产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
实验应用 |
稀释倍数 |
Assay dependent |
1:100-1:1900 |
Assay dependent |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
预测种属反应
Rat, Rabbit, Sheep, Bovine, Cat, Dog, Guinea pig, Pig, Rhesus Monkey(>80% identity)
产品属性
形式
Liquid
存储溶液
PBS
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Carrier-protein conjugated synthetic peptide surrounding mutant R175H of human p53. The exact sequence is proprietary.
纯化方式
Affinity purified by Protein A.
偶联
Unconjugated
RRID
AB_2909974
注意事项
仅供实验室使用。不适用于人类或动物的任何临床,治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
tumor protein p53 , BCC7 , BMFS5 , LFS1 , P53 , TRP53
细胞定位
Cytoplasm,Nucleus,Endoplasmic reticulum,Mitochondrion matrix
功能与背景
This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons from identical transcript variants (PMIDs: 12032546, 20937277). [provided by RefSeq, Dec 2016]
数据库
研究领域
產品图片
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GTX636395 WB Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with p53 (R175H Mutant) antibody [HL1129] (GTX636395) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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GTX636395 IHC-P Image
p53 (R175H Mutant) antibody [HL1129] detects p53 (R175H Mutant) protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded AU565 Xenograft.p53 (R175H Mutant) stained by p53 (R175H Mutant) antibody [HL1129] (GTX636395) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX636395 ICC/IF Image
p53 (R175H Mutant) antibody [HL1129] detects p53 (R175H Mutant) protein at nucleus by immunofluorescent analysis.Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: p53 (R175H Mutant) stained by p53 (R175H Mutant) antibody [HL1129] (GTX636395) diluted at 1:1900.Blue: Fluoroshield with DAPI (GTX30920).
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GTX636395 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with p53 (R175H Mutant) antibody [HL1129] (GTX636395) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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文献引用
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