产品说明
摘要
p53 antibody recognizes p53 protein, which has a predicted molecular weight of 44 kDa but is typically detected by western blot at 53 kDa. p53, known widely as a tumor suppressor, plays critical roles in various cellular processes including cell cycle regulation, DNA repair, and apoptosis. p53 is mutated in many human cancers.
产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
实验应用 |
稀释倍数 |
1:500-1:10000 |
Assay dependent |
1:1000 |
Assay dependent |
Assay dependent |
1:100-1:500 |
Assay dependent |
Note :
IHC-P
Antigen retrieval can be done with EDTA at pH8.0 - Some customers have stated that the product works well without antigen retrieval.
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WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
阳性对照
293T , A431 , HCT116 , 293T (0.5 μM doxorubicin treatment for 24 hr) , HCT116(30 μM cisplatin treatment for 24 hr)
产品属性
形式
Liquid
存储溶液
1XPBS (pH7), 20% Glycerol
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
0.25 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant human p53 expressed in E. coli.
纯化方式
Protein A purified
From tissue culture supernatant
偶联
Unconjugated
RRID
AB_370118
注意事项
仅供实验室使用。不适用于人类或动物的任何临床,治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
tumor protein p53 , BCC7 , BMFS5 , LFS1 , P53 , TRP53
细胞定位
Cytoplasm , Nucleus , Endoplasmic reticulum , Mitochondrion matrix
功能与背景
This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons from identical transcript variants (PMIDs: 12032546, 20937277). [provided by RefSeq, Dec 2016]
数据库
研究领域
產品图片
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GTX70214 WB Image
Non-transfected (–) and transfected (+) HCT116 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with p53 antibody [DO1] (GTX70214) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX70214 IHC-P Image
p53 antibody [DO1] detects p53 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded human pancreatic cancer.p53 stained by p53 antibody [DO1] (GTX70214) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX70214 ICC/IF Image
p53 antibody [DO1] detects p53 protein at nucleus by immunofluorescent analysis.Sample: Mock and treated HCT116 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: p53 stained by p53 antibody [DO1] (GTX70214) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
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GTX70214 IHC-P Image
p53 antibody [DO1] detects p53 protein at cytoplasm and nucleus by immunohistochemical analysis.Sample: Paraffin-embedded human breast carcinoma.p53 stained by p53 antibody [DO1] (GTX70214) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX70214 WB Image
293T whole cell extracts (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with p53 antibody [DO1] (GTX70214) diluted at 1:40000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX70214 IP Image
Immunoprecipitation of p53 protein from 293T whole cell extracts using 5 μg of p53 antibody [DO1] (GTX70214). Western blot analysis was performed using p53 antibody [D01] (GTX70214). EasyBlot anti-Mouse IgG (GTX221667-01) was used as a secondary reagent.
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GTX70214 WB Image
Untreated (–) and treated (+) HCT116 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with p53 antibody [DO1] (GTX70214) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX70214 IHC-P Image
p53 antibody [DO1] detects p53 protein at cytoplasm and nucleus by immunohistochemical analysis.Sample: Paraffin-embedded human colon cancer.p53 stained by p53 antibody [DO1] (GTX70214) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX70214 IHC-P Image
p53 antibody [DO1] detects p53 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human SW480 xenograft. p53 stained by p53 antibody [DO1] (GTX70214) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX70214 IHC-P Image
p53 antibody [DO1] detects p53 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human NCI-H1299 xenograft. p53 stained by p53 antibody [DO1] (GTX70214) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX70214 WB Image
Untreated (–) and treated (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with p53 antibody [DO1] (GTX70214) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX70214 IHC-P Image
p53 antibody [DO1] detects p53 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human oral carcinoma. p53 stained by p53 antibody [DO1] (GTX70214) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX70214 WB Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with p53 antibody [DO1] (GTX70214) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX70214 WB Image
The data was published in the journal PLoS One in 2014. PMID: 24933654
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文献引用
产品评论
p53 antibody [DO1]
目录号 GTX70214
Rating
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( Average 5 based on 2 users reviews)
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实验应用
Western Blot(WB)
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( Average 5 based on 2 users reviews)
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Date :
Anonymous submitted on 10-Aug-2021
Application Tested :
WB
Sample Species :
Hu
Sample :
U2OS cells
Amount used :
30 μg
Blocking :
5% non-fat dry milk in TBST, 25°C, 1Hr
Primary Antibody :
1:1000, 4°C, 12Hr
条带说明 :
untreated-5 μM cisplatin treatment for 24hr.
Date :
Anonymous submitted on 06-May-2016
Application Tested :
WB
Sample Species :
Hu
Sample :
OS-RC-2 cells
Amount used :
30μg
Blocking :
25°C, 1Hr
Primary Antibody :
1:5000, 4°C, 16Hr