产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:500-1:3000 |
| Assay dependent |
| Assay dependent |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
产品属性
形式
Liquid
存储溶液
PBS
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
2 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant fragment of human mTOR.
纯化方式
Affinity purified by Protein A.
偶联
Unconjugated
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
mechanistic target of rapamycin kinase , FRAP , FRAP1 , FRAP2 , RAFT1 , RAPT1 , SKS
细胞定位
Endoplasmic reticulum membrane , Golgi apparatus membrane , Mitochondrion outer membrane , Lysosome , Cytoplasm
功能与背景
The protein encoded by this gene belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. The ANGPTL7 gene is located in an intron of this gene. [provided by RefSeq, Sep 2008]
数据库
研究领域
產品图片
![Anti-mTOR antibody [HL2216] used in Western Blot (WB). GTX638220](/templates/default/js/lightbox/images/loading.gif "Anti-mTOR antibody [HL2216] used in Western Blot (WB). GTX638220")
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GTX638220 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with mTOR antibody [HL2216] (GTX638220) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX638220 ICC/IF Image
mTOR antibody [HL2216] detects mTOR protein at Golgi apparatus and nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: mTOR stained by mTOR antibody [HL2216] (GTX638220) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.Blue: Fluoroshield with DAPI (GTX30920).
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GTX638220 WB Image
Various tissue extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with mTOR antibody [HL2216] (GTX638220) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.
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GTX638220 IHC-P Image
mTOR antibody [HL2216] detects mTOR protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse duodenum.mTOR stained by mTOR antibody [HL2216] (GTX638220) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX638220 WB Image
Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with mTOR antibody [HL2216] (GTX638220) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
|
|
|
GTX638220 WB Image
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with mTOR antibody [HL2216] (GTX638220) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
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GTX638220 WB Image
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with mTOR antibody [HL2216] (GTX638220) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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文献引用
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产品评论
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![Anti-mTOR antibody [HL2216] used in Western Blot (WB). GTX638220](/upload/website/prouct_img/small/GTX638220/GTX638220_45026_20230428_WB_shRNA_watermark_23050223_501.webp "Anti-mTOR antibody [HL2216] used in Western Blot (WB). GTX638220")
![Anti-mTOR antibody [HL2216] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638220](/upload/website/prouct_img/small/GTX638220/GTX638220_T-44942_20230331_ICC_IF_23041023_397.webp "Anti-mTOR antibody [HL2216] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638220")
![Anti-mTOR antibody [HL2216] used in Western Blot (WB). GTX638220](/upload/website/prouct_img/small/GTX638220/GTX638220_T-44942_20230224_WB_M_R_23030219_565.webp "Anti-mTOR antibody [HL2216] used in Western Blot (WB). GTX638220")
![Anti-mTOR antibody [HL2216] used in IHC (Paraffin sections) (IHC-P). GTX638220](/upload/website/prouct_img/small/GTX638220/GTX638220_T-44942_20230303_IHC-P_M_23031402_537.webp "Anti-mTOR antibody [HL2216] used in IHC (Paraffin sections) (IHC-P). GTX638220")
![Anti-mTOR antibody [HL2216] used in Western Blot (WB). GTX638220](/upload/website/prouct_img/small/GTX638220/GTX638220_45026_20230428_WB_R_23050223_914.webp "Anti-mTOR antibody [HL2216] used in Western Blot (WB). GTX638220")
![Anti-mTOR antibody [HL2216] used in Western Blot (WB). GTX638220](/upload/website/prouct_img/small/GTX638220/GTX638220_45026_20230428_WB_TPM_watermark_23050223_189.webp "Anti-mTOR antibody [HL2216] used in Western Blot (WB). GTX638220")
![Anti-mTOR antibody [HL2216] used in Western Blot (WB). GTX638220](/upload/website/prouct_img/small/GTX638220/GTX638220_45026_20230428_WB_M_23050223_860.webp "Anti-mTOR antibody [HL2216] used in Western Blot (WB). GTX638220")
![Anti-mTOR antibody [HL2216] used in Western Blot (WB). GTX638220](/upload/website/prouct_img/normal/GTX638220/GTX638220_45026_20230428_WB_shRNA_watermark_23050223_501.webp "Anti-mTOR antibody [HL2216] used in Western Blot (WB). GTX638220")
![Anti-mTOR antibody [HL2216] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638220](/upload/website/prouct_img/normal/GTX638220/GTX638220_T-44942_20230331_ICC_IF_23041023_397.webp "Anti-mTOR antibody [HL2216] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638220")
![Anti-mTOR antibody [HL2216] used in Western Blot (WB). GTX638220](/upload/website/prouct_img/normal/GTX638220/GTX638220_T-44942_20230224_WB_M_R_23030219_565.webp "Anti-mTOR antibody [HL2216] used in Western Blot (WB). GTX638220")
![Anti-mTOR antibody [HL2216] used in IHC (Paraffin sections) (IHC-P). GTX638220](/upload/website/prouct_img/normal/GTX638220/GTX638220_T-44942_20230303_IHC-P_M_23031402_537.webp "Anti-mTOR antibody [HL2216] used in IHC (Paraffin sections) (IHC-P). GTX638220")
![Anti-mTOR antibody [HL2216] used in Western Blot (WB). GTX638220](/upload/website/prouct_img/normal/GTX638220/GTX638220_45026_20230428_WB_R_23050223_914.webp "Anti-mTOR antibody [HL2216] used in Western Blot (WB). GTX638220")
![Anti-mTOR antibody [HL2216] used in Western Blot (WB). GTX638220](/upload/website/prouct_img/normal/GTX638220/GTX638220_45026_20230428_WB_TPM_watermark_23050223_189.webp "Anti-mTOR antibody [HL2216] used in Western Blot (WB). GTX638220")
![Anti-mTOR antibody [HL2216] used in Western Blot (WB). GTX638220](/upload/website/prouct_img/normal/GTX638220/GTX638220_45026_20230428_WB_M_23050223_860.webp "Anti-mTOR antibody [HL2216] used in Western Blot (WB). GTX638220")
