产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:500-1:3000 |
| Assay dependent |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
预测种属反应
Rabbit, Bovine, Chicken, Xenopus tropicalis(>80% identity)
产品属性
形式
Liquid
存储溶液
PBS
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Carrier-protein conjugated synthetic peptide surrounding phospho Thr421/Ser424 of human p70 S6K. The exact sequence is proprietary.
纯化方式
Affinity purified by Protein A.
偶联
Unconjugated
RRID
AB_2888544
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
ribosomal protein S6 kinase B1 , PS6K , S6K , S6K-beta-1 , S6K1 , STK14A , p70 S6KA , p70(S6K)-alpha , p70-S6K , p70-alpha
细胞定位
Mitochondrion outer membrane,Mitochondrion,Cytoplasm
功能与背景
This gene encodes a member of the ribosomal S6 kinase family of serine/threonine kinases. The encoded protein responds to mTOR (mammalian target of rapamycin) signaling to promote protein synthesis, cell growth, and cell proliferation. Activity of this gene has been associated with human cancer. Alternatively spliced transcript variants have been observed. The use of alternative translation start sites results in isoforms with longer or shorter N-termini which may differ in their subcellular localizations. There are two pseudogenes for this gene on chromosome 17. [provided by RefSeq, Jan 2013]
数据库
研究领域
產品图片
![Anti-p70 S6K (phospho Thr421/Ser424) antibody [HL129] used in Western Blot (WB). GTX635621](/templates/default/js/lightbox/images/loading.gif "Anti-p70 S6K (phospho Thr421/Ser424) antibody [HL129] used in Western Blot (WB). GTX635621")
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GTX635621 WB Image
Wild-type (WT) and p70 S6K (phospho Thr421/Ser424) knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with p70 S6K (phospho Thr421/Ser424) antibody [HL129] (GTX635621) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX635621 IHC-P Image
p70 S6K (phospho Thr421/Ser424) antibody [HL5] detects p70 S6K (phospho Thr421/Ser424) protein at cytoplasm by immunohistochemical analysis in the presence of control peptide (left) or antigen-specific peptide (right).Sample: Paraffin-embedded mouse intestine.p70 S6K (phospho Thr421/Ser424) stained by p70 S6K (phospho Thr421/Ser424) antibody [HL5] (GTX635621) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX635621 WB Image
Untreated (–) and treated (+) Rat2 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with p70 S6K (phospho Thr421/Ser424) antibody [HL129] (GTX635621) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX635621 WB Image
Untreated (–) and treated (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with p70 S6K (phospho Thr421/Ser424) antibody [HL129] (GTX635621) diluted at 1:500 and competitor's antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.
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GTX635621 WB Image
Untreated (–) and treated (+) Rat2 whole cell extracts (50 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with p70 S6K (phospho Thr421/Ser424) antibody [HL129] (GTX635621) diluted at 1:500 and competitor's antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.
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GTX635621 WB Image
Untreated (–) and treated (+) Neuro2A whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with p70 S6K (phospho Thr421/Ser424) antibody [HL5] (GTX635621) diluted at 1:500 and competitor's antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
*The competitor is not affiliated with GeneTex and does not endorse this product.
