应用说明
*最佳稀释倍数与浓度应由研究人员确认
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
阳性对照
UV treated HeLa extracts
预测种属反应
Sheep, Bovine, Dog, Xenopus tropicalis, Chimpanzee(>80% identity)
形式
Liquid
存储溶液
PBS, 1% BSA, 20% Glycerol
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
0.3 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Carrier-protein conjugated synthetic peptide surrounding phospho Thr180/Tyr182 of human p38 MAPK. The exact sequence is proprietary.
纯化方式
Purified by antigen-affinity chromatography.
偶联
Unconjugated
RRID
AB_2886996
注意事项
仅供实验室使用。不适用于人类或动物的任何临床,治疗或诊断用途。不适合动物或人类食用。
别名
mitogen-activated protein kinase 14 , CSBP , CSBP1 , CSBP2 , CSPB1 , EXIP , Mxi2 , PRKM14 , PRKM15 , RK , SAPK2A , p38 , p38ALPHA
细胞定位
Cytoplasm , Nucleus
功能与背景
The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various environmental stresses and proinflammatory cytokines. The activation requires its phosphorylation by MAP kinase kinases (MKKs), or its autophosphorylation triggered by the interaction of MAP3K7IP1/TAB1 protein with this kinase. The substrates of this kinase include transcription regulator ATF2, MEF2C, and MAX, cell cycle regulator CDC25B, and tumor suppressor p53, which suggest the roles of this kinase in stress related transcription and cell cycle regulation, as well as in genotoxic stress response. Four alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq]
数据库
研究领域