产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:500 - 1:2000 |
| 1:50 - 1:200 |
| 1:50 - 1:200 |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
产品属性
形式
Liquid
存储溶液
PBS, 50% Glycerol
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
Batch dependent (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human NONO (NP_001138881.1).
纯化方式
Purified by affinity chromatography
偶联
Unconjugated
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
non-POU domain containing octamer binding , MRXS34 , NMT55 , NRB54 , P54 , P54NRB , PPP1R114
细胞定位
Nucleus,Nucleus speckle,Nucleolus
功能与背景
This gene encodes an RNA-binding protein which plays various roles in the nucleus, including transcriptional regulation and RNA splicing. A rearrangement between this gene and the transcription factor E3 gene has been observed in papillary renal cell carcinoma. Alternatively spliced transcript variants have been described. Pseudogenes exist on Chromosomes 2 and 16. [provided by RefSeq, Feb 2009]
数据库
研究领域
產品图片
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GTX54596 ICC/IF Image
ICC/IF analysis of C6 cells using GTX54596 p54nrb antibody.
Blue : DAPI
Dilution : 1:100
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GTX54596 IHC-P Image
IHC-P analysis of rat lung tissue using GTX54596 p54nrb antibody.
Dilution : 1:100
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GTX54596 IHC-P Image
IHC-P analysis of human lung cancer tissue using GTX54596 p54nrb antibody.
Dilution : 1:100
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GTX54596 IHC-P Image
IHC-P analysis of rat liver tissue using GTX54596 p54nrb antibody.
Dilution : 1:100
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GTX54596 IHC-P Image
IHC-P analysis of human thyroid cancer tissue using GTX54596 p54nrb antibody.
Dilution : 1:100
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GTX54596 WB Image
WB analysis of various sample lysates using GTX54596 p54nrb antibody.
Dilution : 1:1000
Loading : 25μg per lane
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GTX54596 ICC/IF Image
ICC/IF analysis of U2OS cells using GTX54596 p54nrb antibody.
Blue : DAPI
Dilution : 1:100
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GTX54596 ICC/IF Image
ICC/IF analysis of NIH/3T3 cells using GTX54596 p54nrb antibody.
Blue : DAPI
Dilution : 1:100
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GTX54596 IHC-P Image
IHC-P analysis of mouse brain tissue using GTX54596 p54nrb antibody.
Dilution : 1:100
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GTX54596 IHC-P Image
IHC-P analysis of mouse kidney tissue using GTX54596 p54nrb antibody.
Dilution : 1:100
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