Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with p16 ARC antibody (GTX115342) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using ARPC5(GTX115342) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min