibody [C3], C-term (GTX101557) diluted at 1:500.Antigen Retrieval: Citrate buffer, 20 min
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GTX101557 WB Image
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with mTOR antibody [C3], C-term (GTX101557) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX101557 WB Image
Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with mTOR antibody [C3], C-term (GTX101557) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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文献引用
产品评论
mTOR antibody [C3], C-term
目录号 GTX101557
Rating
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( Average 4.7 based on 11 users reviews)
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实验应用
Western Blot(WB)
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( Average 4.6 based on 7 users reviews)
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IHC (Paraffin sections)(IHC-P)
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( Average 5 based on 3 users reviews)
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Immunoprecipitation(IP)
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( Average 5 based on 1 users reviews)
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Date :
Anonymous submitted on 18-Mar-2021
Application Tested :
WB
Sample Species :
Hu
Figure Note :
The data was peer reviewed and published in the journal Biol Open in 2015.
PMID: 25617421
Date :
Anonymous submitted on 18-Mar-2021
Application Tested :
WB
Sample Species :
Ms
Figure Note :
The data was peer reviewed and published in the journal Aging (Albany NY) in 2019.
PMID: 31831718
Date :
Anonymous submitted on 18-Mar-2021
Application Tested :
WB
Sample Species :
Ms
Figure Note :
The data was peer reviewed and published in the journal Aging (Albany NY) in 2019.
PMID: 31831718
Date :
Anonymous submitted on 18-Mar-2021
Application Tested :
IHC-P
Sample Species :
Ms
Figure Note :
The data was peer reviewed and published in the journal Aging (Albany NY) in 2019.
PMID: 31831718
Date :
Anonymous submitted on 18-Mar-2021
Application Tested :
IHC-P
Sample Species :
Ms
Figure Note :
The data was peer reviewed and published in the journal Aging (Albany NY) in 2019.
PMID: 31831718
Date :
Anonymous submitted on 18-Mar-2021
Application Tested :
WB
Sample Species :
Ms
Figure Note :
The data was peer reviewed and published in the journal Aging (Albany NY) in 2019.
PMID: 31831718
Date :
Anonymous submitted on 21-Oct-2020
Application Tested :
IHC-P
Sample Species :
Hu
Sample :
colon cancer tissue
Fixation :
Citrate buffer, 0.3°C
Primary Antibody :
1:500, 4°C, 16Hr
Date :
Anonymous submitted on 30-Jul-2019
Application Tested :
IP
Sample Species :
Hu
Sample :
OVSAYO cells
Amount used :
350000 cells
Blocking :
3% skim milk, 25°C, 0.5Hr
Primary Antibody :
1:500 (based on the volume of cell lysate), 4°C, 8Hr
Figure Note :
Western blot was performed with other mTOR Antibody.
Date :
Anonymous submitted on 08-Sep-2015
Application Tested :
WB
Sample Species :
Ms
Sample :
skin fibroblast tissue
Amount used :
5μg
Blocking :
1% skim milk in TBST, 25°C, 1Hr
Primary Antibody :
1:1000, 4°C, 16Hr
Date :
Anonymous submitted on 03-Jul-2015
Application Tested :
WB
Sample Species :
Hu
Sample :
Flag-human mTOR transfected 293T cells
Amount used :
30μg
Blocking :
3% skim milk in PBS, 25°C, 0.5Hr
Primary Antibody :
1:1000, 4°C, 16Hr
条带说明 :
Lane 1: 293T cells transfected with Flag-human mTOR. Lane 2: 293T cells.
Date :
Anonymous submitted on 19-Oct-2011
Application Tested :
WB
Sample Species :
Rat
Sample :
Neuron primary culture cells
Amount used :
100μg
Blocking :
3% non-fat milk in PBST, 25°C, 2Hr
Primary Antibody :
1:1000, 4°C, 16Hr