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p38 MAPK抗体 [N1C3-2]

KO/KD Orthogonal
Anti-p38 MAPK antibody [N1C3-2] used in Western Blot (WB). GTX110720
Anti-p38 MAPK antibody [N1C3-2] used in Western Blot (WB). GTX110720
Anti-p38 MAPK antibody [N1C3-2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110720
Anti-p38 MAPK antibody [N1C3-2] used in IHC (Paraffin sections) (IHC-P). GTX110720
Anti-p38 MAPK antibody [N1C3-2] used in IHC (Paraffin sections) (IHC-P). GTX110720
Anti-p38 MAPK antibody [N1C3-2] used in Western Blot (WB). GTX110720
Anti-p38 MAPK antibody [N1C3-2] used in Western Blot (WB). GTX110720
Anti-p38 MAPK antibody [N1C3-2] used in Western Blot (WB). GTX110720

目录号 GTX110720

文献引用 34
2

宿主

 Rabbit

克隆

 Polyclonal

同种型

 IgG

实验应用

 WB, ICC/IF, IHC-P

种属反应

 Human, Mouse, Rat, Dog
产品包装
100 μl (¥4000),
25 μl (¥1700)
查看所有 p38 MAPK 的产品

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

41 kDa. ( Note )

阳性对照

293T

预测种属反应

Sheep, Bovine, Xenopus tropicalis, Chimpanzee(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS, 1% BSA, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

0.44 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Full length human p38 MAPK Recombinant protein.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_10617720

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

mitogen-activated protein kinase 14 , CSBP , CSBP1 , CSBP2 , CSPB1 , EXIP , Mxi2 , PRKM14 , PRKM15 , RK , SAPK2A , p38 , p38ALPHA

细胞定位

Cytoplasm , Nucleus

功能与背景

The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various environmental stresses and proinflammatory cytokines. The activation requires its phosphorylation by MAP kinase kinases (MKKs), or its autophosphorylation triggered by the interaction of MAP3K7IP1/TAB1 protein with this kinase. The substrates of this kinase include transcription regulator ATF2, MEF2C, and MAX, cell cycle regulator CDC25B, and tumor suppressor p53, which suggest the roles of this kinase in stress related transcription and cell cycle regulation, as well as in genotoxic stress response. Four alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq]

数据库

研究领域

產品图片

Anti-p38 MAPK antibody [N1C3-2] used in Western Blot (WB). GTX110720

GTX110720 WB Image

KO/KD

Wild-type (WT) and p38 MAPK knockout (KO) 293T cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with p38 MAPK antibody [N1C3-2] (GTX110720) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-p38 MAPK antibody [N1C3-2] used in Western Blot (WB). GTX110720

GTX110720 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with p38 MAPK antibody [N1C3-2] (GTX110720) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-p38 MAPK antibody [N1C3-2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110720

GTX110720 ICC/IF Image

p38 MAPK antibody [N1C3-2] detects p38 MAPK protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde for 10 min.
Green: p38 MAPK protein stained by p38 MAPK antibody [N1C3-2] (GTX110720) diluted at 1:100.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-p38 MAPK antibody [N1C3-2] used in IHC (Paraffin sections) (IHC-P). GTX110720

GTX110720 IHC-P Image

p38 MAPK antibody [N1C3-2] detects p38 MAPK protein at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
p38 MAPK antibody [N1C3-2] (GTX110720) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-p38 MAPK antibody [N1C3-2] used in IHC (Paraffin sections) (IHC-P). GTX110720

GTX110720 IHC-P Image

p38 antibody [N1C3-2] detects p38 protein at nucleus on human lung adenocarcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human lung adenocarcinoma.
p38 antibody [N1C3-2] (GTX110720) diluted at 1:250.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-p38 MAPK antibody [N1C3-2] used in Western Blot (WB). GTX110720

GTX110720 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with p38 MAPK antibody (GTX110720) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-p38 MAPK antibody [N1C3-2] used in Western Blot (WB). GTX110720

GTX110720 WB Image

The data was published in the journal Mar Drugs in 2019. PMID: 31213027

Anti-p38 MAPK antibody [N1C3-2] used in Western Blot (WB). GTX110720

GTX110720 WB Image

The data was published in the journal Int J Mol Sci in 2018. PMID: 29518977

文献引用

Application Reference

Lee SC et al. Pharmaceuticals (Basel) 2023; 16 (9) Synergistic Effect of Ginsenoside Rh2 Combines with Ionizing Radiation on CT26/luc Colon Carcinoma Cells and Tumor-Bearing Animal Model.
Zhang X et al. Chin Herb Med 2022; 14 (4) : 554-562 Taxifolin attenuates inflammation via suppressing MAPK signal pathway in vitro and in silico analysis.
实验应用 : WB
种属反应 : Mouse
Fu L et al. Bioengineered 2022; 13 (5) : 12847-12862 Deleted in lymphocytic leukemia 2 induces retinoic acid receptor beta promoter methylation and mitogen activated kinase-like protein activation to enhance viability and mobility of colorectal cancer cells.
实验应用 : WB
种属反应 : Human
Qian X et al. Bioengineered 2022; 13 (5) : 12583-12597 EZH2 enhances proliferation and migration of trophoblast cell lines by blocking GADD45A-mediated p38/MAPK signaling pathway.
Hsu YC et al. Oxid Med Cell Longev 2022; 2022 : 1380353 Administration of N-Acetylcysteine to Regress the Fibrogenic and Proinflammatory Effects of Oxidative Stress in Hypertrophic Ligamentum Flavum Cells.
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产品评论