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GTX635621 IHC-P Image
p70 S6K (phospho Thr421/Ser424) antibody [HL5] detects p70 S6K (phospho Thr421/Ser424) protein by immunohistochemical analysis.Sample: Paraffin-embedded rat pancreas.p70 S6K (phospho Thr421/Ser424) stained by p70 S6K (phospho Thr421/Ser424) antibody [HL5] (GTX635621) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX635621 IHC-P Image
p70 S6K (phospho Thr421/Ser424) antibody [HL5] detects p70 S6K (phospho Thr421/Ser424) protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse tissue.p70 S6K (phospho Thr421/Ser424) stained by p70 S6K (phospho Thr421/Ser424) antibody [HL5] (GTX635621) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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文献引用
目前没有使用 p70 S6K (phospho Thr421/Ser424) antibody [HL129] (GTX635621) 的文献被收录。快来回报我们第一篇参考文献吧！
产品评论
p70 S6K (phospho Thr421/Ser424) antibody [HL129]
目录号 GTX635621
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Rating
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( Average 3 based on 1 users reviews)
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实验应用
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Western Blot(WB)
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( Average 3 based on 1 users reviews)
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Date :
Anonymous submitted on 19-Oct-2021
Application Tested :
WB
Sample Species :
Hu
Sample :
HepG2 cells
Amount used :
25μg
Blocking :
5% non-fat milk, BSA in TBST, 4°C, 1Hr
Primary Antibody :
1:1000, 4°C, 16Hr
![Anti-p70 S6K (phospho Thr421/Ser424) antibody [HL129] used in Western Blot (WB). GTX635621](/upload/website/prouct_img/small/GTX635621/GTX635621_43978_20220128_WB_KO_watermark_w_23061202_779.webp "Anti-p70 S6K (phospho Thr421/Ser424) antibody [HL129] used in Western Blot (WB). GTX635621")
![Anti-p70 S6K (phospho Thr421/Ser424) antibody [HL129] used in IHC (Paraffin sections) (IHC-P). GTX635621](/upload/website/prouct_img/small/GTX635621/GTX635621_43978_20220429_IHC-P_antigen neutralize_w_23061202_328.webp "Anti-p70 S6K (phospho Thr421/Ser424) antibody [HL129] used in IHC (Paraffin sections) (IHC-P). GTX635621")
![Anti-p70 S6K (phospho Thr421/Ser424) antibody [HL129] used in Western Blot (WB). GTX635621](/upload/website/prouct_img/small/GTX635621/GTX635621_43978_20220304_WB_R_treatment_EGF_peptideblocking_w_23061202_350.webp "Anti-p70 S6K (phospho Thr421/Ser424) antibody [HL129] used in Western Blot (WB). GTX635621")
![Anti-p70 S6K (phospho Thr421/Ser424) antibody [HL129] used in Western Blot (WB). GTX635621](/upload/website/prouct_img/small/GTX635621/GTX635621_43978_20200626_WB_treatment_FBS_competitor_watermark_w_23061202_820.webp "Anti-p70 S6K (phospho Thr421/Ser424) antibody [HL129] used in Western Blot (WB). GTX635621")
![Anti-p70 S6K (phospho Thr421/Ser424) antibody [HL129] used in Western Blot (WB). GTX635621](/upload/website/prouct_img/small/GTX635621/GTX635621_43978_20201008_WB_R_treatment_EGF_competitor_watermark_w_23061202_952.webp "Anti-p70 S6K (phospho Thr421/Ser424) antibody [HL129] used in Western Blot (WB). GTX635621")
![Anti-p70 S6K (phospho Thr421/Ser424) antibody [HL129] used in Western Blot (WB). GTX635621](/upload/website/prouct_img/small/GTX635621/GTX635621_43978_20200807_WB_M_treatment_EGF_competitor_watermark_w_23061202_318.webp "Anti-p70 S6K (phospho Thr421/Ser424) antibody [HL129] used in Western Blot (WB). GTX635621")
