产品说明
摘要
p21 (Cip1) antibody recognizes p21Cip1 protein, a cyclin-dependent kinase inhibitor with a predicted molecular weight of 18 kDa. p21 is a major downstream regulator of p53 activity in response to DNA damage to control cell cycle arrest. p21 also interacts with proliferating cell nuclear antigen (PCNA) to inhibit PCNA-dependent S-phase DNA synthesis.
产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
实验应用 |
稀释倍数 |
1:500-1:3000 |
1:100-1:1000 |
Assay dependent |
Assay dependent |
1:100-1:500 |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
阳性对照
HCT116 (30 μM Cisplatin treatment for 24 hr) , HCT116 (30 μM Cisplatin treatment for 48 hr)
预测种属反应
Sheep, Bovine, Cat, Rhesus Monkey(>80% identity)
产品属性
形式
Liquid
存储溶液
PBS
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1.45 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant protein encompassing a sequence within the center region of human p21 Cip1. The exact sequence is proprietary.
纯化方式
Affinity purified by Protein A.
偶联
Unconjugated
RRID
AB_2888121
注意事项
仅供实验室使用。不适用于人类或动物的任何临床,治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
cyclin dependent kinase inhibitor 1A , CAP20 , CDKN1 , CIP1 , MDA-6 , P21 , SDI1 , WAF1 , p21CIP1
细胞定位
Cytoplasm , Nucleus
功能与背景
This gene encodes a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes, and thus functions as a regulator of cell cycle progression at G1. The expression of this gene is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen (PCNA), a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation. Two alternatively spliced variants, which encode an identical protein, have been reported. [provided by RefSeq]
数据库
研究领域
產品图片
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GTX629543 WB Image
Wild-type (WT) and p21 Cip1 knockout (KO) HeLa cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with p21 Cip1 antibody [GT1032] (GTX629543) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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GTX629543 WB Image
p21 Cip1 antibody [GT1032] detects p21 Cip1 protein by western blot analysis. A. 30 μg HCT116 whole cell lysate/extract (untreated) B. 30 μg HCT116 whole cell lysate/extract (30 μM Cisplatin treatment for 24 hr) C. 30 μg HCT116 whole cell lysate/extract (30 μM Cisplatin treatment for 48 hr) 15% SDS-PAGE p21 Cip1 antibody [GT1032] (GTX629543) dilution: 1:1000 The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX629543 IP Image
p21 Cip1 antibody [GT1032] immunoprecipitates CDKN1A protein in IP experiments. IP samples: HCT-116 whole cell extract treat with 30uM cisplatin for 48 hr A. 30 μg HCT-116 whole cell extract treat with 30uM cisplatin for 48 hr B. Control with 4 μg of preimmune Mouse IgG C. Immunoprecipitation of CDKN1A protein by 4 μg p21 Cip1 antibody [GT1032] (GTX629543) 15 % SDS-PAGE The immunoprecipitated CDKN1A protein was detected by p21 Cip1 antibody [GT1032] (GTX629543) diluted at 1:1000. [EasyBlot anti-mouse IgG (GTX221667-01) was used as a secondary reagent]
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GTX629543 ICC/IF Image
p21 Cip1 antibody [GT1032] detects p21 Cip1 protein at nucleus by immunofluorescent analysis.Sample: Mock and treated HCT116 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: p21 Cip1 stained by p21 Cip1 antibody [GT1032] (GTX629543) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar= 10 μm.
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GTX629543 ICC/IF Image
p21 Cip1 antibody [GT1032] detects p21 Cip1 protein at nucleus by immunofluorescent analysis. Sample: MCF7 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CDK4 protein stained by CDK4 antibody (GTX102993) diluted at 1:1000. Red: p21 Cip1 protein stained by p21 Cip1 antibody [GT1032] (GTX629543) diluted at 1:500. Blue: Hoechst 33342 staining.
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GTX629543 FACS Image
p21 Cip1 antibody [GT1032] (GTX629543) detects p21 Cip1 by flow cytometry analysis. Sample: HepG2 cell. Black: Unlabelled sample was used as a control. Red: p21 Cip1 antibody [GT1032] (GTX629543) dilution: 1:50. Acquisition of 20,000 events were collected for FACS analysis.
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GTX629543 IHC-P Image
p21 Cip1 antibody [GT1032] detects p21 Cip1 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded human lung cancer.p21 Cip1 stained by p21 Cip1 antibody [GT1032] (GTX629543) diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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文献引用
产品评论
p21 Cip1 antibody [GT1032]
目录号 GTX629543
Rating
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( Average 4.7 based on 7 users reviews)
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实验应用
Western Blot(WB)
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( Average 4.7 based on 6 users reviews)
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Immunocytochemistry/ Immunofluorescence(ICC/IF)
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( Average 5 based on 1 users reviews)
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Date :
Anonymous submitted on 18-Mar-2021
Application Tested :
WB
Sample Species :
Hu
Figure Note :
The data was peer reviewed and published in the journal PLoS One in 2015.
PMID: 25961745
Date :
Anonymous submitted on 18-Mar-2021
Application Tested :
WB
Sample Species :
Hu
Figure Note :
The data was peer reviewed and published in the journal PLoS One in 2015.
PMID: 25961745
Date :
Anonymous submitted on 18-Mar-2021
Application Tested :
WB
Sample Species :
Hu
Figure Note :
The data was peer reviewed and published in the journal PLoS One in 2015.
PMID: 25961745
Date :
Anonymous submitted on 14-Oct-2020
Application Tested :
WB
Sample Species :
Hu
Sample :
HepG2 cells
Amount used :
20 μg
Blocking :
5% non-fat milk in TBST, 22°C, 0.5Hr
Primary Antibody :
1:2000, 22°C, 18Hr
Date :
Anonymous submitted on 13-Mar-2019
Application Tested :
WB
Sample Species :
Hu
Sample :
KOPN79 cells
Amount used :
20 μg
Blocking :
5% skim milk in TBST, 25°C, 1Hr
Primary Antibody :
1:1000, 4°C, 16Hr
Date :
Anonymous submitted on 03-Jan-2019
Application Tested :
ICC/IF
Sample Species :
Hu
Sample :
MIA PaCa-2 cells
Blocking :
3% BSA, 25°C, 0.3Hr
Fixation :
4% paraformaldehyde
Permeabilization /Antigen retrieval :
0.8% Triton X-100 in 3% BSA
Primary Antibody :
1:100, 4°C, 16Hr
Date :
Anonymous submitted on 27-Aug-2013
Application Tested :
WB
Sample Species :
Hu
Sample :
HCT116 cells
Amount used :
30μg
Blocking :
1%BSA in PBST, 4°C, 1Hr
Primary Antibody :
1:3000, 4°C, 16Hr