p38 MAPK antibody [N1C3-2] 目录号 GTX110720

Rating
( Average 5 based on 2 users reviews)
实验应用
Western Blot(WB)
( Average 5 based on 2 users reviews)
Anti-p38 MAPK antibody [N1C3-2] used in Western Blot (WB). GTX110720
Date : Anonymous submitted on 05-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Sample : SCC4 cells
Amount used : 30 μg
Blocking : 5% non-fat dry milk in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 12Hr
Figure Note : 5 μM ADC treatment for 0-120 min.
Anti-p38 MAPK antibody [N1C3-2] used in Western Blot (WB). GTX110720
Date : Anonymous submitted on 08-Jan-2018
Score :
Application Tested : WB
Sample Species : Hu
Amount used : 25 μg
Blocking : 5% milk in TBST, 25°C, 1Hr
Primary Antibody : 1:500, 4°C, 16Hr
条带说明 : Lane 1: PANC1, Lane 2: AsPC1, Lane 3: BxPC3
我要评论产品
Application Reference
Lee SC et al. Pharmaceuticals (Basel) 2023; 16 (9) Synergistic Effect of Ginsenoside Rh2 Combines with Ionizing Radiation on CT26/luc Colon Carcinoma Cells and Tumor-Bearing Animal Model.
Zhang X et al. Chin Herb Med 2022; 14 (4):554-562 Taxifolin attenuates inflammation via suppressing MAPK signal pathway in vitro and in silico analysis.
实验应用 : WB
种属反应 : Mouse
Fu L et al. Bioengineered 2022; 13 (5):12847-12862 Deleted in lymphocytic leukemia 2 induces retinoic acid receptor beta promoter methylation and mitogen activated kinase-like protein activation to enhance viability and mobility of colorectal cancer cells.
实验应用 : WB
种属反应 : Human
Qian X et al. Bioengineered 2022; 13 (5):12583-12597 EZH2 enhances proliferation and migration of trophoblast cell lines by blocking GADD45A-mediated p38/MAPK signaling pathway.
Hsu YC et al. Oxid Med Cell Longev 2022; 2022:1380353 Administration of N-Acetylcysteine to Regress the Fibrogenic and Proinflammatory Effects of Oxidative Stress in Hypertrophic Ligamentum Flavum Cells.
Yan Sun et al. Immun Inflamm Dis 2022; 10 (7):e641 TRPM7 promotes lipopolysaccharide-induced inflammatory dysfunction in renal tubular epithelial cells.
实验应用 : WB
种属反应 : Human
Jihee Hong et al. J Ginseng Res 2022; 46 (3):481-488 Ginsenoside Rg1 suppresses cancer cell proliferation through perturbing mitotic progression.
实验应用 : WB
Cui X et al. Dis Model Mech 2022; (Epub) Exercise suppresses mouse systemic AApoAII amyloidosis through enhancement of the p38 MAPK signaling pathway.
实验应用 : WB
种属反应 : Mouse
Kuo-Hao Ho et al. Neurotherapeutics 2021; 18 (2):1371-1392 Guanabenz Sensitizes Glioblastoma Cells to Sunitinib by Inhibiting GADD34-Mediated Autophagic Signaling.
实验应用 : WB
种属反应 : Human
Yu-Jen Chiu et al. Oncol Rep 2021; 46 (1) Next?generation sequencing analysis reveals that MTH?3, a novel curcuminoid derivative, suppresses the invasion of MDA?MB?231 triple?negative breast adenocarcinoma cells.
实验应用 : WB
种属反应 : Human
Chi-Cheng Lu et al. Exp Ther Med 2021; 22 (2):822 Dracorhodin perchlorate enhances wound healing via β-catenin, ERK/p38, and AKT signaling in human HaCaT keratinocytes.
实验应用 : WB
种属反应 : Human
Li Y et al. Transl Lung Cancer Res 2021; 10 (4):1841-1856 Let-7b-3p inhibits tumor growth and metastasis by targeting the BRF2-mediated MAPK/ERK pathway in human lung adenocarcinoma.
实验应用 : WB
种属反应 : Human
Liu JF et al. J Cell Physiol 2020; (Epub) PM2.5 facilitates IL-6 production in human osteoarthritis synovial fibroblasts via ASK1 activation.
实验应用 : WB
种属反应 : Human
Lu CC et al. Int J Oncol 2019; 54 (4):1271-1281
Gwon D et al. Mol Cells 2019; 42 (12):840-849 c-Cbl Acts as an E3 Ligase Against DDA3 for Spindle Dynamics and Centriole Duplication during Mitosis.
实验应用 : WB
种属反应 : Human
Yi-Feng Yang et al. Chinese Journal of Physiology 2019; 62 (4):139-147 Adenine decreases hypertrophic effects through interleukin-18 receptor.