![Anti-p70 S6K (phospho Thr421/Ser424) antibody [HL129] used in IHC (Paraffin sections) (IHC-P). GTX635621](/upload/website/prouct_img/small/GTX635621/GTX635621_43978_20221228_IHC-P_R_22122901_791.webp "Anti-p70 S6K (phospho Thr421/Ser424) antibody [HL129] used in IHC (Paraffin sections) (IHC-P). GTX635621")
![Anti-p70 S6K (phospho Thr421/Ser424) antibody [HL129] used in IHC (Paraffin sections) (IHC-P). GTX635621](/upload/website/prouct_img/small/GTX635621/GTX635621_43978_20221228_IHC-P_multiple_M_22122901_513.webp "Anti-p70 S6K (phospho Thr421/Ser424) antibody [HL129] used in IHC (Paraffin sections) (IHC-P). GTX635621")
![Anti-p70 S6K (phospho Thr421/Ser424) antibody [HL129] used in Western Blot (WB). GTX635621](/upload/website/prouct_img/normal/GTX635621/GTX635621_43978_20220128_WB_KO_watermark_w_23061202_779.webp "Anti-p70 S6K (phospho Thr421/Ser424) antibody [HL129] used in Western Blot (WB). GTX635621")
![Anti-p70 S6K (phospho Thr421/Ser424) antibody [HL129] used in IHC (Paraffin sections) (IHC-P). GTX635621](/upload/website/prouct_img/normal/GTX635621/GTX635621_43978_20220429_IHC-P_antigen neutralize_w_23061202_328.webp "Anti-p70 S6K (phospho Thr421/Ser424) antibody [HL129] used in IHC (Paraffin sections) (IHC-P). GTX635621")
![Anti-p70 S6K (phospho Thr421/Ser424) antibody [HL129] used in Western Blot (WB). GTX635621](/upload/website/prouct_img/normal/GTX635621/GTX635621_43978_20220304_WB_R_treatment_EGF_peptideblocking_w_23061202_350.webp "Anti-p70 S6K (phospho Thr421/Ser424) antibody [HL129] used in Western Blot (WB). GTX635621")
![Anti-p70 S6K (phospho Thr421/Ser424) antibody [HL129] used in Western Blot (WB). GTX635621](/upload/website/prouct_img/normal/GTX635621/GTX635621_43978_20200626_WB_treatment_FBS_competitor_watermark_w_23061202_820.webp "Anti-p70 S6K (phospho Thr421/Ser424) antibody [HL129] used in Western Blot (WB). GTX635621")
![Anti-p70 S6K (phospho Thr421/Ser424) antibody [HL129] used in Western Blot (WB). GTX635621](/upload/website/prouct_img/normal/GTX635621/GTX635621_43978_20201008_WB_R_treatment_EGF_competitor_watermark_w_23061202_952.webp "Anti-p70 S6K (phospho Thr421/Ser424) antibody [HL129] used in Western Blot (WB). GTX635621")
![Anti-p70 S6K (phospho Thr421/Ser424) antibody [HL129] used in Western Blot (WB). GTX635621](/upload/website/prouct_img/normal/GTX635621/GTX635621_43978_20200807_WB_M_treatment_EGF_competitor_watermark_w_23061202_318.webp "Anti-p70 S6K (phospho Thr421/Ser424) antibody [HL129] used in Western Blot (WB). GTX635621")
![Anti-p70 S6K (phospho Thr421/Ser424) antibody [HL129] used in IHC (Paraffin sections) (IHC-P). GTX635621](/upload/website/prouct_img/normal/GTX635621/GTX635621_43978_20221228_IHC-P_R_22122901_791.webp "Anti-p70 S6K (phospho Thr421/Ser424) antibody [HL129] used in IHC (Paraffin sections) (IHC-P). GTX635621")
![Anti-p70 S6K (phospho Thr421/Ser424) antibody [HL129] used in IHC (Paraffin sections) (IHC-P). GTX635621](/upload/website/prouct_img/normal/GTX635621/GTX635621_43978_20221228_IHC-P_multiple_M_22122901_513.webp "Anti-p70 S6K (phospho Thr421/Ser424) antibody [HL129] used in IHC (Paraffin sections) (IHC-P). GTX635621")
![Anti-p70 S6K (phospho Thr421/Ser424) antibody [HL129] used in Western Blot (WB). GTX635621](/upload/website/support/rewardsprogram/normal/genetex_GTX635621_WB_1_20211019_23072402_136.webp "Anti-p70 S6K (phospho Thr421/Ser424) antibody [HL129] used in Western Blot (WB). GTX635621")
