产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 0.1-0.3μg/ml |
| 5-10μg/ml |
|
Note :
IHC-P
Human Prostate shows nuclear staining of secretory cells.
|
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
阳性对照
The peptide used to generate this antibody is available for purchase (GTX89392-PEP).
预测种属反应
Mouse, Rat, Bovine, Cat, Dog, Pig(>80% identity)
产品属性
形式
Liquid
存储溶液
TBS, 0.5% BSA
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Peptide with sequence C-KRPATDDSSTQNK, from the internal region of the protein sequence according to NP_004055.1.
纯化方式
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
偶联
Unconjugated
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
cyclin dependent kinase inhibitor 1B , CDKN4 , KIP1 , MEN1B , MEN4 , P27KIP1
细胞定位
Nucleus , Cytoplasm , Endosome
功能与背景
This gene encodes a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. The encoded protein binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state. Mutations in this gene are associated with multiple endocrine neoplasia type IV (MEN4). [provided by RefSeq, Apr 2014]
数据库
研究领域
產品图片
|
GTX89392 WB Image
WB analysis of human prostate lysate using GTX89392 p27 Kip1 antibody, Internal.
Dilution : 0.1μg/ml
Loading : 35μg protein in RIPA buffer
|
|
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GTX89392 IHC-P Image
IHC-P analysis of human prostate using GTX89392 p27 Kip1 antibody, Internal.
Antigen retrieval : citrate buffer pH 6
Dilution : 5μg/ml
|
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