实验应用 : WB
种属反应 : Rat
Jan JS et al. Mar Drugs 2019; 17 (6) Hirsutanol A Attenuates Lipopolysaccharide-Mediated Matrix Metalloproteinase 9 Expression and Cytokines Production and Improves Endotoxemia-Induced Acute Sickness Behavior and Acute Lung Injury.
实验应用 : WB
种属反应 : Human
Jau?Tien Lin et al. Journal of Food Biochemistry 2019; (Epub) Litchi (Litchi chinensis Sonn.) flower proanthocyanidin fraction exhibited protective efficacy to suppress nickel?induced expression for vascular endothelial growth factor in HepG2 cells.
实验应用 : WB
种属反应 : Human
Li HY et al. J Agric Food Chem 2019; 67 (7):1889-1901 Melatonin Inhibits in Vitro Smooth Muscle Cell Inflammation and Proliferation and Atherosclerosis in Apolipoprotein E-Deficient Mice.
实验应用 : WB
种属反应 : Mouse
Qi J et al. Front Mol Neurosci 2018; 11:281 Sulbactam Protects Hippocampal Neurons Against Oxygen-Glucose Deprivation by Up-Regulating Astrocytic GLT-1 via p38 MAPK Signal Pathway.
Chen CF et al. Oncol Lett 2018; 16 (5):6323-6330
Liu YH et al. Phytomedicine 2018; 46:184-192 Aqueous extracts of Paeonia suffruticosa modulates mitochondrial proteostasis by reactive oxygen species-induced endoplasmic reticulum stress in pancreatic cancer cells.
实验应用 : WB
种属反应 : Human
Farhat F et al. Open Access Maced J Med Sci 2018; 6 (11):1982-1985 Correlation of P38 Mitogen-Activated Protein Kinase Expression to Clinical Stage in Nasopharyngeal Carcinoma.
实验应用 : IHC-P
种属反应 : Human
Chen CF et al. Oncology Letters 2018;:6323-6330 synergistic inhibitory effects of * and curcumin on human *-resistant oral cancer car cells through intrinsic apoptotic process.
实验应用 : WB
种属反应 : Human
Bonfante R et al. Neuroscience 2018; 391:120-130 The P2X7 Receptor, Cathepsin S and Fractalkine in the Trigeminal Subnucleus Caudalis Signal Persistent Hypernociception in Temporomandibular Rat Joints.
实验应用 : WB
种属反应 : Rat
Lin JT et al. Journal of Functional Foods 2018; 47:343-349 Protective effect and mechanism of Muntingia calabura Linn. fruit ethanolic extract against vascular endothelial growth factor production in nickel-stimulated hepatocellular carcinoma cells.
实验应用 : WB
种属反应 : Human
Sarin N et al. Int J Mol Sci 2018; 19 (3) key players of * resistance: towards a systems pharmacology approach.
实验应用 : WB
种属反应 : Human
Lin JT et al. Food Funct. 2017; 8 (3):1245-1253 Molecular mechanisms of the effects of the ethanolic extract of Muntingia calabura Linn. fruit on lipopolysaccharide-induced pro-inflammatory mediators in macrophages.
实验应用 : WB
Yeh CJ et al. Sci Rep 2017; 7 (1):15599 The effects of artocarpin on wound healing: in vitro and in vivo studies.
实验应用 : WB
种属反应 : Human
Chen CC et al. PLoS One 2016; 11 (7):e0158662 The Anti-Inflammatory Effects and Mechanisms of Eupafolin in Lipopolysaccharide-Induced Inflammatory Responses in RAW264.7 Macrophages.
实验应用 : WB
种属反应 : Mouse
Chen PH et al. Neuropharmacology 2016; 110 (Pt A):362-75 The miR-204-3p-targeted IGFBP2 pathway is involved in xanthohumol-induced glioma cell apoptotic death.
实验应用 : WB
种属反应 : Human
Chuang YF et al. PLoS One 2015; 10 (8):e0137177 Protein Phosphatase 2A in Lipopolysaccharide-Induced Cyclooxygenase-2 Expression in Murine Lymphatic Endothelial Cells.
实验应用 : WB
种属反应 : Mouse
Vordenbaumen S et al. BMC Immunol 2013; 14:46 Human casein alpha s1 (CSN1S1) skews in vitro differentiation of monocytes towards macrophages.
实验应用 : WB
种属反应 : Human
Kang KH et al. Eur J Cancer 2013; 49 (12):2747-59 Enhancement role of host 12/15-lipoxygenase in melanoma progression.
实验应用 : WB
种属反应 : Mouse
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
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