mTOR抗体 [C3], C-term

Name: mTOR抗体 [C3], C-term
Brand: GeneTex
SKU: GTX101557
Price: 4000.00 RMB
Rating: 4.4 (5 reviews)

Anti-mTOR antibody [C3], C-term used in Western Blot (WB). GTX101557

Anti-mTOR antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX101557

Anti-mTOR antibody [C3], C-term used in Immunoprecipitation (IP). GTX101557

Anti-mTOR antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101557

Anti-mTOR antibody [C3], C-term used in ChIP assay (ChIP assay). GTX101557

目录号 GTX101557

文献引用 27

宿主	Rabbit
克隆	Polyclonal
同种型	IgG
实验应用	WB, ICC/IF, IHC-P, IP, ChIP assay
种属反应	Human, Mouse, Rat
产品包装	100 μl (¥4000), 25 μl (¥1700)

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View product citations for antibody GTX101557 on CiteAb

产品说明

摘要

mTOR antibody recognizes the mammalian target of rapamycin (mTOR), a member of the phosphatidylinositol 3-kinase-related kinase family of protein kinases. The mTOR protein consists of 2549 amino acids, and has a predicted molecular weight of 289 kDa. mTOR is the core protein of two mTOR complexes (mTORC1 and mTORC2) that drive pathways controlling various fundamental cellular functions, including cell metabolism, growth, migration, and cell death.

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认

实验应用	稀释倍数
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000
IP	1:100-1:500
ChIP assay	Assay dependent

WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

289 kDa. ( Note )

阳性对照

mouse brain , 293T

预测种属反应

Zebrafish, Sheep(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS, 1% BSA, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

0.38 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the C-terminus region of human mTOR. The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_1241080

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

别名

mechanistic target of rapamycin kinase , FRAP , FRAP1 , FRAP2 , RAFT1 , RAPT1 , SKS

细胞定位

Endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side , Golgi apparatus membrane; Peripheral membrane protein; Cytoplasmic side , Mitochondrion outer membrane; Peripheral membrane protein; Cytoplasmic side

功能与背景

The protein encoded by this gene belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. The ANGPTL7 gene is located in an intron of this gene. [provided by RefSeq]

数据库

Gene ID: 2475 MTOR
UniProt: P42345 MTOR

研究领域

產品图片

GTX101557 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with mTOR antibody [C3], C-term (GTX101557) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101557 IHC-P Image

mTOR antibody [C3], C-term detects mTOR protein at mitochondria on mouse stomach by immunohistochemical analysis.
Sample: Paraffin-embedded mouse stomach.
mTOR antibody [C3], C-term (GTX101557) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101557 IP Image

mTOR antibody immunoprecipitates mTOR protein in IP experiments. IP Sample: 293T whole cell lysate/extract A : Control with 3 μg of pre-immune rabbit IgG B : Immunoprecipitation of mTOR by 3 μg of mTOR antibody (GTX101557) 5% SDS-PAGE The immunoprecipitated mTOR protein was detected by mTOR antibody (GTX101557) diluted at 1:1000. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.

GTX101557 WB Image

mTOR antibody detects mTOR protein by western blot analysis. Mouse tissue extracts (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with mTOR antibody (GTX101557) diluted by 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101557 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with mTOR antibody (GTX101557) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

GTX101557 IHC-P Image

mTOR antibody [C3], C-term detects mTOR protein at cytoplasm in mouse testis by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
mTOR antibody [C3], C-term (GTX101557) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX101557 IHC-P Image

mTOR antibody detects FRAP1 protein at cytosol on human hepatoma by immunohistochemical analysis.
Sample: Paraffin-embedded hepatoma.
mTOR antibody (GTX101557) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101557 ICC/IF Image

mTOR antibody [C3], C-term detects mTOR protein at cytoplasm by immunofluorescent analysis.
Sample: MCF-7 cells were fixed in ice-cold MeOH for 5 min.
Green: mTOR stained by mTOR antibody [C3], C-term (GTX101557) diluted at 1:2000.
Blue: Hoechst 33342 staining.
Scale bar= 10 μm.

GTX101557 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with mTOR antibody [C3], C-term (GTX101557) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101557 WB Image

Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with mTOR antibody [C3], C-term (GTX101557) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101557 WB Image

mTOR antibody detects mTOR protein by Western blot analysis. Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with mTOR antibody (GTX101557) diluted at a dilution of 1:1000.

GTX101557 IP Image

Immunoprecipitation of mTOR protein from 293T whole cell extracts using 5 μg of mTOR antibody [C3], C-term (GTX101557).
Western blot analysis was performed using mTOR antibody [C3], C-term (GTX101557).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX101557 ChIP assay Image

Cross-linked ChIP was performed with 293T chromatin extract and 5 μg of either control rabbit IgG or anti-mTOR antibody. The precipitated DNA was detected by PCR with primer set targeting to 45S rDNA promoter .

GTX101557 IHC-P Image

mTOR antibody [C3], C-term detects mTOR protein at cytoplasm by immunohistochemical analysis (Autostainer Formulated).
Sample: Paraffin-embedded human colon cancer.
mTOR stained by mTOR antibody [C3], C-term (GTX101557) diluted at 1:500.
Antigen Retrieval: Citrate buffer, 20 min