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p53抗体

Orthogonal
Anti-p53 antibody used in Western Blot (WB). GTX128135
Anti-p53 antibody used in Western Blot (WB). GTX128135

目录号 GTX128135

文献引用 26
2

宿主

 Rabbit

克隆

 Polyclonal

同种型

 IgG

实验应用

 WB

种属反应

 Zebrafish
产品包装
100 μl (¥4000),
25 μl (¥1700)
查看所有 zebrafish p53 的产品

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

42 kDa. ( Note )

阳性对照

3dpf zebrafish embryos treated with UVC

产品属性

形式

Liquid

存储溶液

PBS, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1.47 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Zebrafish

免疫原

Recombinant protein encompassing a sequence within the N-terminus region of zebrafish p53. The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_2864277

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

tumor protein p53 , brp53 , drp53 , etID22686.5 , fb40d06 , p53 , wu:fb40d06 , zgc:111919

细胞定位

Cytoplasm , Nucleus

功能与背景

Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of bax and fas antigen expression, or by repression of Bcl-2 expression.

数据库

研究领域

產品图片

Anti-p53 antibody used in Western Blot (WB). GTX128135

GTX128135 WB Image

Orthogonal

Untreated (–) and treated (+) Zebrafish embryos extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with p53 antibody (GTX128135) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-p53 antibody used in Western Blot (WB). GTX128135

GTX128135 WB Image

Orthogonal

The data was published in the journal Sci Rep in 2015.PMID: 26507500

文献引用

Application Reference

Y Han et al. EMBO J 2024; A Mettl16/m6A/mybl2b/Igf2bp1 axis ensures cell cycle progression of embryonic hematopoietic stem and progenitor cells
实验应用 : WB
种属反应 : Zebrafish
Varineau JE et al. Dis Model Mech 2024; 17 (2) A common cellular response to broad splicing perturbations is characterized by metabolic transcript downregulation driven by the Mdm2-p53 axis.
CW Cheng et al. Cancers (Basel) 2024; 16 (3) : 657 miR-622 Increases miR-30a Expression through Inhibition of Hypoxia-Inducible Factor 1α to Improve Metastasis and Chemoresistance in Human Invasive Breast Cancer Cells
实验应用 : WB
Sun K et al. iScience 2023; 26 (11) : 108103 The splicing factor DHX38 enables retinal development through safeguarding genome integrity.
Chen J et al. MedComm (2020) 2023; 4 (5) : e352 Identification of the natural chalcone glycoside hydroxysafflor yellow A as a suppressor of P53 overactivation-associated hematopoietic defects.
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产品评论

