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p54nrb抗体

Anti-p54nrb antibody used in IHC (Paraffin sections) (IHC-P). GTX101419
Anti-p54nrb antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101419
Anti-p54nrb antibody used in IHC (Paraffin sections) (IHC-P). GTX101419
Anti-p54nrb antibody used in Western Blot (WB). GTX101419

目录号 GTX101419

宿主

Rabbit

克隆

Polyclonal

同种型

IgG

实验应用

WB, ICC/IF, IHC-P

种属反应

Human, Mouse
产品包装
100 μl (¥4000),
25 μl (¥1700)

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

54 kDa. ( Note )

阳性对照

HepG2

预测种属反应

Rat, Bovine, Chicken, Xenopus laevis, Chimpanzee(>80% identity)

产品属性

形式

Liquid

存储溶液

0.1M Tris, 0.1M Glycine, 10% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

0.72 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the N-terminus region of human p54nrb. The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_2037534

注意事项

仅供实验室使用。不适用于人类或动物的任何临床,治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

non-POU domain containing octamer binding , MRXS34 , NMT55 , NRB54 , P54 , P54NRB , PPP1R114

细胞定位

Nucleus

功能与背景

This gene encodes an RNA-binding protein which plays various roles in the nucleus, including transcriptional regulation and RNA splicing. A rearrangement between this gene and the transcription factor E3 gene has been observed in papillary renal cell carcinoma. Alternatively spliced transcript variants have been described. Pseudogenes exist on Chromosomes 2 and 16. [provided by RefSeq]

数据库

研究领域

產品图片

Anti-p54nrb antibody used in IHC (Paraffin sections) (IHC-P). GTX101419

GTX101419 IHC-P Image

p54/nrb antibody detects p54/nrb protein at nucleus in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
p54/nrb antibody (GTX101419) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-p54nrb antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101419

GTX101419 ICC/IF Image

p54/nrb antibody detects p54/nrb protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: p54/nrb protein stained by p54/nrb antibody (GTX101419) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Hoechst 33342 staining.

Anti-p54nrb antibody used in IHC (Paraffin sections) (IHC-P). GTX101419

GTX101419 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using p54/nrb (GTX101419) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-p54nrb antibody used in Western Blot (WB). GTX101419

GTX101419 WB Image

Sample (30 ug of whole cell lysate)
A: Hep G2 (GTX27900)
10% SDS PAGE
GTX101419 diluted at 1:1000

文献引用

产品评论

p54nrb antibody 目录号 GTX101419

Rating ( Average 5 based on 1 users reviews)
实验应用
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 21-Mar-2018
Score :
Application Tested : WB
Sample Species : Hu
Sample : T-Rex of HEK293T cells (clone G11)
Amount used : 18 μg
Blocking : 5% skim milk in TBT, 21°C, 1.5Hr
Primary Antibody : 1:1000, 21°C, 1.5Hr
条带说明 : Western blot analysis of the soluble (sup) and insoluble (ppt) fractions of Non-transfected (–) and transfected (+) T-Rex of HEK293T.
Figure Note : Image courtesy of Shintaro Iwashita from Tokushima Bunri University at Kagawa.
SDS
Glycerol.pdf
Thimerosal.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
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