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p53抗体 [HL2242]

Recombinant antibody KO/KD Orthogonal
Anti-p53 antibody [HL2242] used in Western Blot (WB). GTX638291
Anti-p53 antibody [HL2242] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638291
Anti-p53 antibody [HL2242] used in Western Blot (WB). GTX638291
Anti-p53 antibody [HL2242] used in Western Blot (WB). GTX638291
Anti-p53 antibody [HL2242] used in Western Blot (WB). GTX638291
Anti-p53 antibody [HL2242] used in IHC (Paraffin sections) (IHC-P). GTX638291
Anti-p53 antibody [HL2242] used in IHC (Paraffin sections) (IHC-P). GTX638291
Anti-p53 antibody [HL2242] used in Western Blot (WB). GTX638291

目录号 GTX638291

1

宿主

 Rabbit

克隆

 Monoclonal

克隆编号

 HL2242

同种型

 IgG

实验应用

 WB, ICC/IF, IHC-P

种属反应

 Human, Mouse, Cat, Dog
产品包装
100 μl (¥4000),
25 μl (¥1700)
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产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

44 kDa. ( Note )

产品属性

形式

Liquid

存储溶液

PBS

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant fragmemt of human p53.

纯化方式

Affinity purified by Protein A.

偶联

Unconjugated

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

tumor protein p53 , BCC7 , BMFS5 , LFS1 , P53 , TRP53

细胞定位

Cytoplasm,Nucleus,Endoplasmic reticulum,Mitochondrion matrix

功能与背景

This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons from identical transcript variants (PMIDs: 12032546, 20937277). [provided by RefSeq, Dec 2016]

数据库

研究领域

產品图片

Anti-p53 antibody [HL2242] used in Western Blot (WB). GTX638291

GTX638291 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with p53 antibody [HL2242] (GTX638291) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-p53 antibody [HL2242] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638291

GTX638291 ICC/IF Image

p53 antibody [HL2242] detects p53 protein at nucleus by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: p53 stained by p53 antibody [HL2242] (GTX638291) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-p53 antibody [HL2242] used in Western Blot (WB). GTX638291

GTX638291 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with p53 antibody [HL2242] (GTX638291) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-p53 antibody [HL2242] used in Western Blot (WB). GTX638291

GTX638291 WB Image

Orthogonal

Untreated (–) and treated (+) HCT-116 whole cell extract (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with p53 antibody [HL2242] (GTX638291) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-p53 antibody [HL2242] used in Western Blot (WB). GTX638291

GTX638291 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with p53 antibody [HL2242] (GTX638291) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-p53 antibody [HL2242] used in IHC (Paraffin sections) (IHC-P). GTX638291

GTX638291 IHC-P Image

p53 antibody [HL2242] detects p53 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
p53 stained by p53 antibody [HL2242] (GTX638291) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-p53 antibody [HL2242] used in IHC (Paraffin sections) (IHC-P). GTX638291

GTX638291 IHC-P Image

p53 antibody [HL2242] detects p53 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human oral carcinoma.
p53 stained by p53 antibody [HL2242] (GTX638291) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-p53 antibody [HL2242] used in Western Blot (WB). GTX638291

GTX638291 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with p53 antibody [HL2242] (GTX638291) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

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产品评论

p53 antibody [HL2242] 目录号 GTX638291

Rating
( Average 5 based on 1 users reviews)
实验应用
Western Blot(WB)
( Average 5 based on 1 users reviews)
Anti-p53 antibody [HL2242] used in Western Blot (WB). GTX638291
Date : Anonymous submitted on 29-Oct-2023
Score :
Application Tested : WB
Sample Species : Ms
Sample : Neuro2A, ID8-6, ID8-1, ID8-4 cells
Amount used : 30μg
Blocking : 5% non-fat milk in TBST, 25°C, 0.5Hr
Primary Antibody : 1:1000, 4°C, > 16Hr
Figure Note : The signal was developed with Trident femto ECL, GTX14698.
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产品包装 报价 (¥)
¥ 4000
¥ 1700
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