p53 antibody 目录号 GTX128135

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( Average 5 based on 2 users reviews)
实验应用
Anti-p53 antibody. GTX128135
Date : Anonymous submitted on 25-Dec-2017
Score :
Sample Species : Zfsh
Sample : Eye tumor tissue
Blocking : Normal goat serum, 0.5Hr
Permeabilization /Antigen retrieval : EDTA pH 9.0
Primary Antibody : 1:700, 4°C, 16Hr
Figure Note : Zebrafish p53 mutant eye tumor, p53 nuclear staining, magnification 200x (A) and 400x (B).
Anti-p53 antibody. GTX128135
Date : Anonymous submitted on 25-Dec-2017
Score :
Sample Species : Zfsh
Sample : Kidney tissue
Blocking : Normal goat serum, 0.5Hr
Permeabilization /Antigen retrieval : EDTA pH 9.0
Primary Antibody : 1:700, 4°C, 16Hr
Figure Note : Zebrafish p53 mutant kidney, positive for nuclear staining, magnification 40x (A) and 400x (B).
我要评论产品
Application Reference
Y Han et al. EMBO J 2024; A Mettl16/m6A/mybl2b/Igf2bp1 axis ensures cell cycle progression of embryonic hematopoietic stem and progenitor cells
实验应用 : WB
种属反应 : Zebrafish
Varineau JE et al. Dis Model Mech 2024; 17 (2) A common cellular response to broad splicing perturbations is characterized by metabolic transcript downregulation driven by the Mdm2-p53 axis.
CW Cheng et al. Cancers (Basel) 2024; 16 (3):657 miR-622 Increases miR-30a Expression through Inhibition of Hypoxia-Inducible Factor 1α to Improve Metastasis and Chemoresistance in Human Invasive Breast Cancer Cells
实验应用 : WB
Sun K et al. iScience 2023; 26 (11):108103 The splicing factor DHX38 enables retinal development through safeguarding genome integrity.
Chen J et al. MedComm (2020) 2023; 4 (5):e352 Identification of the natural chalcone glycoside hydroxysafflor yellow A as a suppressor of P53 overactivation-associated hematopoietic defects.
Parija M et al. Oncotarget 2023; 14:466-480 G-protein-coupled receptor 141 mediates breast cancer proliferation and metastasis by regulating oncogenic mediators and the p-mTOR/p53 axis.
Babu S et al. Elife 2022; 11 Banp regulates DNA damage response and chromosome segregation during the cell cycle in zebrafish retina.
实验应用 : WB
种属反应 : Zebrafish
Jingzhen Li et al. Nucleic Acids Res 2021; 49 (4):2027-2043 Prpf31 is essential for the survival and differentiation of retinal progenitor cells by modulating alternative splicing
实验应用 : WB
种属反应 : Zebrafish
Shoffner A et al. Cell Rep 2020; 32 (9):108089 Tp53 Suppression Promotes Cardiomyocyte Proliferation during Zebrafish Heart Regeneration.
Espanola SG et al. Nat Cell Biol 2020; 22 (12):1411-1422 Haematopoietic stem cell-dependent Notch transcription is mediated by p53 through the Histone chaperone Supt16h.
实验应用 : WB
种属反应 : Zebrafish
Mohapatra S et al. Cell Death Differ 2020; (Epub) Estrogen and estrogen receptors chauffeur the sex-biased autophagic action in liver.
实验应用 : WB
Lu LF et al. Fish Shellfish Immunol 2020; 102:449-459 Ca2+ plays an antiviral role by increasing p53 expression to achieve protection against spring viraemia of carp virus infection.
实验应用 : WB
种属反应 : Zebrafish
Mawed SA et al. Int J Mol Sci 2020; 21 (4) Strategy of Hepatic Metabolic Defects Induced by beclin1 Heterozygosity in Adult Zebrafish.
实验应用 : WB
种属反应 : Zebrafish
Adriana Petrovici et al. J. Mar. Sci. Eng. 2020; 8 (2):73 Toxicity of Deltamethrin to Zebrafish Gonads Revealed by Cellular Biomarkers.
种属反应 : Zebrafish
Caihong Wang et al. Chemosphere 2020; 241 Triclosan-induced liver and brain injury in zebrafish (Danio rerio) via abnormal expression of miR-125 regulated by PKCα/Nrf2/p53 signaling pathways.
种属反应 : Zebrafish
D'Agati G et al. Dis Model Mech 2019; 12 (7) Active receptor tyrosine kinases
Li H et al. J Cell Physiol 2019; (Epub) Mitochondrial proteomics reveals the mechanism of spermatogenic cells apoptosis induced by carbon ion radiation in zebrafish.
实验应用 : WB
种属反应 : Zebrafish
Liu PH et al. Nat Cell Biol 2019; 21 (2):203-213 An IRAK1-PIN1 signalling axis drives intrinsic tumour resistance to radiation therapy.
实验应用 : WB
种属反应 : Zebrafish
Si J et al. Toxicology 2018; (Epub) Effects of ionizing radiation and HLY78 on the zebrafish embryonic developmental toxicity.
实验应用 : WB
种属反应 : Zebrafish
Shtraizent N et al. Elife 2017; 6 MPI depletion enhances O-GlcNAcylation of p53 and suppresses the Warburg effect.
实验应用 : WB
种属反应 : Zebrafish
Zhou R et al. Mutat Res 2016; 793-794:41-50 Effects of Ru(CO)3Cl-glycinate on the developmental toxicities induced by X-ray and carbon-ion irradiation in zebrafish embryos.
实验应用 : WB
种属反应 : Zebrafish
Wei Y et al. Development 2016; (Epub) RNA polymerase III component Rpc9 regulates hematopoietic stem and progenitor cell maintenance in zebrafish.
实验应用 : WB
种属反应 : Zebrafish
Zhang S et al. Invest Ophthalmol Vis Sci 2016; 57 (4):1991-2000 Antiviral Drug * Is a Potent Inhibitor of the Proliferation of Mller Glia-Derived Progenitors During Zebrafish Retinal Regeneration.
实验应用 : WB
种属反应 : Zebrafish
Jing J et al. Sci Rep 2015; 5:15906 A feedback regulatory loop involving p53/miR-200 and * endocrine axis controls embryo size of zebrafish.
实验应用 : WB
种属反应 : Zebrafish
Percival SM et al. Dis Model Mech 2015; 8 (8):941-55 Variations in dysfunction of sister chromatid cohesion in esco2 mutant zebrafish reflect the phenotypic diversity of Roberts syndrome.
实验应用 : WB
种属反应 : Zebrafish
Tsai M et al. Dev Neurobiol 2014; 75 (9):908-26 Modulation of p53 and met expression by kr-ppel-like factor 8 regulates zebrafish cerebellar development.
实验应用 : WB
种属反应 : Zebrafish
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
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