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p63抗体 [HL1027]

Recombinant antibody KO/KD
Anti-p63 antibody [HL1027] used in Western Blot (WB). GTX635841
Anti-p63 antibody [HL1027] used in Western Blot (WB). GTX635841
Anti-p63 antibody [HL1027] used in IHC (Paraffin sections) (IHC-P). GTX635841
Anti-p63 antibody [HL1027] used in IHC (Whole mount) (IHC-Wm). GTX635841
Anti-p63 antibody [HL1027] used in Western Blot (WB). GTX635841
Anti-p63 antibody [HL1027] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635841
Anti-p63 antibody [HL1027] used in IHC (Paraffin sections) (IHC-P). GTX635841

目录号 GTX635841

宿主

 Rabbit

克隆

 Monoclonal

克隆编号

 HL1027

同种型

 IgG

实验应用

 WB, ICC/IF, IHC-P, IHC-Wm

种属反应

 Human, Mouse, Zebrafish
产品包装
100 μl (¥4000),
25 μl (¥1700)
查看所有 p63 的产品

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P Assay dependent
IHC-Wm Assay dependent
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

77 kDa. ( Note )

预测种属反应

Rat, Bovine, Chicken(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the center region of human p63. The exact sequence is proprietary.

纯化方式

Affinity purified by Protein A.

偶联

Unconjugated

RRID

AB_2909908

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

tumor protein p63 , AIS , B(p51A) , B(p51B) , EEC3 , KET , LMS , NBP , OFC8 , RHS , SHFM4 , TP53CP , TP53L , TP73L , p40 , p51 , p53CP , p63 , p73H , p73L

细胞定位

Nucleus

功能与背景

This gene encodes a member of the p53 family of transcription factors. The functional domains of p53 family proteins include an N-terminal transactivation domain, a central DNA-binding domain and an oligomerization domain. Alternative splicing of this gene and the use of alternative promoters results in multiple transcript variants encoding different isoforms that vary in their functional properties. These isoforms function during skin development and maintenance, adult stem/progenitor cell regulation, heart development and premature aging. Some isoforms have been found to protect the germline by eliminating oocytes or testicular germ cells that have suffered DNA damage. Mutations in this gene are associated with ectodermal dysplasia, and cleft lip/palate syndrome 3 (EEC3); split-hand/foot malformation 4 (SHFM4); ankyloblepharon-ectodermal defects-cleft lip/palate; ADULT syndrome (acro-dermato-ungual-lacrimal-tooth); limb-mammary syndrome; Rap-Hodgkin syndrome (RHS); and orofacial cleft 8. [provided by RefSeq, Aug 2016]

数据库

研究领域

產品图片

Anti-p63 antibody [HL1027] used in Western Blot (WB). GTX635841

GTX635841 WB Image

KO/KD

Non-transfected (–) and transfected (+) A431 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with p63 antibody [HL1027] (GTX635841) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-p63 antibody [HL1027] used in Western Blot (WB). GTX635841

GTX635841 WB Image

Whole zebrafish extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with p63 antibody [HL1027] (GTX635841) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-p63 antibody [HL1027] used in IHC (Paraffin sections) (IHC-P). GTX635841

GTX635841 IHC-P Image

p63 antibody [HL1027] detects p63 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human placenta.
p63 stained by p63 antibody [HL1027] (GTX635841) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-p63 antibody [HL1027] used in IHC (Whole mount) (IHC-Wm). GTX635841

GTX635841 IHC-Wm Image

p63 antibody [HL1027] detects p63 protein on whole mount zebrafish by immunohistochemical analysis.
Sample: Paraformaldehyde-fixed 2 days-post-fertilization zebrafish embryo.
Green: p63 stained by p63 antibody [HL1027] (GTX635841) diluted at 1:100.
Antigen Retrieval: Tris-HCl buffer, pH 9.0, 20 min at 70ºC

Anti-p63 antibody [HL1027] used in Western Blot (WB). GTX635841

GTX635841 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with p63 antibody [HL1027] (GTX635841) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-p63 antibody [HL1027] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635841

GTX635841 ICC/IF Image

p63 antibody [HL1027] detects p63 protein at nucleus by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: p63 stained by p63 antibody [HL1027] (GTX635841) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.

Anti-p63 antibody [HL1027] used in IHC (Paraffin sections) (IHC-P). GTX635841

GTX635841 IHC-P Image

p63 antibody [HL1027] detects p63 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
p63 stained by p63 antibody [HL1027] (GTX635841) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

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产品包装 报价 (¥)
¥ 4000
¥ 1700
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